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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"single chain fragment"”.
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Immunomodulation der Kartoffel-Blattrollvirus (PLRV) Multiplikation via Expression virusspezifischer rekombinanter Antikörper in planta
… terminus of P1 was cloned in order to obtain a single chain variable fragment scFvP1-C25 that was subsequently analysed. The resulting antibody was bacterially expressed in the single chain format and designated as scFvP1-C25. The engineered scFvP1-C25 displayed identical binding specificity of …
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Identifikation und Analyse differentiell exprimierter Proteine und Gene während der Thymozytenentwicklung
… <br>Die Analyse monoklonaler scFv-Phagen (single chain fragment variable) zeigte ein diverses <br>Repertoire, dessen Antikörperfragmente in durchflußzytometrischen Analysen zum Teil <br>differentiell exprimierte Antigene auf murinen Thymozyten und peripheren T-Zellen erkannten. <br>Western …
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Validation of an aASPH-SNAP-tag fusion protein for immunodiagnosis of triple-negative breast cancer
… of antibody derivatives which includes single chain fragments of variability (scFv) where a heavy chain variable region of a full-length antibody would have been recombinantly fused to its light chain variable region. The SNAP-tag is commonly known as the engineered version of the human …
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Structural and functional role of the extracellular loops and C-terminal domain of the voltage-gated sodium ion channel
… using an in-house phage display library of single chain fragment variable (scFv) antibodies, specific binders to the CTD of Nav1.5 and Nav1.7, were found and purified. These scFvs could have gating effects on their Nav channel targets, which might prove therapeutically applicable.
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INVESTIGATION OF LIGAND SIZE ON TARGETED PLGA NANOPARTICLES FOR HER2 BREAST CANCER: TRASTUZUMAB AND ITS ScFv ANTI-HER2 ANTIBODY
… antibody moieties, either whole IgG (TrAb) or single chain fragment variable (ScFv), were decorated on the PLGA NPs surface and evaluated in terms of their ability to target HER2 breast cancer cells. We observed the effects of these NPs against different cell lines (MCF-7 and SK-BR-3). Thus, …
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Konstruktion und Charakterisierung von rekombinanten Immuntoxinen zur Therapie von metastasierendem Pankreaskarzinom
… generated by genetically fusing the anti-EGFR single chain fragment 425(scFv) to a truncated mutant of Pseudomonas exotoxin A (ETA’). Using the expression vector pBM1.1, functional 425(scFv)-ETA’ was periplasmically expressed under osmotic stress conditions in the presence of compatible …
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Generation of recombinant SNAP-tag based antibody fusion proteins for immunotherapy of acute myeloid leukemia (AML)
… treatment of AML. Recombinant ADCs comprised of single-chain fragment variables (scFv) fused to a self labelling human protein called SNAP-tag were generated. SNAP-tag was used for site-specific conjugation to O6 -benzylguanine (BG) derivatives to demonstrate functionality against overexpressed …
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Entwicklung, Optimierung und Charakterisierung anti-CD64-basierter Immuntherapeutika
… targeted efficiently by the humanized anti-CD64 single chain antibody fragment (scFv) h22(scFv) and killed by ETA. However, the major drawback of such ITs is their potential immunogenicity. Repeated doses may cause hypersensitive reactions and lead to neutralization of the ITs by antibodies …
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Engineering human single-chain T cell receptors
… development of engineering strategies for human single-chain T cell receptor variable fragments (scTv), with the goal of understanding the properties that allow scTv to be expressed and deployed in a therapeutic mode. Stable scTv receptors can be used to generate high-affinity TCRs specific for …