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Showing 1 to 10 of 10 for “"single cell DNA sequencing"”.
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Measuring ongoing chromosomal instability in single-cell DNA sequencing data
… – a computational tool to correctly estimate single-cell ploidy and replication status. Cancer cells often exhibit DNA copy number aberrations and can vary widely in their ploidy as a consequence. Correct estimation of the ploidy of single cell genomes is crucial for many aspects of downstream …
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Investigating Metastatic Lineage In Colorectal Cancer By Single Cell Dna Sequencing
… have applied conventional bulk next-generation sequencing (NGS) methods, which have limited ability to resolve intratumor heterogeneity.</p> <p>To address this problem, we have developed a highly-multiplexed single cell DNA sequencing method that combines flow-sorting of single nuclei, …
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Statistical Methods For Resolving Intratumor Heterogeneity With Single-Cell Dna Sequencing
<p>Tumor cells have heterogeneous genotypes, which drives progression and treatment resistance. Such genetic intratumor heterogeneity plays a role in the process of clonal evolution that underlies tumor progression and treatment resistance. Single-cell DNA sequencing is a promising experimental …
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Reconstructing Mutational Lineages In Breast Cancer By Multi-Patient-Targeted Single Cell Dna Sequencing
… known regarding the timing and impact of single nucleotide variants (SNVs) contributing to these early transformative genomic events. Paramount to novel treatment options is understanding the underlying biology of initiation in the early stages of TNBC development, where inferring clonal …
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Investigating Invasion In Ductal Carcinoma In Situ With to pographical Single Cell Genome Sequencing
… these challenges, we developed Topographic Single Cell Sequencing (TSCS), which combines laser-catapulting with single cell DNA sequencing to measure genomic copy number profiles from single tumor cells while preserving their spatial context. We applied TSCS to sequence 1,293 single cells …
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Decoding Copy Number Substructure and Evolution From Single Cell Genomics
… However, these observations rely on limited cell numbers and inherent experimental bias from first-generation single cell technologies. Therefore, the evolutionary trajectory after the punctuated burst remains unknown. To address this question, we sequenced 9,765 cells from 8 primary TNBCs …
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Investigating The Rare Aneuploid Cells in Normal Breast and Therapeutic Resistance in Triple Negative Breast Cancer Using Single Cell Genomics
<p>Aneuploid epithelial cells are common in breast cancer, however their presence in normal breast tissues is not well understood. To address this question, we applied single cell DNA sequencing to profile copy number alterations (CNAs) in 83,206 epithelial cells from breast tissues of 49 healthy …
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Investigating The Ecology and Evolution of Normal Breast Tissues and Breast Cancer With Single Cell Genomics
<p>There is vast cellular heterogeneity in human breast tissues, with different transcriptional programs in the stromal, epithelial, and immune components, however, it remains unclear how their reprogramming and interplay leads to the progression of invasive phenotypes such as Triple- Negative …
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Quantitative Analyses of Normal and Precancerous Somatic Evolution in Human Tissues
Cancer arises from a single cell of origin whose lineage accumulates somatic mutations in a step-wise manner over time. The evolutionary process towards cancer development is dynamic and the earliest mutation may arise decades before the onset for some cancers. This calls for a quantitative …
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Orthogonal Comparison of Nuclear and Mitochondrial Clonal Architectures in Hematologic Malignancies
… panels) or low throughput and high cost (in single-cell whole genome sequencing, scWGS). An emerging alternative is the use of mitochondrial DNA (mtDNA) mutations as clonal markers.</p> <p>This study aims to determine whether mitochondrial-derived clonal architectures correlate with …