Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"siRNA screening"”.
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RNAi Nanotechnology: A Platform for siRNA Screening and Cancer Gene Therapy
… micro RNA (miRNA), short-interfering RNA (siRNA), small nuclear RNA (snRNA) and many others. The noncoding RNAs, such as siRNA, do not express proteins but have been utilized in a wide range of applications, including RNA interference (RNAi) and the regulation of mRNA expression. These …
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MAPK9: Ein neuer Regulator des Triglyzerid-Stoffwechsels, entdeckt durch siRNA Screening
… new players involved in lipid regulation, a screening procedure was developed combining RNA interference in Hela cells and thin layer chromatography. Thereby, it is possible to monitor modifications of lipid composition resulting from siRNA knock-down. Furthermore, the potential of mass …
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Identification of regulators in autophagosome formation using image-based siRNA screening
… in this field. Here we conducted a genome-wide siRNA screen using a high-throughput imaging system to identify undiscovered regulators in autophagosome formation. In this study, HEK293 cells stably expressing GFP-DFCP1 (GFP-tagged zinc finger FYVE-type containing 1) were used and amino acid …
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Host Modulators of the Death Response to Influenza A Infection
… Here I employed arrayed whole-genome siRNA screening strategies to identify cell-autonomous molecular components that are subverted to support H1N1 influenza A virus infection of human mucosal epithelial cells. Integration across relevant public data sets exposed druggable gene …
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Genomic analysis of hepatic insulin resistance
… differentially expressed genes and combinatorial siRNA screening to investigate the effects of hepatic gene transcription during periods of high and low glucose production. This strategy provides a new approach to studying the molecular mechanisms of disease pathogenesis. Our investigations …
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MODULATING TFEB ACTIVITY IN THE BIRT-HOGG-DUBE' SYNDROME BY TARGETING THE VACUOLAR ATPASE
… of the BHD syndrome. We performed a high content siRNA screening to identify correctors of TFEB activation in FLCN-KO cells. Interestingly, top hits were genes encoding subunits of the vacuolar ATPase (V-ATPase). The V-ATPase is a proton-pump protein complex responsible for maintaining the low pH …
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Developing Novel Therapies Targeting the Tumor Microenvironment of Aggressive Breast Cancer
… therapy, we conducted a synthetic lethal kinome siRNA screening in vitro to identify novel synergy targets. We found TANK-binding kinase 1 (TBK1) to be the top therapeutic candidate due to its critical role in immune responses. Strikingly, combined suppression of AXL and TBK1 significantly …
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Molecular Mechanisms of Itch Sensations
… present in the spinal GRPR neurons. Differential screening and pharmacological or small interfering RNA: siRNA) approach identified four histamine-independent: Atp2a1, Pld3, Pacsin3 and Itpr3), three histamine-dependent: S100a8, CALR and DSP) and one both-dependent: TRPV4) itch genes. Atp2a1 and …