Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 99 for “"signal peptide"”.
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Tat signal peptide recognition during protein maturation and export
… terminal reductase NapA has an N-terminal signal peptide harbouring a Tat (twin-arginine translocation) motif, which follows closely the consensus S/T-R-R-x-F-L-K. As with other proteins transported via the Tat pathway, NapA needs to be fully folded, and cofactor insertion needs to be …
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Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
… amino terminal extension sequence known as the signal peptide. Signal peptides contain a central core of uncharged amino acids that are usually hydrophobic in nature. The hydrophobic core of the parathyroid hormone (PTH) signal peptide is composed of 12 contiguous hydrophobic amino acids. …
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Characterization of the Glycosylation and Signal Peptide Sequences Within the Vaccinia Virus M2 Protein and Influence on ERK1/2 and Nf-Kappab Activation
… protein, and its impact on cytoplasmic signaling pathways within the context of an anti-viral immune response.
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Characterization of the glycosylation and signal peptide sequences within the vaccinia virus m2 protein and influence on ERK1/2 and NF-kB activation
… M2 amino acid sequence revealed an N-terminal signal peptide sequence, 4 putative glycosylation residues, and endoplasmic reticulum (ER) retention/retrieval sequences, indicative of an ER-localized protein. Confocal microscopy analysis confirmed localization of M2 to the ER. It was found that …
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Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
Protein engineering systems have been developed which simplify the cloning, oligonucleotide directed mutagenesis, enzymatic sequence analysis, and expression of mutant proteins, all from a single vector. Chimeric phage-plasmid expression vectors, or phagemids, containing the intergenic region of …
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Interactions between the TatBC complex and Tat signal peptides during protein transport by the bacterial Tat pathway
… are targeted to the Tat machinery via N-terminal signal peptides that have a tripartite structure, with a polar n-region, a hydrophobic h-region and a polar c-region. They critically contain an almost invariant twin-arginine (RR) motif within the n-region that is essential to trigger Tat …
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Probing the role of Tat proofreading chaperones in the assembly of molybdoenzymes
… targeted to this system by N-terminal signal peptides which bear a conserved SRRxFLK motif. Tat dependent proteins often contain redox factors that must be inserted prior to translocation. The E. coli Tat substrates trimethylamine Noxide<br/>reductase (Tor) and periplasmic nitrate …
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ROLE OF HEPATIC APOLIPOPROTEIN M AND ATP-BINDING CASSETTE TRANSPORTER A1 (ABCA1) IN LIPID METABOLISM
… ApoM binds to plasma HDL via its retained signal peptide and transports sphingosine 1–phosphate (S1P). We found that liver–specific apoM transgenic mice had larger plasma HDLs enriched with apoM, cholesteryl ester, lecithin:cholesterol acyltransferase and S1P, but not enhanced …
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Investigations into the roles of bacterial TorD family chaperone proteins
… system are targeted to the system by N-terminal signal peptides, which bear the consensus motif (S/T)RRxFLK. These proteins often require redox centres for function that must be incorporated prior to transport, with many utilising the molybdenum cofactor Mo-bis-MGD for enzymatic activity during …
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Investigating the Unique Mechanism of Action of CADA Analogs for Down-Modulating CD4, Sortilin, and ACE2
… glutamine (Q-15) and proline (P-20) in the hCD4 signal peptide (SP). It has been proposed that CK147 may bind with the hCD4 signal peptide through a dipole-dipole interaction between the N,N-dimethylaminobenzenesulfonyl side-arm of CK147 and the amide side chain of glutamine (Q-15) of hCD4 SP, …
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Apolipoprotein M – Studies of Structure and Function
… Mature apoM is circulating with a retained signal peptide. By constructing apoM with a cleavable signal peptide we were able to show that the signal peptide is necessary for the protein’s ability to associate to lipoproteins. We were also able to show that, in contrast to apoM with a …
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Modification of a plant-based expression system to produce Lysosomal Acid Lipase (LAL) in plant cells using genetic modification of endogenous plant N-glycosylation
… fusion of a sialyltransferase-derived N-terminal signal peptide is capable of targeting B4GALT1 to the medial/trans-Golgi. As a proof of concept for human therapeutic enzyme production in plant cells, Lysosomal Acid Lipase (LAL) was expressed in tobacco leaf cells. Here LAL was shown to localise …
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Molecular characterization and induction of a peach beta-1,3-glucanase gene
… amino acids, which consists of a 38 amino acid signal peptide and a 312 amino acid mature peptide. This is the longest signal peptide of all plant $\beta$-1,3-glucanase genes so far reported. Putative TATA and CAAT boxes in the 5$\prime$ flanking region and two polyadenylation signals in the …
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Archaeal signal peptides and cell surface structures
… unfold. Integral to this process are the various signal peptidases. Two types of archaeal signal peptidases are identified thus far: signal peptidase I (SPI) and the preflagellin peptidase. SPI is responsible for processing the majority of secreted proteins. Sequence analysis of archaeal SPI …
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Proteoliziniu aktyvumu pasižyminčių fermentų biosintezė /
… KER78, and to synthesize KER78 without a signal peptide. For KER910 and KER1314 without a signal sequence, 1 mM inducer (IPTG) should be used. From the results, we can conclude that the synthesis of KER78 recombinant protein without a signal peptide begins 2 hours after induction, while …
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Molecular and Pharmacological Characterization of the Rat RAMP2B Splice Variant
… calcitonin receptor (CTR), vasoactive intestinal peptide/pituitary adenylate cyclase activating peptide (VPAC) receptor 1, parathyroid hormone (PTH) receptor 1 and glucagon receptor. An adrenomedullin receptor (AM2) is also formed by the RAMP3 and CL complex. A partial sequence of a rat RAMP2 …
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"Characterization of an antifreeze glycopeptide gene from the Antarctic cod, Notothenia neglecta, and a functional study of the ""pre-"" sequence of this antifreeze glycopeptide"
… a series of 8 different-sized antifreeze glycopeptides (AFGP 1 to 8) which lower the freezing point of its body fluids below that of sea water. I report here the sequence of one of its AFGP genes. The structural gene contains 46 tandemly repeated segments, each coding for one mature peptide. …
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The prlC Suppressor Phenotype and Its Relation to Oligopeptidase a Specificity
… the PrlC mutant proteins suppress many different signal peptide mutations with no changes in the active site of OpdA, it is likely that wildtype OpdA also recognizes substrates of different lengths and sequences.
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Genetic and biochemical analysis of the colicin I receptor gene of Escherichia coli: Structural organization and transcriptional control mechanisms
… The Cir protein was processed by removal of a signal peptide and the mature form had an amino terminal sequence which closely resembled that of other TonB-dependent proteins.
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Biological Functions of Intracellular Hepatitis B e Antigen
… intermediate (p22) without the N-terminal signal peptide, remains elusive. Here, we propose to elucidate the translocation of p22 during its formation from endoplasmic reticulum (ER) to cytosol, how it differs from core in its inability to form a capsid and the biological functions of …
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