Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"signal recognition particle"”.
-
Biochemical and genetic analysis of a fission yeast ribonucleoprotein homologous to signal recognition particle
… on a ribonucleoprotein complex known as signal recognition particle (SRP). SRP is composed of six polypeptides and one molecule of 7SL RNA. A homolog of mammalian SRP RNA was found in the fission yeast, Schizosaccharomyces pombe. The gene encoding the S. pombe RNA was cloned by screening …
-
Analysis of essential structural and functional elements in the GTP-regulated signal recognition particle cycle
I have investigated signal recognition particle (SRP)-mediated protein targeting using a combination of genetic, biochemical, and molecular sequence analysis techniques. First, I consider the SRP cycle from the perspective of molecular evolution. This analysis provides insight into the significance …
-
The Importance of RNA Sequence and Structure in the Assembly and Function of Signal Recognition Particle
Signal recognition particle (SRP), the ribonucleoprotein responsible for the recognition and targeting of nascent secretory proteins to the endoplasmic reticulum, is composed of 7SL RNA, along with six polypeptides. Earlier work in our lab identified a homolog of 7SL RNA in S. pombe, showed by gene …
-
Genetic analysis of Schizosaccharomyces pombe 7SL RNA
Signal recognition particle (SRP), a ribonucleoprotein composed of six polypeptides and one molecule of 7SL RNA, is required for translocation of secretory proteins into mammalian microsomal vesicles in vitro. In order to investigate the function of the RNA component of SRP, mutations were …
-
Unterschiedliche Funktionen von SRP und SecA beim Transport eines bakteriellen Proteins
… während ihrer Synthese am Ribosom anhand ihrer Signal-Anker-Sequenzen vom Signal recognition particle (SRP) erkannt; ihre Zielsteuerung und Integration in die Innenmembran erfolgen kotranslational und werden von SRP und seinem Rezeptor FtsY vermittelt. Sekretorische Proteine (lösliche …
-
RNA Localization and Translational Regulation on the Endoplasmic Reticulum
… cell fate specification, and synapse formation. Signal sequence-encoding mRNAs are localized to the endoplasmic reticulum (ER) membrane by either of two mechanisms, a canonical mechanism of translation on ER-bound ribosomes (signal recognition particle pathway), or a poorly understood direct ER …
-
Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
… amino terminal extension sequence known as the signal peptide. Signal peptides contain a central core of uncharged amino acids that are usually hydrophobic in nature. The hydrophobic core of the parathyroid hormone (PTH) signal peptide is composed of 12 contiguous hydrophobic amino acids. …
-
In vitro Untersuchungen zum Translationsarrest in Escherichia coli
… in der Säugetierzelle wahrnimmt, ist der Signal Recognition Particle (SRP). Dieser bindet an die Signalsequenz gerade entstehender Proteine, unterbricht deren Translation und steuert das Ribosom mit der naszierenden Proteinkette zur Membran des Endoplasmatischen Retikulums (ER). Die …
-
In vitro-Untersuchungen zum Sekretionsmechanismus der beta-Lactamase in Escherichia coli
… in Abhängigkeit von SecA. <br>2. Das bakterielle signal recognition particle (SRP) und sein Rezeptor FtsY vermitteln die kotranslationale Zielsteuerung und Integration polytoper Innenmembranproteine an bzw. in das SecYEG-Translokon. <br>Nicht alle Innenmembranproteine sind jedoch ausschließlich …
-
Structural Characterisation of the Mammalian SRP Receptor
… This requires the specific interaction of the signal recognition particle (SRP), a ribonucleoprotein, with its receptor (SR). The eukaryotic SR is a heterodimeric protein consisting of SRα and SRβ which is anchored to the ER membrane. In all three kingdoms of life the conserved GTPases in SRP …
-
In vitro studies on the SecA involvement in the assembly of an integral membrane protein with large hydrophilic domain
… proteins are co-translationally targeted by signal recognition particle (SRP) and its receptor FtsY, while secretory proteins follow a post-translational targeting mode mediated by SecB/SecA. The subsequent transport, of secretory proteins across the membrane depends on the ATPase activity of …
-
Untersuchungen zum Integrations- und Translokationsmodus des Sec-Translokons in Escherichia coli
… erfordert in Pro- wie in Eukaryoten eine signal recognition particle (SRP) vermittelte Zielsteuerung und die Integrase-Aktivitaet des Translokons. Bakterielle Innenmembranproteine mit ausgedehnten periplasmatischen Domaenen wie das Hybridprotein Momp2 benoetigen eine koordinierte Aktion …
-
In vitro-Untersuchungen zum Sekretionsmechanismus von Pathogenitätsfaktoren mit einer N-terminalen Extension in Escherichia coli
… von SecA energetisiert. <br>2. Das bakterielle signal recognition particle (SRP) und sein Rezeptor FtsY vermitteln sowohl die co-translationale Zielsteuerung polytoper Innenmembranproteine an, als auch ihre Integration in das SecYEG-Translokon und die Innenmembran. <br>Nicht alle …
-
Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… inserted into the cytoplasmic membrane contain a signal sequence at their N-terminus that is recognized by and targeted to the translocation machinery. Three translocation pathways have been described, so far in E. coli to allow secretion of proteins: The Sec, the Tat and the SRP (Signal …
-
Molecular dissection of competence regulated virulence in Streptococcus pneumoniae
… acute pneumonia to sepsis, and the competence signal kept increasing until death. For mice with more prolonged course of the disease, the naturally-developed competent state might wane, but would increase again when the infection entered the sepsis state and lasted for >40 hours until the …