Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"sialylation"”.
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High-throughput quantification of glycoprotein sialylation
… develop a novel HTM for quantifying glycoprotein sialylation. Using chemical reduction, enzymatic release of sialic acid, and chemical derivatization, the HTM can accurately, rapidly (15 min), and specifically analyze many samples in parallel. It requires only 45 piL of sample and has a …
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Engineering mammalian cell line to improve sialylation
One of the key problems faced by many biotechnology companies is the cleavage of terminal sialic acid on the glycans of the therapeutic glycoproteins. This is caused by the degradative action of sialidase released to supernatant when the cell starts to die. This phenomenon is undesirable because …
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Sialylation as a mechanism for preferential Ligand binding in CFS1R
… also exhibits α2- 8 polysialic acid chains. Polysialylation has been previously associated with immunomodulation, but I have found that treating cells with an enzyme that can remove poly sialic acid potentiates proliferation of cells in a ligand dependent manner. This provides a new mechanism for …
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Investigation of Haemophilus somnus Virulence Factors: Lipooligosaccharide Sialylation and Inhibition of Superoxide Anion Production
… phase variation of these LOS epitopes. Sialylation of the terminal galactose of H. somnus LOS is another reported virulence mechanism. Current sequencing of the genomes of H. somnus strains 2336 (pathogenic) and 129Pt (commensal) has enabled in silico identification of three open reading …
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Effect of Sialylation of Histophilus somni Lipooligosaccharide on Virulence and Resistance to Host Defenses
… In the present study, we assessed the effect of sialylation of Histophilus somni LOS on complement-mediated killing, binding of complement factor H (which converts C3b to inactive C3b (iC3b) and inhibit the alternative complement pathway) to the bacteria, complement activation by the LOS, and …
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Synthesis And Á-Stereoselective Sialylation Of Kdn And Late Stage Modification Of Neuraminic Acid Sialosides
… <p>SYNTHESIS AND á-STEREOSELECTIVE SIALYLATION OF KDN AND LATE STAGE MODIFICATIONS OF NEURAMINIC ACID SIALOSIDES</p> <p>by</p> <p>CHANDRA SEKHAR NAVULURI</p> <p>December 2012</p> <p>Advisor: Prof. David Crich</p> <p>Major: Chemistry</p> <p>Degree: Doctor of Philosophy</p> <p>Keto …
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The role of n-linked glycosylation and sialylation in regulating human proteinase-activated receptor 1 and 4 expression and signalling
Proteinase-activated receptors (PARs) are a novel family of G-protein coupled receptors (GPCRs) that mediate many of the diverse biological effects of proteinases on target cells. N-linked glycosylation is a common post-translational modification in the GPCR superfamily that enables the cell to …
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New Methodology for Alpha-Sialyl Glycoside Synthesis
… excellent alpha-selectivity under a variety of sialylation protocols. This C1-auxiliary has also been used in the development of the dehydrative sialylation, employing the reagent combination of (p-nitrophenyl)(phenyl) sulfoxide and trifluoromethane-sulfonic anhydride. The dehydrative …
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Characterization and improvement of interferon-[gamma] glycosylation in Chinese hamster ovary cell culture
… A critical aspect of glycosylation is sialylation since the presence of sialic acid can dramatically extend a glycoprotein's circulatory lifetime. The sialylation patterns of CHO-derived IFNY-[gamma] were characterized by RP-HPLC separations of glycosylation site-specific tryptic …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… significantly different capabilities in N-glycan sialylation, which follows the order: (BHK-21, CHO) > (COS-7, HEK293) > 3T3. Significant difference in O-glycosylation was also detected: CHO-K1 was found to produce only mono- or di-sialyl T antigen structures whereas the other four cell lines were …
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Sex-specific gene expression and its effect on osteoclast maturation
… This sugar is later used as a substrate for sialylation. Our analysis found that terminal α2-3 sialylation is necessary for proper osteoclast differentiation and morphology, but removal of these specific sialic acids does not appear to more strongly impact one sex over the other. In this …
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Molecular Responses to Desialylation in a Rat Model of Induced Glomerulopathy
… functional glomerular damage, results in a re-sialylation event associated with sequentially increased levels of glomerular linkage-specific sialyltransferase transcript levels and activity. The results of these experiments provide insight into specific molecular responses of podocytes to …
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Convergent Site-Selective Carbohydrate-Peptide Ligations With Dehydroalanine and Aziridine -2 -Carboxylic Acid-Containing Peptides
… and glycolamide-auxiliary controlled sialylation reactions. The efficiency of the dehydroalanine ligation approach was demonstrated by the preparation of thio-isosteres of the four tumor-associated carbohydrate antigens, T N, T, STN, and 2,6-ST, as a pair of diastereoisomers at the …
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The significance of cell-surface α2,3-linked Sialic Acid in Osteoclasts
… able to verify that there is widespread a2,3-sialylation of osteoclasts during their differentiation, however, we were unable to reproduce-in RAW 264.7 cells-the morphological changes previously seen in primary bone marrow macrophages following cellsurface a2,3-linked sialic acid ablation. …
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An investigation of the regulation of Sialoadhesin ligands on CD4+ T cells and its potential significance in Systemic Lupus Erythematosus
… Experiments performed to analyse the nature of sialylation changes and expression of glycosyltransferases on SnL+ CD4+ T cells, indicated that terminal sialic acids on Nlinked glycans represent a putative ligand for Sn.The second aim of this project was to analyse the role of Sn in a mouse model …
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Studies on the specificities of <i>In Vivo</i> and <i>In Vitro</i> bioassays used in the potency determination of Therapeutic Erythropoietin
… for rendering cell-based bioassays sensitive to sialylation. The strategies' common feature was the inclusion of a mechanism for depleting or sequestering desialylated Epo via a galactose-binding protein (derived from either plants or the human liver). The assays were tested usinga range of Epo …
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PROTEIN PURIFICATION FROM GASTROPODS
… O-glycosylation pattern with a focus on their sialylation and fucosylation abilities. Their sialylation potential was investigated by Sambucus nigra agglutinin and Maackia amurensis agglutinin while their fucosylation potential was investigated by Aleuria aurantia lectin, Lens culinaris …
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Production and characterisation of novel recombinant antibody fragments against sialic acid
… contain terminal sialic acids or altered sialylation patterns. The identification of sialic acid-specific antibodies is important in the fields of basic research and diagnostics. Therefore the primary objective of this thesis was the generation and characterisation of recombinant …
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Metabolic Pathways for Natural and Unnatural Sialic Acids
… binding to cells. Furthermore, I found that sialylation of N-glycans can further influence N-glycan branching. Taken together, my work suggests unexplored mechanisms for hIBM pathogenesis. The use of unnatural sialic acids, such as SiaDAz, and metabolic carbohydrate engineering is enabling …
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