Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"single guide RNAs"”.
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In Situ Perturb-Seq of Transcriptomes and RNA Neural Recordings
… in the form of RNA, theoretically enabling single-cell resolution recording of whole-brain activity. This neural recording system can be coupled with perturb-seq in order to observe high-throughput genetic perturbations of neurons with both temporal and transcriptomic information. Untargeted …
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Cas9-induced on-target genomic damage
… on-target lesions induced by Cas9 complexed with single gRNAs and no exogenous template. I have followed the time dynamics of Cas9-induced small indels as a function of reagent delivery methods, established an assay for quantification of Cas9-induced genomic lesions that are not small indels …
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Regulation of LFA-1 on T cells by phosphoinositide signalling
… and designed a retroviral library of CRISPR/single guide RNAs targeting all known and potential PIP3-binding proteins. Using this library, I screened the targeted genes for effects on ICAM-1-binding by a flow cytometry-based ICAM-1-binding assay, using Cas9-expressing primary mouse T cells. I …
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Prevention of Duchenne Muscular Dystrophy by CRISPR/Cas Therapeutic Genome Editing
… by packaging Cas9 nuclease in conventional single-stranded AAV and CRISPR single guide RNAs in double-stranded self-complementary AAV. This strategy significantly reduces the amount of AAV vector needed for therapeutic genome editing and enhances dystrophin restoration after delivery into a …
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Innovations in Programmable Nucleic Acid Libraries and CRISPR Enrichment for Molecular Biology Applications
… these barcodes with in vitro transcribed sgRNAs, we successfully enriched three targeted barcodes by up to 1,094-fold. However, BAR-CAT scalability was limited beyond 12 targeted barcodes due to challenges with excessive library diversity and competition among sgRNAs for dCas9 binding, which …