NDLTD logo NDLTD Union Catalog and Global ETD Search

Global ETD Search

Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

Results

Showing 1 to 20 of 22 for “"short-hairpin RNA (shRNA)"”.

  1. Identification of Small Molecule Inhibitors Targeting Truncated Adenomatous Polyposis Coli (APC) as a Novel Therapeutic Strategy in Colorectal Cancer

    Zhang, Lu2014

    … independent of canonical Wnt pathway activity. Short hairpin RNA (shRNA) mediated knockdown of truncated APC confers resistance to TASIN-1. Additionally, TASIN-1 can interact with in vitro translated APC fragments, implicating truncated APC in the mechanism of action of TASIN-1. TASIN-1 inhibits …

    tdl Repository record

  2. Identification of Small Molecule Inhibitors Targeting Truncated Adenomatous Polyposis Coli (APC) as a Novel Therapeutic Strategy in Colorectal Cancer

    Zhang, Lu2016

    … independent of canonical Wnt pathway activity. Short hairpin RNA (shRNA) mediated knockdown of truncated APC confers resistance to TASIN-1. Additionally, TASIN-1 can interact with in vitro translated APC fragments, implicating truncated APC in the mechanism of action of TASIN-1. TASIN-1 inhibits …

    utswmed Repository record

  3. Silencing mutant Huntingtin by RNA interference for the treatment of Huntington Disease

    Wagner, Laura A.University of British Columbia2008

    … testing to develop potential treatments for HD. RNA interference (RNAi) therapy is a targeted gene silencing approach whereby synthetic RNA constructs are shuttled into the cell by viral vectors and used by the cell’s endogenous RNAi machinery to silence a gene of interest. RNAi therapy holds …

    ubc Repository record

  4. Phosphatidylinositol 4-phosphate 5-kinase Involved in Neuroglial Toll-like Receptor Signaling

    NGUYEN, THI NGOC TU2012

    … of BV2 microglia were developed using lentiviral short hairpin RNA (ShRNA) expression system. PIP5K-alpha ShRNA significantly reduced PIP5K-alpha protein and mRNA levels. PIP5K-alpha knockdown significantly suppressed LPS-induced production of inflammatory mediators, such as interleukin IL-6, …

    ajou Repository record

  5. Characterizing the Role of CDK2AP1 in Primary Human Fibroblasts and Human Embryonic Stem Cells

    Alsayegh, Khaled2018

    … In the first part of this study, using a short-hairpin RNA (shRNA) approach, we investigated the effect of CDK2AP1 downregulation in HDFs. Outcomes indicated: (a) reduced proliferation, (b) premature senescence, (c) cell cycle alterations, (d) DNA damage, and (e) an increase in p53, p21, …

    vcu Repository record

  6. Mitochondrial Unfolded Protein Response Regulator Atf5 In Mitochondrial Targeted Therapies In Aml

    Zhao, Ran, <p><strong>0000-0003-3390-5343</strong></p>2021

    … demonstrated that stable knockdown of ATF5 by short-hairpin RNA (shRNA) sensitized AML cells to single treatments of ONC201 and gamitrinib. In contrast, ATF5 knockdown did not further enhance AML cell killing when these agents were combined with the BCL2 inhibitor venetoclax, suggesting that …

    uthsc Repository record

  7. Biological Confinement of Zebrafish Using RNAi

    Boratynska, SusanUniversité d'Ottawa / University of Ottawa2015

    … to the natural populations does not occur. RNA interference (RNAi) is an endogenous mechanism used to regulate gene expression by destroying targeted mRNA molecules. Manipulation of this biological process has been successfully utilized to knockdown specific genes through the introduction of …

    ottawa-retro Repository record

  8. IN VIVO AND IN VITRO EVALUATION OF THE COMBINATION OF RNA INTERFERING AND GENE THERAPY FOR TREATING MITOFUSIN2-RELATED DISEASES

    ABATI, ELENAUniversità degli Studi di Milano2024

    … defect of CMT2A, based on the combination of RNA- interference (RNAi) and gene therapy. Methods: We devised a strategy whereby both the mutant and wild-type MFN2 mRNA are inhibited by a short hairpin RNA (shRNA), while the wild-type protein is restored by overexpressing a cDNA encoding …

    milano Repository record

  9. Bst2 Isoforms: Distribution and Potential Use In Cancer Therapeutics

    Muhsin, Ahmed, <p>0000-0002-5834-9921</p>2021

    … a loss-of-function approach with BST2-targeting short hairpin RNA (shRNA) in the T47D breast cancer cell line. Silencing BST2 was associated with a statistically significant reduction in the viability of T47D (p value < 0.05) but had no impact its proliferation (p value = 0.6). Lastly, the …

    uthsc Repository record

  10. DNA Damage and Cell Death Mediated by Thymidylate Synthase Inhibitors: Role of UNG and SMUG1 DNA Glycosylase

    Nagaria, Pratik K

    … increasing the uracil (U) content. Alternatively, 5-FU metabolites can also cause DNA damage by direct incorporation into DNA or RNA. Incorporation of U and 5-FU activates the DNA glycosylase-initiated base excision repair (BER) in cells to remove the damaged base and replace it with a …

