Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"short interfering RNAs"”.
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Synthesis, characterization and biological investigation of self-delivering and modified short interfering RNAs (siRNAs)
… by targeting and degrading mRNA. Synthetic short interfering RNAs (siRNAs) can use this endogenous machinery and have emerged as a novel class of gene-silencing therapeutics. Unfortunately, the development of RNAi therapeutics has been hindered by several challenges associated with the …
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The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
… the pre-translational level through the use of short interfering ribonucleic acid (siRNA) has become a widely studied area since the discovery of the RNA interference pathway in 1998. The ability to silence genes at this point in the central dogma of cell biology offers the chance for excellent …
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Synthesis of internal amide bond short interfering RNAs (siRNAs) and investigation of their gene silencing properties
… of these molecularly-targeted therapeutics are short interfering RNAs (siRNAs). These siRNAs serve as the effectors of RNA interference, a naturally occurring and highly specific mechanism for regulating gene expression through sequence-specific degradation of messenger RNA. However, the native …
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Endogenous short interfering RNAs that silence chalcone synthase result in pigment pattern formation on soybean seed coats
… (CHS) genes leading to production of CHS siRNAs which in turn degrade CHS mRNAs resulting in yellow seed coats (Tuteja et al., 2009 and Vodkin et al., 2012). However, it was not known if the same phenomenon applied to the yellow and pigmented regions within the same seed coats that have …
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The synthesis and in vitro evaluation of chemically modified siRNAs that contain internal azobenzene derivative spacers for selective and tunable photocontrol of activity
Short interfering RNAs (siRNAs) are biopolymers that are used as post-transcriptional gene regulators and as a natural endogenous defense against attack from viruses. These molecules hold a great deal of interest as pharmaceuticals for the knockdown of unregulated genes, such as in cancers. Through …
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Elucidating the role of effector caspases in immune development using lentiviral RNAi
… silencing by the introduction of double stranded RNAs, known as short interfering RNAs (siRNAs), through a mechanism that is well conserved across many species. While this technique has been used successfully in tissue culture experiments, our studies focused on extending the use of RNAi into …
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Differential expression of small RNAs in soybean
Small RNAs are non-protein coding RNAs that regulate gene expression in plants primarily by cleaving mRNA. Two major classes of small non-coding RNAs are microRNAs (miRNAs) and short interfering RNAs (siRNAs) of 21-24 nucleotides. I selected some small RNAs that show tissue specificity based on …
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Specificity and mechanism of microRNAs in the regulation of gene expression
… of endogenous, small RNA species termed microRNAs (miRNAs). The establishment of a mammalian tissue culture system has allowed for investigation of both the mechanism and specificity of miRNA-directed translational repression. The term RNAi can be used to encompass a wide variety of gene …
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Exploring the specificity and mechanisms of siRNA-mediated gene silencing in mammalian cells
Complementary short interfering RNAs (siRNAs) are routinely used to knockdown gene expression. siRNAs bind to their target sequence and guide transcript cleavage and subsequent degradation. This type of silencing is associated with equivalent levels of RNA and protein knockdown. siRNA-mediated …
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Genomics and regulatory functions of microRNAs and small silencing RNAs in Arabidopsis thaliana
… transitions. In plants, endogenous small RNAs consist of two broad classes, the ~21-nucleotide (nt) microRNAs (miRNAs) and the diverse ~22-24-nt trans-acting and heterochromatic short interfering RNAs (siRNAs). In order to more extensively characterize the small RNA landscape in plants and …
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The role of tetraspanin pretein CD63 in humna immunodeficiency virus type 1 infection and replication
… in cells was decreased by RNA interference using short interfering RNAs (siRNA). Inhibition of HIV replication was demonstrated in macrophages following CD63-specific siRNA treatment. The inhibition effect was also shown when the anti-CD63 antibody treatment was delayed 12 hours after HIV …
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Structural variation at soybean loci regulating small RNAs and seed color
… color is determined by a specific class of small RNAs known as short- interfering RNAs (siRNAs). The dominant ii allele of the I (inhibitor) locus is composed of an inverted-repeat cluster of six chalcone synthase (CHS) genes on chromosome 8 whose unique arrangement generates CHS siRNAs that …
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The synthesis and in vitro evaluation of siRNAs modified at the backbone with a novel triazole-based internucleotide linkage and abasic alkyl-chain linkages of varying length
For over a decade, the use of double-stranded short interfering RNAs (siRNAs) to silence the expression of genes associated with disease at the translational level has gained much attention. Using a highly conserved endogenous pathway within cells, siRNA technology displays a high degree of target …
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Mouse Models of Inherited Skin Diseases
… strategy for disrupting mutant KRT9 allele with short interfering RNAs (siRNAs) was explored as a potential therapeutic approach. This led to the development and in vitro validation of potent mutation- and gene-specific siRNA inhibitors. <br/>Despite its importance in EPPK, little is known about …
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Functional analysis of the Argonaute family proteins during early embryonic and germ cell development in the mouse
… through their association with small non-coding RNAs. Specifically in mammals, microRNAs (miRNA) and short interfering RNAs (siRNAs) bind to Ago-like subclade of the Argonaute family to regulate gene expression, while Piwi-interacting RNAs (piRNAs) associate with PIWI proteins to silence …
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Exploiting the Conflict Within: Examining Tripartite Interactions for Development of Next Generation Mosquito Microbiome Vector Control Strategies.
… that infection lead to dramatic increases in short interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs). Additionally, 17 host miRNAs had altered levels across multiple time points. Finally, the mosquito RNAi response to ZIKV targeted the NS5 region, while ZIKV in response produced …
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The synthesis and in vivo evaluation of chemically modified siRNAs that contain red-shifted internal azobenzene derivative spacers for selective and tunable photocontrol of activity without UV light
Short interfering RNAs (siRNAs) are biopolymers that are used for post-transcriptional gene regulation and act as endogenous defenses against attack from viruses and dsRNA parasites. The siRNA field is making advances for use as pharmaceuticals and biomolecular research tools for the study of gene …
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Advancing and expanding siRNA and saRNA therapeutics applications through chemical modifications
Oligonucleotides are short strands of DNA or RNA that are used to treat complex diseases like cancer and rare genetic diseases. They rely on biological pathways in our body to work. Two pathways that are important to this study are gene silencing and activation. Short interfering RNAs (siRNAs) …
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Context Dependent Regulation of Dicer Function During Oocyte-to-Embryo Transition
… enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, disease progression, fertility, and cancer. Interestingly, …
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The conjugation and evaluation of hydrophobic small molecules to trazole-linked siRNAs
… nucleic acid-based therapies, such as short interfering RNAs (siRNAs), have been developed to aid in the regulation of proteins. SiRNAs serve as substrates for the highly conserved RNA interference (RNAi) pathway, which gives rise to their high degree of mRNA target specificity and …
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