Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"siRNA transfection"”.
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Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… nanoparticles (NP) that deliver silencing RNA (siRNA) exclusively to tumor cells for gene knockdown. Presently, nonviral delivery vectors, such as poly(B-amino ester) (PBAE) NP, are beneficial delivery systems because they are less immunogenic and easier to chemically modify than vectors …
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Ryanodine receptors in calcium signaling pathways
… signaling in the NG115-401L neuronal cell line. siRNA transfection methods were employed to knockdown RyR<sub>2</sub> expression levels in NG115-401L cells. We used reverse transcription and real-time PCR to evaluate RyR2 gene expression in transfected/untransfected cells. We also evaluated …
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Evaluating The Therapeutic Efficacy of Grb2 Inhibition In Ovarian Malignancies
… (cell viability assay, Western blot analysis, siRNA transfection, and reverse phase protein array) were carried out to elucidate the mechanism and potential predictors of tumor response to L-Grb2.</p> <p><strong>Results: </strong>Treatment with L-Grb2 decreased tumor growth and metastasis in …
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Einfluss der löslichen Guanylylzyklase auf die Expression der cGMP-Exporter MRP4 und 5 in humanen Melanomzellen unter Hypergravitationsbedingungen
… the mRNA-expression of the sGCb1 subunit through siRNA-transfection. The data show an increased expression of the investigated genes (MRP4/5, e/iNOS) after the cells were exposed to hypergravity (5g for 24 h), whereas no alteration could be found after silencing the sGCb1 subunit with siRNA. …
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Materials and biological approach to gene delivery in human embryonic stem cells
… a high degree of polymerization have a greater transfection efficiency and lower toxicity in IMR-90 cells than the commercial reagent Lipofectamine 2000. The usefulness of PEG-b-PVBLG-8 was further demonstrated via the successful transfection of hESCs without a measured loss in cell pluripotency …
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Polo-Like Kinase 1 and Smooth Muscle Cells in Coronary Artery Bypass Conduits and Coronary Arteries Following Angioplasty
… CDK1 phosphorylation. Silencing the PLK1 gene by siRNA transfection in SV- and IMA-SMCs attenuated the expression of p-Histone H3. Immunofluorescence staining demonstrated an increase in the number of cells showing immunopositivity to PLK1, pPLK1, p-Histone, IFN-γ and pSTAT-3 in the neointima in …
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Essentiality of Mitochondrial Genome Integrity in Cell Survival
… in HeLa and HCT116 p53 +/+ cells by siRNA transfection caused significant accumulation of SSBs/repair intermediates in the mitochondrial genome, depolarization of mitochondrial membrane potential, increase in ROS generation, and cell death. Increase in Annexin V positive cell …
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T-type calcium channels and human mesangial cell proliferation
… mechanism needing further investigation. MC transfection with siRNA targeting the α1H isoform resulted in significant knockdown of T-CaCN α1H mRNA and a reduction in the growth velocity of cultured MC of approximately 50% compared to control siRNA transfection, with an associated 37% …
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Investigations into the roles of Histamine Receptor H1 and extracellular vesicles in ovarian cancer cell behaviour
… OC cell lines (SKOV3, OVCAR3 and OVCAR5), via siRNA transfection, chemical activation (via histamine) or inhibition (via chlorpheniramine) and its effect on cellular behaviour was evaluated through different in vitro assays. Results showed that HRH1: 1) does not modulate gene expression of …
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Isoxazole Linked siRNA Cholesterol Conjugates by Catalyst Free Nitrile Oxide/Alkyne Cycloadditions
… the synthesIs of a range of chemically modified siRNAs by catalyst free Click Cycloaddition Chemistry. The first chapter overviews the structure and biological function of RNA, before turning to a discussion on the synthetic value of dipolar cycloaddition chemistry of nitrones and nitrile oxides …
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Materials and biological approach to gene delivery in human embryonic stem cells
… a high degree of polymerization have a greater transfection efficiency and lower toxicity in IMR-90 cells than the commercial reagent Lipofectamine 2000. The usefulness of PEG-b-PVBLG-8 was further demonstrated via the successful transfection of hESCs without a measured loss in cell pluripotency …
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Molecular Mechanism of Tetraspanin CD9 Mediated Cell Motility
… in CHO cells by dominant/negative PI-3K cDNA transfection abolished CD9 mediated pro-migratory effects. Consistent with these observations, CD9 expression in CHO cells and in the rat aortic smooth muscle (RASM) cells induced enhanced phosphorylation of PI-3K substrate, Akt. In CHO cells, CD9 …
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Signaling crosstalk in cancers
… of KRAS/MEK pathway activity by a KRAS specific siRNA or MEK inhibitors inhibits GLI transcriptional activity and GLI1 expression, and promotes GLI1 protein degradation in pancreatic cancer cell lines with activating KRAS mutation. Furthermore, Suppressing Gli activity leads to a selective …
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The Role of Connexin43 and Gap Junctional Intercellular Communication in the Apoptotic Process of Auditory Cells
… of gap junctional activity with either Cx43 siRNA transfection or a pharmacological drug, 18α-glycyrrhetinic acid in HEI-OC1 auditory cells caused a marked increase in cell viability with a decrease of apoptosis based on cleaved Poly (ADP-ribose) polymerase (PARP) and cleaved caspase 3 …
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The role of Ryanodine receptors in neuronal calcium signaling
… in NG115-401L cells. After testing different siRNA primers including their combination with different transfection reagent, the result shows a significant silencing effect to the RyR1 mRNA expression levels. In the second part of this study, we used a group of pharmacological agents with …
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Mechanism of Action of Nicotinamide Phosphoribosyltransferase Mediated Signaling in Oxidative Stress
… which measures cellular viability. Testing of siRNA transfection mediated knockdown of endogenous Nampt was performed in the retinal pigment epithelial cell line ARPE-19 and primary isolated optic nerve head astrocytes as models for cells supporting the viability and function of retinal …
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The Role of Acidic Fibroblast Growth Factor and Fibroblast Growth Factor Receptor 4 In High Grade Serous Carcinoma
… expression was then transiently silenced via siRNA transfection and the effects on response to FGF1, cellular proliferation, and migration were quantified. To identify relevant cellular pathways involved, responsive cell lines were transduced with different transcription response elements …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… substrate, aptly named short-interfering RNA (siRNA), which binds to and triggers the degradation of a targeted complementary mRNA strand within the catalytic site of the RNA-Induced Silencing Complex (RISC). In this manner, malignant mRNA expression is silenced, thereby inhibiting the …