Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"siRNA delivery"”.
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Designing nanoparticles for highly efficient endothelial siRNA delivery
… it to specific cells in vivo. In vivo RNA delivery is challenging; vehicles must avoid phagocytosis in the bloodstream, reach the target tissue, and get into, and out of, an endosome, all without setting off an unwanted immune response. Despite these challenges, nanoparticles have delivered …
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Novel cell-penetrating peptide-based vectors for siRNA delivery
Harnessing the RNAi pathway with synthetic siRNA as a potent and selective mode of post-transcriptional gene silencing and has therapeutic potential in personalised medicine; however, the large size and negative charge of siRNA creates a hurdle for intracellular delivery that has thus far limited …
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Self-Assembled Gold Nanoplexes for Cancer-Targeted siRNA Delivery
… Moreover, the Au nanoplexes can protect siRNA from RNase degradation and are stable in cell culture medium.</p> <p>siGLuc-ppp, ssRNA<sub>80</sub>, and siF17 were delivered by the assembled Au nanoplexes, and the results were analyzed by GLuc assay, TB assay, and Luciferase assay. In terms …
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Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… standard cancer therapeutics is the use of drug delivery systems such as polymeric nanoparticles (NP) that deliver silencing RNA (siRNA) exclusively to tumor cells for gene knockdown. Presently, nonviral delivery vectors, such as poly(B-amino ester) (PBAE) NP, are beneficial delivery systems …
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Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
<p>The delivery of nucleic acid therapeutics across various cell membranes has always been one of the major challenges. Cell-penetrating peptides (CPPs) containing arginine (R), tryptophan (W), and histidine (H) have been promising for siRNA delivery. Therefore, to enhance siRNA delivery and …
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Elucidation of Design Criteria for siRNA Delivery in Mammalian Cells Using Polyethylenimine
… the issue of how PEI concentration affects siRNA delivery efficiency. It also examines how PEI derivatives affect the three primary in vitro barriers: (1) cellular uptake, (2) endosomal escape, and (3) unpackaging of PEI/siRNA polyplexes. Chapter 2 will demonstrate that high knockdown …
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Endocytosis mechanism of poly- and lipoplexes in siRNA delivery in HeLa cells
… and it is initiated by small interfering RNA (siRNA) molecule. siRNA is a 19 nucleotides-long double stranded RNA-molecule. In order to begin gene silencing, siRNA needs to be delivered to the cytosol. The first of the barriers that stand on the path of the siRNA delivery is endocytosis. …
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Synthesis and Evaluation of Carbohydrate Based Cationic Polymers for siRNA Delivery and Tracking
… in medical applications. However, inadequate delivery of RNA molecules to intracellular target sites has become the bottleneck in both laboratory use and clinical therapy in the future. In general, the small interfering RNA molecules as well as other RNA molecules have poor pharmacokinetic …
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Engineering Lipid Nanoparticles for sIRNA Delivery to Silence Identified Targest in Cancer Cells
… and optimization of an LNP platform designed for siRNA delivery, addressing critical challenges such as cellular uptake, cytotoxicity, and gene silencing efficiency. While the scope of this work is limited to LNPs, it lays the foundation for the future development of a lipid-polymer nanoparticle …
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Hybrid Cyclic/Linear Peptides in a Multi-Component Lipid Structure as a siRNA Delivery System
<p>Delivering siRNA is challenging due to many obstacles, such as extremely short in vivo half-life, rapid elimination via glomerular filtration, and inability to cross cell membranes due to the hydrophilic and negatively-charged nature. Thus, a delivery system is needed to deliver the siRNA into …
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Synthesis, Characterization, and Evaluation of Amphiphilic Cell-Penetrating Peptides as siRNA Delivery Tools for Cancer Therapy
… possess a great therapeutic potential. Three siRNA-based products have been recently approved that have developed a renewed interest in the scientific community to explore this alternative therapeutic area rigorously. However, several challenges are limiting their use as a widespread …
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Evaluation of therapeutic effect of IKK epsilon SiRNA on breast cancer cells and development of a peptide-based SiRNA delivery system
The objective of this dissertation is to develop siRNA-based cancer therapeutics. In chapters 1 and 2, we introduced the mechanism, challenge and promise of RNAi-based therapy, as well as various siRNA delivery systems. In Chapter 3, we examined the therapeutic potential of IKKε siRNA on human …
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Tumor-penetrating delivery of small interfering RNA therapeutics
… RNAi into the clinic has been thwarted by the "delivery" challenge, as small interfering RNA (siRNA) therapeutics must overcome clearance mechanisms and penetrate into tumor tissues to access cancer cells. This thesis sought to develop nanotechnology-based platforms to rapidly discover and …
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Non-viral drug delivery systems for immune modulation
… particles have diverse applications in drug delivery. The main objective of this thesis was to apply these delivery systems to modulating the immune system. We optimized particle formulations for the delivery of three novel immune modulating compounds, small inhibiting RNA, immunostimulatory …
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Understanding barriers to efficient nucleic acid delivery with bioresponsive block copolymers
The delivery of nucleic acids has the potential to revolutionize medicine by allowing previously untreatable diseases to be clinically addressed. Viral delivery systems have been held back by immunogenicity and toxicity concerns, but synthetic vectors have lagged in transfection efficiency. This …
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Conjugation of Zwitterionic and Cationic Peptides to Lipid and Polymer for Gene Delivery
… and the backbone to achieve desired results for siRNA delivery.
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Mouse Models of Inherited Skin Diseases
… mutant KRT9 allele with short interfering RNAs (siRNAs) was explored as a potential therapeutic approach. This led to the development and in vitro validation of potent mutation- and gene-specific siRNA inhibitors. <br/>Despite its importance in EPPK, little is known about the physical requirement …
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Engineering periodic short hairpin RNA delivery systems for enhanced therapeutic efficacy
… by barriers in delivering short interfering RNA (siRNA). Numerous delivery vehicles have been developed to protect siRNA from degradation, promote target cell uptake, and facilitate endosomal escape into the cytoplasm, where RNAi occurs. However, in vivo instability, low silencing efficiency, …
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A comparative study of cationic formulations for the delivery of siRNA and DNA
… of RNAi, delivering the small interfering RNA (siRNA) to the cells is one of the main hurdles. Previously there have been reports showing effective plasmid DNA delivery to cells and such systems could be used to deliver siRNA to cells due to the similarity of the delivery criteria between …
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Magnetically-driven Therapeutic Agents Delivery System using Iron Oxide Nanocages and Enhancement of Exosome Secretion, a Potential Biological Drug Delivery Carrier
… on using 20 nm iron oxide nanoparticles as siRNA delivery carriers under the alternating magnetic field and the development of a method to amplify the secretion of 150 nm exosomes from the cells, which could potentially use as a biological drug carrier.</p> <p>Chapter 2 discusses a …
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