    south-carolina Repository record

  11. Differential Angiogenic Capability and Hypoxia Responses in Glioma Stem Cells

    Li, Zhizhong2009

    … response. Targeting HIF1a or HIF2a in GSCs using short hairpin RNA (shRNA) inhibited neurosphere formation efficiency, indicating a requirement for HIFs in cancer stem cell self-renewal. HIF1a and HIF2a were also necessary for VEGF expression in GSCs, but HIF2a was not required in matched non-stem …

    duke Repository record

  12. Investigation of the role of neuroserpin in neurite outgrowth and learning and memory

    Tsang, VickyResearchSpace@Auckland2013

    … Neuroserpin knockdown was achieved by the use of short hairpin RNA (shRNA) which led to changes in the neurite extension and cell morphology of the PC12 cells. When quantitative measures of cell and neurite morphology were related to the level of neuroserpin knockdown achieved by different shRNA …

    auckland-ms Repository record

  13. Desarrollo de sistema lentiviral para la codificación de un short hairpin RNA que permite el silenciamiento dual de las oncoproteínas E6 y E7 de HPV16 en células de pulmón humano

    Parra Rivas, Bárbara AndreaUniversidad de Chile2013

    … cáncer. La producción de un intermediario de siRNA denominado short-hairpin RNA (shRNA), es un sistema eficiente de supresión de un transcrito blanco específico, que puede actuar durante varias generaciones celulares. Los vectores lentivirales son un sistema seguro y eficiente de …

    chile Repository record

  14. Molecular Mechanisms of Host Responses to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection

    Catanzaro, NicholasVirginia Tech2020

    … SGs are intracellular, cytoplasmic aggregates of RNA-binding proteins (RBPs) and mRNA. Formation of SGs is observed upon cellular stress and ultimately function to arrest cellular translation to promote cellular survival until the stress has been remedied. Indeed, several viruses have been shown …

    vt Repository record

  15. Viral and Host Factors Associated with HIV-1 Vertical Transmission and Pathogenesis

    Mehta, RoshniThe University of Arizona.2008

    … level of cellular factors (IL6 and STAT3) using short hairpin RNA (shRNA) technology to determine if these cellular factors were playing a role in an increased HIV-1 replication and gene expression. I found that upon downregulation of these factors, there was a decrease in HIV-1 LTR directed gene …

    arizona-thes Repository record

  16. The role of the extracellular matrix in neuronal development and perinatal brain injury

    Fowke, TaniaResearchSpace@Auckland2018

    … cortical neurons in vitro, (2) determine the short-term effect of HAS inhibition on neuronal morphology in vitro, and (3) determine the longer term effect of selective HAS knockdown on neuronal morphology in vitro. The final aim (4) was to assess the effects of cerebral hypoxia-ischemia (HI) …

    auckland-ms Repository record

  17. Investigating mechanisms and biological functions of the bHLH/PAS transcription factors SIM1 and ARNT2

    Gerassimou, Alexis Georgia2021

    … The development of a doxycycline (Dox) inducible short hairpin RNA (shRNA) system is an attractive approach to deplete gene expression without irreversibly damaging a gene and can allow normal embryonic development to occur prior to studying effects of reduced protein levels in adult tissues. In …

    adelaide Repository record

  18. Dual regulation of transcription factor Nrf2 by Keap1 and the beta-TrCP/GSK-3 in cancer

    Ebisine, KimimuepighaUniversity of Dundee2019

    … a role in the development of such cancer. Using short hairpin RNA (shRNA) and the CRISPR/Cas9 system to generate stable Nrf2 knockdown A549 and H460 cells, the second part of this thesis investigated biochemical and physiological changes that occur, when the Nrf2 is genetically downregulated, and …

    dundee Repository record

  19. Molecular Genetic Analysis of Spinocerebellar Ataxia Type 7 and a Further Study on the RNA Interference Analysis

    장, 석훈2011

    … therapy. More recently, gene silencing through RNA interference (RNAi) by double-stranded RNA (dsRNA) has emerged as a powerful method to inhibit expression of targeted gene. This new method provides a unique tool for sequence-specific gene suppression and may hold great promise for a potential …

    ajou Repository record

  20. The Role of Multi-Drug Resistance Associated Protein 4 and P-glycoprotein in Resistance of Neuroblastoma to Topotecan and Irinotecan

    Turner, Patricia Kellie2016

    … resistant to topotecan. Furthermore, mRNA expression of the adenosine triphosphate (ATP)-binding cassette (ABC) transporters P-glycoprotein (Pgp) and multidrug resistance associated protein 1 (MRP1), which efflux many drugs used in neuroblastoma therapy, has been implicated in poor …

    tenn-hsc Repository record

Page 1 of 2