Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"siRNA screen"”.
-
Investigation into ubiquitin signalling in response to cisplatin
… describes the use of a robust high throughput siRNA screen to identify such novel ubiquitin-associated components of the response to cisplatin. Positive candidates were identified based on their ability to cause sensitivity to cisplatin and included both known and novel mediators of the ICL …
-
A pathways-based approach identifying determinants of drug resistance in oesophageal adenocarcinoma
… regulated between the two groups. A focused siRNA screen of 80 target genes identified from the GSEA results was then devised and completed, highlighting the role of Src in chemotherapy non-responders. A compound screen of FDA-approved drugs was carried out in the OE33 and FLO-1 cell lines in …
-
Calpain 9 Functions in TNF Receptor Mediated Apoptosis
… I performed a genome-wide high-throughput siRNA screen and identified Calpain 9, CAPN9, as a novel component of TNF-alpha induced apoptosis. CAPN9 knockdown does not affect TNF-a secretion, yet is essential for downstream activation. Two splice variants are reported for CAPN9. The smaller …
-
Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen
… I conducted the first genome-wide, imaging-based siRNA screen to identify regulators of LAD positioning using HiDRO (high-throughput DNA or RNA labeling with optimized Oligopaints). Completion of the screen led to the identification of 101 genes required for proper perinuclear localization. …
-
Identification of Oncogenic KRAS-Associated Vulnerabilities in Non-Small Cell Lung Cancer
… with RNAi-based primary synthetic-lethal screens; a prevalent miRNA-like behavior of siRNA and cell line-dependent phenotypic diversity within intra-lineage KRAS-driven cancer. In consideration of these, we performed a whole-genome synthetic-lethal siRNA screen, powered by 106 NSCLC lines …
-
Therapeutic Targeting of BMP and TGF-β Signalling Pathways for the Resolution of Pulmonary Arterial Hypertension
… through the BMPRII-ALK1-BclxL axis. Finally, the siRNA screen targeting approximately 1000 genes has identified novel proteins including PPP1CA, IGF-1R, MPP1, MCM5 and SRC each capable of modulating the BMPRII signalling. Taken together, this study for the very first time has identified novel …
-
Characterisation of Androgen Receptor Signalling and Metabolism in Prostate Cancer
… Previously, using a small interfering RNA (siRNA) screen, haem synthesis was identified as a potential therapeutic target for prostate cancer and the pathway was therefore further investigated. The results demonstrated that inhibiting haem synthesis, using succinylacetone, significantly …
-
Genetic and Chemical Genetic Approaches to Shiga Toxin Inhibition
… One approach relied on a high-throughput screen of a small compound library to identify potential inhibitors of Shiga toxin intracellular transport, as Shiga toxin is known to undergo a stepwise progression through host cells following endocytosis. This screen identified three compounds …
-
Real-Time Bioluminescence Imaging Of Salmonella-Neoplastic Cell Interactions
… interactions, I utilized two high-throughput screens. In the first, I designed a bioluminescent transposon-reporter trap to identify specific Salmonella genes activated in the context of cancer cell co-culture conditions. Through this work, I identified five Salmonella genes reproducibly …
-
Molecular analysis of insulin action using high throughput genetic screens
… and optimized for use in high throughput screening for regulators of insulin stimulated GLUT4 translocation. Insulin stimulation caused GLUT4 translocation to the plasma membrane, and the activation of the PI3K/Akt signaling pathway in these cells. Using this cell line, I performed an …
-
Novel Techniques to Target Androgen Signalling in Prostate Cancer
… enzymes to promote PCa growth. Hence an siRNA screen targeting 237 different genes involved in metabolism and cell traffic was performed in order to identify novel metabolic targets to inhibit PCa growth. LNCaP cells were transfected with the siRNAs and proliferation measured via crystal …
-
An investigation into the role of LUBAC and linear ubiquitin chains in antibody dependent intracellular neutralisation
… is recruited to the ADIN pathway. I performed an siRNA screen targeting known VCP adaptors using a TRIM21- dependent adenovirus neutralisation model. I found that HOIP, the only E3 ubiquitin ligase capable of generating linear ubiquitin chains, is required for efficient neutralisation of …
-
Identification of regulators in autophagosome formation using image-based siRNA screening
… in this field. Here we conducted a genome-wide siRNA screen using a high-throughput imaging system to identify undiscovered regulators in autophagosome formation. In this study, HEK293 cells stably expressing GFP-DFCP1 (GFP-tagged zinc finger FYVE-type containing 1) were used and amino acid …
-
Autophagy in Antiviral Immunity
… infection. Lastly, we performed a genome-wide siRNA screen to identify novel host factors required for autophagic targeting of viral proteins. We identified previously unappreciated cellular networks and genes that were involved in targeting viral proteins for autophagy. One of these factors, …
-
Regulation of Human Telomerase Alternative Splicing
… 528 RNA-binding proteins/splicing factors siRNA screen. Our initial validation focused on 45 factors with enzymatic activity and resulted in CDC-Like Kinase 1 (CLK-1) as a potential hTERT splicing modulator. Knock-down of CLK-1 altered hTERT splicing, resulting in reduction in telomerase …
-
Association of Protein Phosphatase 2A with S6 Kinase Is Regulated In an MTOR-Dependent Manner
… upon stimulation of the pathway by insulin. An siRNA screen targeting PP2A regulatory subunits was used to identify specific subunits that were mediating this association. Depletion of the B56gamma and B56delta regulatory subunits reduced co-immunoprecipitation of PP2A with S6K1. However, …
-
The Role of Adaptor Protein Grb2 in Ecotropic Retrovirus Entry and Receptor Trafficking
… amino acid transporter 1 (mCAT1). Using a siRNA screen to detect host cell components important for virus infection, we identified growth factor receptor-bound protein 2 (Grb2), as a key protein for eMLV infection. Grb2 is an adaptor that has been shown to couple cell surface receptors such …
-
MiR-10a Regulation of Drug Response and Cancer Stem Cell Populations in Non-Small Cell Lung Carcinomas
… the direct target of miR-10a we used a targeted siRNA screen containing genes implicated in stem cell maintenance to reveal that the WNT and Notch pathway are important for cell survival. Both pathways are down-regulated when cells are treated with a miR-10a mimic. Bioinformatic analysis …
-
Vinexin regulates autophagy through YAP/TAZ: implications for health and disease
… dissertation builds on published data from an siRNA screen for autophagy regulations under basal conditions, which indicates vinexin knockdown upregulates autophagy. I replicate this finding in multiple cell lines, before characterising the impact of siSORBS3 treatment on autophagy; …
-
Novel insights into the molecular mechanisms of endocrine resistance in ERα positive breast cancer
… resistance was confirmed using an independent siRNA screen. In addition, the direct contribution of ETV6 to breast cancer progression was proved by the promoting effects of ETV6 overexpression on colony formation ability of endocrine sensitive cell lines. Furthermore, chromatin …
Page 1 of 2