Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"single cell PCR"”.
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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS
… 23 hit compounds that are specific to mESC. The cell panel test identified CDy9 as the most specific compound. FACS sorting of CDy9 treated co culture of mESC and MEF proved the ability of CDy9 to isolate out mESC. Single cell PCR data, showed that CDy9 bright populations of cells were positive …
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Studying the Patterning Mechanisms and Cell Fates during Limb Regeneration in Ambystoma mexicanum
We studied patterning mechanisms and cell fates during limb regeneration in the axolotl. 1) It is crucial to understand the earliest events of patterning. Since it is technically challenging to study early events, we established single cell PCR. This new tool will allow us to obtain novel insight …
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Etablierung einer Fluoreszenz-Multiplex-Polymerase-Ketten-Reaktion zur semiquantitativen und qualitativen Analyse des V H-Repertoires in humanen B-Lymphozyten
Mit der VH-Fluoreszenz-Multiplex-PCR steht ein neuartiges Screeningverfahren zur semiquantitativen und qualitativen Analyse der VH-Familienverteilung der schweren Kette des Immunglobulinmoleküls in humanen B-Lymphozyten zur Verfügung. In zwei Multiplex-PCRs werden IgH Produkte der sechs häufigsten …
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Receptor-mediated calcium entry in retinal amacrine cells
In the vertebrate retina, multiple cell types express g protein-coupled receptors linked to phospholipase C. The signaling pathway engendered by activation of this enzyme can involve Ca<sup>2+</sup>-permeable transient receptor potential (TRP) channels. To begin to understand the role of these …
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Microfluidics for Cell Manipulation and Analysis
… controlled handling of the flow profile and the cells in suspension. Implementation of additional forces in the system, such as an electric field, promote further manipulation of the cells. In this dissertation, I show novel, unique microfluidic approaches for manipulation and analysis of …
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Mating interactions of the harmful dinoflagellate alexandrium tamarense from UK coastal waters
… of inoculum of motile vegetative A. tamarense cells in temperate zones during subsequent spring/summer blooms. This study provides further insight into the mating interactions between toxic Group I and non-toxic Group III isolates primarily from UK coastal waters. Study of the effect of …
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Quantification of gene expression in single cells
Studies of apparently homogeneous cell populations and single cells often give highly divergent results. Cells exhibit varying responsiveness to stimuli and gene expression and they are in many aspects stochastic and unpredictable. We have developed a method to measure gene expression …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… the normal development of male germ cells is controlled by both protein-coding genes and small non-coding RNAs. Because of the lack of in vitro models, mouse models are currently the most powerful tools used to study these processes.KLHL10 (Kelch-like 10) is a spermatid-specific …