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Showing 1 to 20 of 110 for “"sialic acid"”.
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Regulation of Sialic Acid Metabolism
In bacteria, sialic acids act as virulence factors for certain pathogens, and have been determined to play an important role in the establishment of many disease states. Therefore, studying the intricacies of sialic acid metabolism and its regulation provide a foundation for studying several …
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Molecular and Biochemical Characterisation of SiaP as a Sialic Acid Binding Protein Component of a TRAP Transporter of Sialic Acid.
Sialic acid utilisation plays an important role in the growth and persistence of the obligate human mucosal pathogen Haemophilus influenzae, which causes respiratory tract infections, septicaemia and meningitis. Like many other bacteria, H. influenzae can use host-derived sialic acids as carbon, …
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Production and characterisation of novel recombinant antibody fragments against sialic acid
Sialic acids are a family of acidic monosaccharides that typically reside as terminal moieties on N- and O-linked glycans. These sugars are actively involved in a plethora of biological phenomena, ranging from cell-cell adhesion and recognition, intracellular signalling events, pathogen attack, …
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The significance of cell-surface α2,3-linked Sialic Acid in Osteoclasts
… sought to determine the effect that a2,3-linked sialic acid on the surface of osteoclast precursors has on the formation or function of mature osteoclasts. Previous work by our lab lead, Dr. Jason W. Ashley, initially showed that a prevalent osteoclast biomarker, CD68, is heavily glycosylated …
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Development of Chemical Tools to Discover and Characterize Sialic Acid Mediated Interactions
… involve a class of carbohydrates known as sialic acids. The presence of sialic acid on a glycan can recruit specific sialic acid binding partners. Alternatively, sialic acids can mask underlying glycan epitopes, thereby preventing protein binding. There are several challenges in studying …
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A Systematic Study of Sialic-Acid-Containing Poly(styrene sulfonate) for Antiviral Applications
… by influenza viruses to initiate infection, sialic acid. Through systematic variation of polymer parameters, libraries of styrene-sulfonate sialic acid-containing copolymers with covaried degree of polymerization and sialic acid content were synthesized and evaluated for their inhibition of …
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EFFECT OF DIETARY GLYCOMACROPEPTIDE AND CHOLESTEROL ON CORTICAL GANGLIOSIDE- AND GLYCOPROTEIN-BOUND N-ACETYLNEURAMINIC ACID IN YOUNG RATS
Background: Sialic acid and cholesterol are present in human milk and accumulate rapidly in the brain during development. Infant formulas contain little sialic acid or cholesterol. Sialic acid and cholesterol supplementation increase cortical ganglioside and glycoprotein sialic acid and cholesterol …
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High-throughput quantification of glycoprotein sialylation
Sialic acid can improve qualities of therapeutic glycoproteins, such as circulatory half-life, biological activity, and solubility. In production of therapeutic glycoproteins, a high-throughput method (HTM) is required for process monitoring and optimization to ensure consistent and optimal sialic …
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Metabolic Pathways for Natural and Unnatural Sialic Acids
Carbohydrates, along with lipids, nucleic acids, and amino acids constitute the four main building blocks of life. This thesis will focus on sialic acid, a unique, nine-carbon sugar that is critically important in humans. In Chapter 1, I will introduce sialic acid biology and the many diseases that …
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Direct detection of homocysteine and syntheses of lanthanide complexes as biosensors
… In addition to the detection of bioactive amnino acids (such as Hcy and congeners), the detection of neutral sugars has been of great interest and ongoing for over a century. A big challenge remaining in this area is to achieve sensitive determination of specific saccharides at physiologically …
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Characterization and improvement of interferon-[gamma] glycosylation in Chinese hamster ovary cell culture
… is sialylation since the presence of sialic acid can dramatically extend a glycoprotein's circulatory lifetime. The sialylation patterns of CHO-derived IFNY-[gamma] were characterized by RP-HPLC separations of glycosylation site-specific tryptic glycopeptides. IFNY-[gamma] displayed …
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Engineering mammalian cell line to improve sialylation
… companies is the cleavage of terminal sialic acid on the glycans of the therapeutic glycoproteins. This is caused by the degradative action of sialidase released to supernatant when the cell starts to die. This phenomenon is undesirable because the loss of terminal sialic acid results …
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Xanthene/Benzoxanthene Dyes: Novel Synthetic Metholologies, Applications in Molecular Recognition, Cellular Imaging and the Design of a Tunable White (RGB) Light Emitter
… research problems. Selective detection of sialic acid via a boron-amide interaction. Concentration changes of sialic acid can be monitored ratiometrically in the visible region using a boronic acid based chemosensor. The technique shows great promise for the detection of sialic acid, a …
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Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice
… sialoglycoconjugates by cleaving their terminal sialic acid residues. Neu1 creates a complex with the carboxypeptidase protective protein/cathepsin A (PPCA), which is necessary for its catalytic activation and its transport to the lysosomal compartment. In humans, a primary deficiency of this …
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Lanthanide complexes as fluorescent indicators for neutral sugars and cancer biomarkers
… constants comparable to those of arylboronic acids. Interference from commonly occurring anions is minimal. The europium complex detects sialic acid-containing gangliosides at pH 7.0 over an asialoganglioside. This selectivity is attributed, in large part, to the cooperative complexation of …
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Metabolism of host-derived carbohydrates by Bifidobacterium breve UCC2003
… II describes B. breve UCC2003 utilisation of sialic acid, a nine-carbon monosaccharide, which is found in human milk oligosaccharides (HMOs) and the mucin glycoprotein. B. breve UCC2003 was also shown to cross-feed on sialic acid released from 3’ sialyllactose, a prominent HMO, by the …
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Isolation of a human milk sialyloligosaccharide by affinity chromatography with wheat germ agglutinin (WGA)
… subjected to neuraminidase digestion to remove sialic acid, and the resulting neutral oligosaccharide had more affinity for the WGA-agarose column. Sequential exoglycosidase digestion of the asialo derivative of the WGA-bound fraction with jack bean β -galactosidase and β -hexosaminidase …
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