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Showing 1 to 20 of 62 for “"shotgun cloning"”.

  1. Molecular cloning, structural characterisation and functional study of bioactive peptides from amphibian skin secretion

    Chen, YuqingQueen's University Belfast2021

    … by use of electrical stimulation. Afterwards, ‘shotgun’ cloning was applied using 3’RACE PCR to obtain full length nucleotide sequence that encoded the open reading frames of peptide biosynthetic precursors. Three putative peptides were identified by cloning (Kunitzin-AH, QUB-1617, QUB-1629), …

    qu-belfast Repository record

  2. A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i>

    Lu, KeleiQueen's University Belfast2017

    … torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation …

    qu-belfast Repository record

  3. Bioactive evaluations of novel peptide QUB-2552 from the skin secretion of the Chinese Large Odorous frog, Rana (Odorrana) livida

    Zhang, YujingQueen's University Belfast2018

    … frog, Rana (Odorrana) livida, by applying ‘Shotgun’ cloning method. The novel peptide was synthesised by using Solid Phase Peptide Synthesis strategy. After the synthetic peptide was identified and purified by Matrix-assisted, laser desorption, ionisation, time-offlight (MALDI-TOF) and …

    qu-belfast Repository record

  4. Identification, isolation and bioactivity assessment of a novel peptide from the skin of Heilongjiang Brown Frog (Rana amurensis)

    Gan, XiaoyangQueen's University Belfast2018

    … peptide named QUB-2207 was identified through “shotgun” cloning of biosynthetic precursor-encoding cDNA from the skin secretion of the Heilongjiang Brown Frog, Rana amurensis. The peptide was chemically-synthesised by solid phase peptide synthesis. Antimicrobial assays and haemolytic assays were …

    qu-belfast Repository record

  5. The discovery of a novel bioactive peptide, QUB-1036, from the defensive skin secretion of Pelophylax kl. esculentus

    Cui, QingyuQueen's University Belfast2017

    … frog, Pelophylax kl. esculentus, by both a ‘shotgun’ cloning genomic study and a peptidomic study. After chemical synthesis, QUB-1036 was subjected to bioactivity tests. As a result, QUB-1036 was observed to have no inhibition against microbial growth and was completely devoid of haemolytic …

    qu-belfast Repository record

  6. A bioactive peptide, QUB-1813, from the defensive skin secretion of the Chinese Large Odorous Frog, Rana (Odorrana) livida

    Fang, QiaoyuQueen's University Belfast2017

    … Odorous frog, Odorrana livida. In this study, “shotgun” cloning was employed to identify a peptide named QUB-1813 from the skin secretion and online BLAST sequence analysis was also performed. The structure of the peptide was initially deduced from cloned precursor-encoding cDNA and confirmed by …

    qu-belfast Repository record

  7. QUB-1383, a novel bioactive peptide from the defensive skin secretion of the edible frog (Pelophylax kl. esculentus)

    Sang, MengruQueen's University Belfast2017

    … the edible frog (Pelophylax kl. esculentus) via “shotgun” cloning of the precursor-encoding cDNA. Afterwards, the predicted mature peptide was chemically synthesised by solid-phase peptide synthesis and its structure confirmed via MALDI-TOF mass spectrometry. Then the crude synthesised peptide was …

    qu-belfast Repository record

  8. The discovery and evaluation of a novel bioactive peptide, QUB-3112, from the defensive skin secretion of Hylarana guentheri

    Lu, HuiminQueen's University Belfast2017

    … skin secretion of Hylarana guentheri by using a ‘shotgun’ cloning method. To research the biological functions of QUB-3112, it was synthesised by using solid phase peptide synthesis (SPPS) according to the translated putative mature peptide sequence. The synthetic replicate was used to perform …

    qu-belfast Repository record

  9. Analysis of a novel antimicrobial peptide QUB-1746 from the skin secretion of the frog, Kassina senegalensis

    Wu, HuiQueen's University Belfast2019

    … skin secretion derived cDNA library through ‘shotgun’ cloning. The mature peptide was synthesized by solid phase peptide synthesis (SPPS). RP-HPLC and MALDI-TOF MS were used to purify and analyze the mature peptide QUB-1746. Its minimal inhibitory concentration (MIC) was detected by using …

    qu-belfast Repository record

  10. QUB-2577: A Bioactive Peptide from Pickerel Frog (Rana palustris) Skin Secretion

    Xu, WeiyueQueen's University Belfast2018

    … thesis, the peptide QUB-2577 was identified by "shotgun" cloning of precursor-encoding cDNA derived from the skin secretion of Rana palustris. By applying solid-phase synthesis (SPPS), the peptide was synthesised. After purification, its antimicrobial, anticancer and other biological activities …

