Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 148 for “"siRNAs"”.
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Chemische Modifikation von siRNAs zur Verbesserung der zellulären Aufnahme
… zielte auf die Erhöhung der Hydrophobizität der siRNAs und eine damit verbundene Erleichterung der zellulären Annäherung. Als Grundbausteine dienten Lithocholsäure, Cholesterin, Betulin und 3-Beta-Cholestanol. Durch kurze organisch chemische Synthesesequenzen wurden diese Verbindungen in das …
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Drug Substance and Drug Product Manufacturing Strategy Assessment for siRNAs
Amgen currently has its first siRNA program, Asset #1 in phase 2 clinical trials. Until recently, Amgen has been outsourcing the Drug Substance (DS) and Drug Product (DP) manufacturing to external manufacturers, but with a growing siRNA portfolio even beyond Asset #1, the building of a new facility …
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The conjugation and evaluation of hydrophobic small molecules to trazole-linked siRNAs
… therapies, such as short interfering RNAs (siRNAs), have been developed to aid in the regulation of proteins. SiRNAs serve as substrates for the highly conserved RNA interference (RNAi) pathway, which gives rise to their high degree of mRNA target specificity and their catalytic nature. …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… Y-branch and hyperbranch GRP78-silencing siRNAs. The novel V-shape, Y-branch and <strong> </strong> hyperbranch motifs were then studied by CD spectroscopy and thermal denaturation experiments. CD spectroscopy was used to characterize the requisite A-type double-stranded RNA helix for RNAi …
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Synthesis, characterization and biological investigation of self-delivering and modified short interfering RNAs (siRNAs)
… mRNA. Synthetic short interfering RNAs (siRNAs) can use this endogenous machinery and have emerged as a novel class of gene-silencing therapeutics. Unfortunately, the development of RNAi therapeutics has been hindered by several challenges associated with the nature and structure of RNA. …
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Discovery of IKBKE and CD24 siRNAs for the treatment of triple-negative breast cancer
… the stability and improve the cellular uptake of siRNAs. This dissertation focuses on two primary research objectives. The first objective centered on discovering siRNAs targeting IKBKE and CD24 for the treatment of TNBC. The second research objective aims to utilize a recently discovered …
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Synthesis of internal amide bond short interfering RNAs (siRNAs) and investigation of their gene silencing properties
… therapeutics are short interfering RNAs (siRNAs). These siRNAs serve as the effectors of RNA interference, a naturally occurring and highly specific mechanism for regulating gene expression through sequence-specific degradation of messenger RNA. However, the native structure of RNA is …
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The synthesis and in vitro evaluation of chemically modified siRNAs that contain internal azobenzene derivative spacers for selective and tunable photocontrol of activity
Short interfering RNAs (siRNAs) are biopolymers that are used as post-transcriptional gene regulators and as a natural endogenous defense against attack from viruses. These molecules hold a great deal of interest as pharmaceuticals for the knockdown of unregulated genes, such as in cancers. Through …
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Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing
… a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling their role in the RNAi pathway. An Ortho-functionalized, …
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Structural variation at soybean loci regulating small RNAs and seed color
… of small RNAs known as short- interfering RNAs (siRNAs). The dominant ii allele of the I (inhibitor) locus is composed of an inverted-repeat cluster of six chalcone synthase (CHS) genes on chromosome 8 whose unique arrangement generates CHS siRNAs that downregulate target CHS7 and CHS8 genes on …
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Endogenous short interfering RNAs that silence chalcone synthase result in pigment pattern formation on soybean seed coats
… (CHS) genes leading to production of CHS siRNAs which in turn degrade CHS mRNAs resulting in yellow seed coats (Tuteja et al., 2009 and Vodkin et al., 2012). However, it was not known if the same phenomenon applied to the yellow and pigmented regions within the same seed coats that have …
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Roles of piwi-interacting RNAs and small interfering RNAs in regulating gene expression in Caenorhabditis elegans
… RNAs (piRNAs), and small interfering RNAs (siRNAs). miRNAs are required throughout development, whereas piRNAs and siRNAs are best known for their roles in germline development and genome defense (see Chapter 1 for an overview of piRNA and siRNA pathways). To identify the roles of piRNAs and …
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The synthesis and in vivo evaluation of chemically modified siRNAs that contain red-shifted internal azobenzene derivative spacers for selective and tunable photocontrol of activity without UV light
Short interfering RNAs (siRNAs) are biopolymers that are used for post-transcriptional gene regulation and act as endogenous defenses against attack from viruses and dsRNA parasites. The siRNA field is making advances for use as pharmaceuticals and biomolecular research tools for the study of gene …
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The contribution of repeat associated small RNAs to genetic variation, hybrid vigor and inbreeding depression in maize
… in maize are the 24-nt small interfering RNAs (siRNAs) that silence abundantly repeated sequences such as TEs. In contrast to miRNAs and as observed previously in both Arabidopsis and rice, hybridization passes on parental differences in siRNA populations, and for those 24-nt siRNAs that do …
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The synthesis and in vitro evaluation of siRNAs modified at the backbone with a novel triazole-based internucleotide linkage and abasic alkyl-chain linkages of varying length
… use of double-stranded short interfering RNAs (siRNAs) to silence the expression of genes associated with disease at the translational level has gained much attention. Using a highly conserved endogenous pathway within cells, siRNA technology displays a high degree of target specificity and …
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The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
… is developing an effective delivery system for siRNAs. Recently, fatty acid conjugated siRNAs have shown promising potential as safe and effective method to deliver oligonucleotides to extrahepatic cells. A class of fatty acids that we are interested in are sphingolipids, they are a class of …
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Functional gene analysis in cultured vertebrate cells using siRNA mediated gene silencing
… RNAs (engl. kleine interferierende RNAs, siRNAs) in einem als RNA Interferenz (RNAi) bezeichneten Prozeß herunterreguliert werden. Die Anwendung von RNAi Technologie erlaubt die systematische Analyse von Genexpression und Genfunktionen. In dieser Dissertation characterisiere ich RNAi …
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Hemmung der humanen Telomerase Reverse Transkriptase-Expression mittels synthetischer Nukleinsäuren in Harnblasenkarzinomzellen
… (AS-ODN) und small interfering RNAs (siRNAs). In der vorliegenden Arbeit wurde die Expressionshemmung des potenziellen Targetgens hTERT (humane Telomerase Reverse Transkriptase) mit AS-ODN und siRNAs in BCa-Zellen untersucht. Die Tumorspezifität der hTERT-mRNA-Expression konnte …
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Exploring the specificity and mechanisms of siRNA-mediated gene silencing in mammalian cells
Complementary short interfering RNAs (siRNAs) are routinely used to knockdown gene expression. siRNAs bind to their target sequence and guide transcript cleavage and subsequent degradation. This type of silencing is associated with equivalent levels of RNA and protein knockdown. siRNA-mediated …
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Elucidating the role of effector caspases in immune development using lentiviral RNAi
… stranded RNAs, known as short interfering RNAs (siRNAs), through a mechanism that is well conserved across many species. While this technique has been used successfully in tissue culture experiments, our studies focused on extending the use of RNAi into immune cells. Our initial experiments …
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