Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"sialidase"”.
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Mechanisms and measurements of sialidase in pneumonia
Streptococcus pneumonia is a major human pathogen that resides in the upper airway and nasopharynx. It colonizes asymptomatically but can spread to other regions in the host body causing diseases such as bacteremia, otitis media and meningitis. This pathogen has evolved to evade the host-immune …
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Immunomodulatory Roles of the Lysosomal Sialidase Neuraminidase 1
… acids in mammalian cells is carried out by four sialidases, which are differentially expressed and localized in distinct subcellular compartments. Neuraminidase 1 (NEU1), the most abundant and ubiquitous of the four sialidases, functions primarily in the acidic environment of the lysosomes, but …
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Characterization of putative sialidase genes in Gardnerella vaginalis
… vaginalis in terms of virulence factors such as sialidase activity, may explain why it is also observed in asymptomatic women. This thesis aimed to identify genomic determinants of sialidase activity in G. vaginalis and better understand its role in BV. G. vaginalis has demonstrated genotypic and …
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The role of bacterial vaginosis-associated sialidase on HIV-1 infection
… vaginosis-associated bacteria (BVAB) release sialidases which alter either the virus and host cells directly or the viscosity of the female vaginal tract (FGT) mucosal barrier, increasing the likelihood of infection. This study aimed to determine the impact of bacterial sialidase on HIV-1 …
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Thymoquinone is a Novel Ligand which Activates Neu4 Sialidase to Promote a Pro-Inflammatory Response
… lipopolysaccharide (LPS)-induced cellular sialidase activity in an anti-inflammatory manner. Sialidase activity was tested on live mouse bone marrow derived primary macrophage cells, BMC-2 macrophage cells, human embryonic kidney epithelial (HEK293) cells and human fibroblast cells using an …
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Engineering mammalian cell line to improve sialylation
… This is caused by the degradative action of sialidase released to supernatant when the cell starts to die. This phenomenon is undesirable because the loss of terminal sialic acid results in a product which is rapidly removed from the plasma by the interaction with asialoglycoprotein receptors …
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Investigation of the role of the toxins perfringolysin O (PFO) and sialidase in Clostridium perfringens gas gangrene infections
… persistence. To investigate the role of sialidase in C. perfringens gas gangrene we made nanI-, nanJ-, and nanI-/nanJ- mutants. We observed that NanI is responsible for the majority of sialidase activity of C. perfringens strain 13, that NanJ is an extracellular sialidase, and that these …
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Studies on Virulence Factors of Clostridium perfringens and Necrotic Enteritis in Chickens
… hydrolases, clostripain, perfringolysin O, and sialidase. The study showed that sialidase was produced 455.1 fold higher by the pathogenic CP strain. Sialidase has been shown to be an important virulence factor of several human pathogens and it also been shown to have synergistic effects with CP …
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Role of Neuraminidase-mediated Desialylation in Microglial Activation
… down of Neu1 reduced the microglial surface sialidase activity induced by LPS, indicating that this enzyme may be responsible for surface desialylation of microglial cells. LPS- stimulation also encouraged release of a neuraminidase into the culture supernatants of microglia, which was …
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Measuring the effects of reaction coordinate and electronic treatments in the QM/MM reaction dynamics of Trypanosoma cruzi trans-sialidase
… reactions comprising the T. cruzi trans-sialidase catalytic itinerary. This enzyme was chosen for the wealth of experimental data available for it built from its significance as a potential drug target against Chagas disease. Of equal importance is the identification of an elimination …
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The effect of bacterial vaginosis on HIV infection
… virulence factors such as vaginolysin (VLY) and sialidase and are known to initiate the formation of biofilms and hence, contribute to the pathology associated with BV. As many studies have suggested that BV increases susceptibility to HIV infection, it stands to reason that Gardnerella spp. …
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Characterizing the genotypic and phenotypic diversity of Gardnerella vaginalis from vaginal clinical samples
… protective vaginal mucus through the release of sialidase. Recent evidence suggests that the G. vaginalis species is more heterogeneous that initially thought and that not all G. vaginalis may be involved BV. The aim of this study was thus to characterize the genotypic and phenotypic diversity of …
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A metagenomic approach to understanding the relationship between the vaginal microbiome and women’s reproductive health
… existing research on BV focused on the latter. Sialidase assists the colonization of pathogens by destroying mucin. Therefore, we hypothesized that sialidase could serve as a biomarker for BV (chapter 2). We used the BVBlue sialidase test to measure the sialidase activity, 454-pyrosequencing of …
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Development of Chemical Tools to Discover and Characterize Sialic Acid Mediated Interactions
… the challenges in studying their interactions. Sialidases are enzymes that hydrolytically cleave sialic acid from its underlying glycan. In this regard, sialidases have the potential to impact numerous cellular processes in a dynamic fashion. Relatively little is known about the substrates of …
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Cannabinoid Type 2 Receptors Promote Cadherin Switching While Suppressing Cell Migration: A Novel Investigation into Biased GPCR Signaling
… SER-601, and HU-308, significantly induce Neu1 sialidase activity in a dose-dependent fashion in RAW-Blue (murine macrophages) and PANC-1 (pancreatic) cells, as assessed by live-cell sialidase assay. This activity was abrogated by selective inhibitors of NMBR (BIM-23127), MMP9 (MMP9-i), and Neu1 …
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Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice
<p>The lysosomal sialidase Neuraminidase-1 (Neu1) initiates the hydrolysis of sialoglycoconjugates by cleaving their terminal sialic acid residues. Neu1 creates a complex with the carboxypeptidase protective protein/cathepsin A (PPCA), which is necessary for its catalytic activation and its …
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Untersuchungen zu Bacillus cereus Enterotoxin-Komplexen auf zellulärer Ebene
… castanospermine nor enzymatic treatment with sialidase and N-glycosidase F resulted in an alteration of toxicity.
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Metabolism of host-derived carbohydrates by Bifidobacterium breve UCC2003
… a prominent HMO, by the extracellular sialidase activity of Bifidobacterium bifidum PRL2010. Chapter III reports on the transcriptional regulation of sialic acid metabolism in B. breve UCC2003 by a transcriptional repressor encoded by the nanR gene. NanR belongs to the GntR-family of …
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Caffeine Binding Non-Canonical Biased Adenosine A₂AR GPCR Modulates Neu1 Activation and Signaling in Breast Cancer Cells
… phenotypes in breast cancer cells. Using sialidase assays, we found that caffeine induced Neu1 enzymatic activity in both MDA‑MB‑231 (TNBC) and MCF‑7 (luminal) cells in a dose‑dependent manner. This induction was abrogated by pharmacological inhibitors of NMBR (BIM‑23127), MMP9 (MMP9i), or …
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