    qu-belfast Repository record

  11. QUB-1951: a bioactive peptide from the skin secretion of the Chinese piebald Odorous frog, Odorrana schmackeri

    Hu, JiayiQueen's University Belfast2018

    … precursor encoded cDNA was identified by using “shotgun” cloning, with the amino acid sequence of putative mature peptide, AVNIPFKVHFRCKAAFC. In order to obtain sufficient peptides to carry out the bioactive assays, solid phase peptide synthesis (SPPS) was used to synthesize. The molecular weight …

    qu-belfast Repository record

  12. The discovery of a novel peptide, QUB-2023, from Rana amurensis

    Han, KunQueen's University Belfast2017

    … of Rana amurensis. This was achieved by “shotgun” cloning. Solid-phase peptide synthesis (SPPS) and Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) were applied to obtain a sufficient quantity of pure peptide replicate. The primary structure of the peptide was confirmed by …

    qu-belfast Repository record

  13. NGC-HLB, a novel antimicrobial peptide from the skin secretion of Hylarana latouchii

    Bao, KaifanQueen's University Belfast2017

    … between the masses of the peptide obtained from “shotgun” cloning and MALDI-TOF MS. NGC-HLB possessed broad-spectrum antimicrobial activity against Gram-positive Staphylococcus aureus (NCTC 10788), Gram-negative Escherichia coli (NCTC 10418) and the yeast Candida albicans (NCPF 1467) with minimal …

    qu-belfast Repository record

  14. A novel bioactive peptide from the defensive skin secretion of Phyllomedusa sauvagii

    Shi, YunfeiQueen's University Belfast2017

    … secretion of Phyllomedusa sauvagii. Firstly, “shotgun” cloning was used to obtain the nucleotide and translated open-reading frame (ORF) amino acid sequence of this precursor-encoding cDNA. BLAST analysis indicated that the amino acid sequence of the novel peptide QUB-2909 …

    qu-belfast Repository record

  15. Evaluation of bioactivity of QUB1977 from the skin secretion of the frog, Phyllomedusa camba

    Wu, XianhuiQueen's University Belfast2017

    … secretion of the frog, Phyllomedusa camba. A “shotgun” cloning technique was used to select the biosynthetic precursor sequence and a cDNA library was constructed for amplification of the target sequence. The peptide was isolated and identified using HPLC, MALDI-TOF mass spectrometry.<br/>The …

    qu-belfast Repository record

  16. Isolation, characterisation and molecular cloning of a bioactive peptide, QUB 1675, from the defensive skin secretion of Odorrana livida

    Yang, WeiQueen's University Belfast2017

    … precursor of QUB 1675 was identified by using “shotgun” cloning of a cDNA library generated from the polyadenylated mRNA present in the skin secretion of Odorrana livida. To evaluate the bioactivity of this peptide, QUB 1675 was synthesised by solid phase peptide synthesis (SPPS). The synthetic …

    qu-belfast Repository record

  17. Identification and bioactivity assessment of a protease inhibitor QUB-1833 from the skin secretion of Lithobates catesbeiana

    Liu, WenqingQueen's University Belfast2020

    … catesbeiana skin secretions.<br/><br/>Using 'shotgun' cloning and DNA sequencing, the peptide sequences were obtained from secretions. Then, the peptide QUB-1833, whose amino acid sequence is SLRGCWTKSYPPQPCL, was selected as the research object. After synthesized by SPPS and purified by …

    qu-belfast Repository record

  18. Identification and activity assessment of a bioactive peptide QUB-2642 from the skin secretion of The American Bullfrog (Rana catesbeiana)

    Liu, XinyueQueen's University Belfast2020

    … of The American Bullfrog (Rana catesbeiana) by ‘shotgun’ cloning. Using synthetic peptides, subsequent antimicrobial and haemolysis assays demonstrated that QUB-2642 had strong inhibitory activity against bacterial strains Staphylococcus aureus (MIC = 8 µM) and Escherichia coli (MIC = 8 µM) as …

    qu-belfast Repository record

  19. Discovery and bioactivity assessment of a peptide QUB-1331 from the skin secretion of the Senegal running frog (Kassina senegalensis)

    Liu, YimanQueen's University Belfast2020

    … senegalensis, a Senegal running frog via “shotgun cloning” strategy. After the peptide QUB-1331 precursor-encoding cDNA was obtained, Solid Phase Peptide Synthesis (SPPS) was applied to synthesise the peptide QUB-1331. Subsequently, RPHPLC was used to separate QUB-1331 from the impure …

    qu-belfast Repository record

  20. Characterized analysis of peptide QUB-1433 from the skin secretion of the dark-spotted frog (Pelophylax nigromaculatus)

    Wang, WenjieQueen's University Belfast2019

    … skin secretion of Pelophylax nigromaculatus via “shotgun” cloning. According to the amino acid residues of QUB-1433, the pure peptide was synthesised and purified via solid-phase peptide synthesis and RP-HPLC. Subsequently, antimicrobial, anticancer and haemolysis assays were performed on QUB-1433 …

    qu-belfast Repository record

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