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Showing 1 to 20 of 33 for “"single molecule FRET"”.
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Single-molecule FRET analysis of the dynamics of AMPA receptor
Single-molecule fluorescence resonance energy transfer (smFRET) spectroscopy has advanced to become a powerful tool in characterizing biomolecules to the molecular level. In this thesis, the smFRET technique was utilized for the first time to analyze the dynamics of the conformational changes of …
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Single-molecule FRET study on the RecA-mediated DNA repair
… genomic integrity through recombination. Using single-molecule assays and hidden Markov modeling, we show the most direct evidence that a RecA filament grows and shrinks primarily, one monomer at a time only from the extremities. Both ends grow and shrink, contrary to earlier predictions, and a …
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Force manipulation and single molecule FRET of transcriptional regulatory factors
Made available in DSpace on 2013-02-03T19:17:18Z (GMT). No. of bitstreams: 2 Michael_Brenner.pdf: 48140044 bytes, checksum: 737009cfe6f7c863e8be1a53bd84ce23 (MD5) license.txt: 4065 bytes, checksum: 7b3880bb6f2daecb451ca65aadeab0b2 (MD5)
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Single Molecule Fret Studies of DNA Binding and RNA Polymerizing Proteins
Submitted by Megan O'Donnell (mnodonn2@illinois.edu) on 2011-11-07T16:47:35Z No. of bitstreams: 1 2007_roy_biophysics.pdf: 6758862 bytes, checksum: 83953b72a305a817a6a698b6e9d6fb86 (MD5)
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Acquisition and analysis of DNA recombination intermediates using single molecule FRET microscopy
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-23T20:45:46Z No. of bitstreams: 1 McKinney_Sean.pdf: 2035766 bytes, checksum: 414180e8b31f044c02a9ee30c58f9b8f (MD5)
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Single molecule fret study on the mechanism of RecA mediated strand exchange
… reaction, RecA forms a helical filament on single stranded (ss) DNA that searches for homology and exchanges complementary base pairs with a homologous double strand (ds) DNA to form a new heteroduplex. The study of strand exchange in ensemble assays is limited by the diffusion limited …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… and dynamics of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy Transfer (smFRET) techniques that provide information about heterogeneity and dynamic behaviors of molecules have been developed to investigate inter- and intra-molecular interactions. Here we have used …
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Characterization of protein induced fluorescence enhancement (PIFE): A label-free protein assay with short distance sensitivity
Single molecule FRET (Förster Resonance Energy Transfer) has been recently widely used for monitoring the interactions between a protein and a nucleic acid. It typically requires a site-specific labeling of the protein, which can be labor-intensive and inefficient. In addition, for common dye …
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Exploring the Structural Dynamics of Bacterial Chemotaxis
… with signaling in the cytoplasmic domain. Single-molecule Förster Resonance Energy Transfer (FRET), which provides a nanometer scale ruler that enables the observation of protein conformations and dynamics, was applied to intact, functional chemoreceptor homodimers to observe ligand-induced …
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A single molecule study on the structural basis of ion selective permeation in voltage-gated sodium channels
… of the NavAb channel can be examined utilizing single-molecule FRET. It is proposed that the ion selectivity of VGSCs is based on the size and charge, implying very minimized flexibilities in the selectivity filter. However, it was found that selectivity collapse underlies the slow inactivation …
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
… study three members of the replication system: single-stranded DNA, single-stranded DNA binding proteins, and the sliding clamp and clamp loader proteins. Fluorescence studies have been widely used to study the properties of biomolecules and here we will use both Fluorescence Polarization …
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Advances in myotonic dystrophy type 1 drug discovery through design of novel ligands and mechanism establishment
… we describe the development of a bioactive small molecule (Chapter 2) and a small library of dimeric ligands (Chapter 3) leading to an optimized bivalent ligand. These novel ligands target CUGexp RNA and are able to inhibit the CUGexp⋅MBNL1 interaction in cells that model DM1. In a DM1 cell model …
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Single-molecule study of the DNA helicase regulating genomic stability
… counteracting deleterious recombination. Using single molecule FRET technique and site-directed mutation, we discovered enzymes new activity: PcrA act as a powerful motor and remove RecA protein effectively from single strand DNA (ssDNA) as it pulls the RecA-coated lagging strand from the DNA …
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Monitoring the Effects of the Oncogenic S250F Mutation on the Intrinsically Disordered C-terminal of FoxA1
… containing the S250F mutation was purified and single-molecule FRET measurements conducted to monitor potential changes within the C-terminal, both free in solution and bound to DNA. The data obtained showed that the C-terminal on the mutated FoxA1 behaved differently than on the WT suggesting …
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Structural and Functional Effect of Phosphorylation On Ampa Receptors
… in the domain’s conformational landscape using single molecule FRET. Using wild-type GluA1 receptors, as well as glutamate and alanine to mimic phosphorylation and dephosphorylation respectively, we show based on FRET measurements that the C-terminal segment moves away from the membrane upon …
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Membrane protein conformational dynamics and ligand-binding interactions in bacterial glycoconjugate biosynthesis
… dynamics simulation (MD) in Chapter 3, and single-molecule FRET microscopy (smFRET) in Chapter 4 to observe the ligand-dependent conformational dynamics of integral membrane proteins in situ. It focuses on representative members of the small monotopic phosphoglycosyl transferase (SmPGT) …
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Single molecule studies of helicase mechanisms
… resolutions, as well as the ability to look at molecules one at a time instead of in an ensemble, have been shown to be helpful in elucidating the specific actions in many biological systems. Single molecule FRET (smFRET) microscopy provides these abilities with a distance scale of 2-8 nm, 8 ms …
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Studies of myosin mechanics using fluorescence
… Through fluorescence, individual biological molecules can be tracked as they interact with other proteins, DNA, ligands, etc. and change their conformation in response to the interaction. Molecular motors such as myosin provide an ideal system for fluorescence. The multitude of currently …
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Laser-assisted single-molecule refolding
… we have developed a new technique combining single-molecule FRET detection with laser induced temperature jump. We have calibrated the magnitude of the temperature jump with 1˚C accuracy using gold micro-size sensor. The accuracy of temperature calibration was confirmed by close agreement …
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Single Molecule Studies Of Spliceosomal Snrnas U2-U6
… different activation states. We have used single-molecule fluorescence to probe the</p> <p>structural dynamics of a protein-free RNA complex modeling U2-U6 from yeast and</p> <p>mutants of highly conserved regions of U2-U6. Our data show the presence of at least</p> <p>three distinct …
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