Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"single cell imaging"”.
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Quantitative Single-Cell Imaging Reveals Insulation of Morphogenic Signal Transduction
How cells integrate external cues in order to make behavioral decisions is a central problem of cell biology. In development and in tissue-homeostasis, cell-fate decisions are made by the integration of multiple morphogenic signals, but how cells convert such combinations of signals into distinct …
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Microfluidics and live imaging advances: applications in host/pathogen, immunity and stem cell single cell phenotyping
Live single-cell imaging has emerged as an advanced single-cell study tool for approaching a quantitative understanding of many biological questions in recent years. In previous cell studies using bulk cell measurements, the population averages can miss the information from cell to cell variability …
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Integrative Analysis of the Myc and E2F pathway Reveal the Roles for microRNAs in Cell Fate Control
… pathways that are critical for making key cell fate decisions in normal cells. Understanding how these pathways operate under normal circumstances, therefore, is crucial for comprehensive understanding of tumorigenic process. With Myc and E2F pathways being central components for …
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Modeling Genome Organization in Eukaryotic and Prokaryotic Cells Using Folding Determinant Interactions
… to gene regulation, DNA replication, and cell identity. A key challenge, however, lies in disentangling stochastic folding driven by polymer physics from specific interactions mediated by nuclear landmarks and regulatory factors. In this thesis, I develop polymer-based chain-growth Monte …
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Functional Genomic and Image-Based Screening Approaches for Probing Host-Pathogen Interactions
… between bacteria or viruses and human cells, and the resulting evolved antipathogenic signaling pathways, are processes responsible for pathologies ranging from infectious diseases to autoimmune conditions and cancer. In addition, engineered designs inspired by pathogen interactions …
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Investigating luteinizing hormone receptor signal transduction using the cyclic AMP reporter ICUE1
… monitoring of cAMP levels in individual cells. In this project, Epac reporters have been used to evaluate LH receptor activity in cells expressing constitutively-active LH receptors or cells where transactivation of LH receptors is reported to occur. For the investigation of …
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Integrated Study Of Dinoflagellate Diversity In The Gulf of Naples
… many species cannot be cultured. I applied single cell imaging, PCR and sequencing to gather 18S and 28S for 22 Tripos species. Using 18S V4 region as barcode I assessed the seasonal abundances of these species. Some were common all year whereas others showed distinct seasonality, mainly …
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Measuring ongoing chromosomal instability in single-cell DNA sequencing data
… – a computational tool to correctly estimate single-cell ploidy and replication status. Cancer cells often exhibit DNA copy number aberrations and can vary widely in their ploidy as a consequence. Correct estimation of the ploidy of single cell genomes is crucial for many aspects of downstream …
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Robust spectral representations and model inference for biological dynamics
Current developments in automated experimental imaging allow for high-resolution tracking across various scales, from whole animal behavior to single-cell dynamics to spatiotemporal gene expression. Transforming these high-dimensional data into effective low-dimensional models is an essential …
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Behavioral Strategies and Neural Mechanisms of Dynamic Foraging
… behavioral modeling, large-scale widefield and single-cell imaging technologies, together with encoding models of neural responses, we showed and quantified the rich behavioral repertoire of rodents, state-dependent cortical processing of trial outcome, and diverse, distributed encoding of value …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
… defining features of mouse embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued …
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Investigating the role of TRPC3 in the biogenesis of extracellular vesicles
Cells within multicellular organisms transcribe, package and release nano-sized spheres called extracellular vesicles (EVs) that carry an array of biomolecules that can be transferred between cells. EV-mediated communication contributes to a wide range of physiological and pathological processes …
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Mass spectrometry imaging for probing molecular distributions in the nervous system
… from Aplysia californica. Mass spectrometry imaging (MSI) is a technique that allows for mass measurement of analytes, while also providing their spatial location and does not require a priori knowledge of the analytes to be imaged. MSI is capable of generating ion distribution images of many …
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Ionic Control of Porin Permeability
… molecules, including antibiotics, to enter the cell. Electrophysiological studies on porins reconstituted in lipid bilayers have suggested a dynamic regulation of porin conductance through changes in the ionic environment. Thus, this thesis focuses on understanding porin permeability regulation …
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Structural and Functional Effect of Phosphorylation On Ampa Receptors
… in the domain’s conformational landscape using single molecule FRET. Using wild-type GluA1 receptors, as well as glutamate and alanine to mimic phosphorylation and dephosphorylation respectively, we show based on FRET measurements that the C-terminal segment moves away from the membrane upon …