Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"shake flask"”.
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Process development and scale-up from shake flask to fermenter of suspended and immobilized aerobic microorganisms
… grown on lactic acid. Respiration measurement in shake flasks is introduced as a screening method to characterize the behavior of microorganisms exposed to stress factors such as carbon starvation, anaerobic conditions, organic acids, osmolarity and pH. It is evaluated and compared with the …
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Preliminary investigation of growth and antimicrobial production by streptomyces polyantibioticus : from shake flask to stirred tank bioreactor
… compounds from submerged cultures of SPRT in shake flasks, and scale-up of the process into a laboratory stirred tank bioreactor (STR) was done in the present study. The work focused on obtaining growth kinetics and suitable operating conditions for cultivation. Characterisation of the growth …
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The process intensification of biological hydrogen production by Escherichia coli HD701
… coli HD701 using a two‐phase process (growth in shake flasks, followed by hydrogen production within a bioreactor). Ideally a commercial process would need to be in a single vessel (bioreactor), which therefore resulted in this investigation of the scale‐up of twophase fermentations to 5 L …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… conditions (20°C, 0.5 mM, IPTG and pH 7) using shake flask cultivation followed by bioreactor conditions (20°C, 0.5 mM, IPTG and pH 7) in batch fermentation. The immobilized cells onto GO using shake flask cultivation exhibit about 3.8-fold increase in xylanase production (0.4821 U/ml) or equal …
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The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum
… Thirty-nine fungal strains were screened under shake flask conditions. The major criteria used in evaluating these strains were, the yield of γLA per unit volume (g/l) and γLA as a percentage of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial …
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Overproduction of xylose isomerase in recombinant Escherichia coli
… cell metabolism and inducible enzyme activity in shake flask cultures. E. coli strain LE392 (pRK248/pTXI-l) performed the best with respect to nearly all of the parameters tested. The stability of the overproducing plasmids was tested in prolonged serial shake flask cultures. Segregational …
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Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production
… be produced with high levels of expression in shake-flask culture. Furthermore, preliminary studies have also shown that the molecular weight of the amidase was approximately 35kDa, and that it acts optimally at a temperature and pH of 50°C and 7.2 respectively.
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Process development for ethanol production from food waste
… and fermentation (SSF) was investigated in shake-flask cultures, with scale-up of the best performing conditions to a 5-L bioreactor. The resource assessment identified two sites within Gauteng province with sufficient capacity to produce an estimated 1 464 500- and 247 860-Lethanol/annum, …
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Bioleaching of chalcopyrite
… (ATCC 19859), which has been carried out in shake flasks (250 ml) and a 4-litre stirred tank bioreactor. The effects of experimental factors such as initial pH, particle size, pulp density and shake flask speed have been studied in shake flasks by employing cell suspensions in the …
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The effect of simazine on nitrification and the decomposition of simazine by soil microorganisms
… effect on the growth of Nitrosomonas europaea in shake flask culture, but did inhibit the growth of Nitrobacter agilis. The addition of yeast extract to the culture medium did not reverse the inhibition. Simazine did not inhibit the respiration of N. agilis even at a 100ppm concentration. Five …
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Microbial synthesis of 3,4-dihydroxybutyric acid, 3-hydroxybutyrolactone and other 3-hydroxyalkanoic acids
… yield and titers of the order of 1 g/L at the shake flask scale. Overcoming repression of the glyoxylate shunt and independent control of the glycolate and 3HA pathway enzyme expression using two orthogonal expression systems was critical for product synthesis. Additionally, a study of the …
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Continuous Microbial Production of 2,3-Butylene Glycol From Cheese Whey Permeate
… fermentations. Fermentation scale-up from shake flask to pilot plant was accomplished. Large scale fermentations required nascent acid neutralization by NaOH for high glycol yields. Acid neutralization plus acetate supplementation to the growth medium produced high fermentation efficiencies …
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Genetic engineering oleaginous yeasts for improved lipid production
… and 9.5±1.3 g/L lipid from 70 g/L xylose in shake-flask experiments. Next, in order to further improve lipid production in R. toruloides, we explored more genetic engineering routes. We found over-expressing malic enzyme and stearoyl CoA desaturase could improve lipid production 23% and 28 % …
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A central composite design to investigate antibody fragment production by Pichia pastoris
… This experimental design involved running shake flask culture at 15 different experimental conditions with duplicates. Optical density (OD600), dry cell weight (DCW), and BCA Protein Assays were done on each experiment. Mathematical models in terms of these parameters' effects and their …
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Improved Polyhydroxyalkanoate Production from Selected Volatile Fatty Acids using Cupriavidus necator
… involved the following investigations using shake flasks and 5 litre based batch fermentations:<br/><br/>Shake flask investigations determined that the temperature of 30oC and a nutrient medium, resulted in the highest growth of bacteria. A feeding strategy of the substrate (VFAs – acetic …
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Tryptophanase Regulatory Mechanisms in Escherichia coli
… pulse. This work has combined conventional shake-flask culture approaches with single-cell analysis to investigate tryptophanase expression and activity across all *E. coli* growth phases. From these studies a hypothesis has emerged that the indole pulse is triggered, at least in part, by …
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Effects of adding acid plant sulfur on heap leaching
… columns used to simulate mine heap conditions. Shake flask tests were also run to observe the potential effects with increased aeration. The study concluded that there were no harmful effects observed towards leaching with the addition of the waste sulfur. This project has been useful to the …
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Physiological studies on bacterial fermentations using multi-parameter flow cytometry
… an agitated bioreactor. It was demonstrated in shake-flask culture that the cells could be grown to moderate cell densities (OD600nm 7 25) whilst producing measurable levels of antibody fragment. Despite being described in a patent which claims invention of a 100 % effective repression system …
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Protein and Pathway Engineering for Biosynthesis or Aromatic Compounds
… with an estimated yield of 0.7 g/L under the shake flask condition and 20 g/L using a continuous fermentation for 5 days. In vitro assay revealed that PhlD catalyzed the synthesis of phloroglucinol from three molecules of malonyl-CoA, and exhibited broad substrate specificity, which was …
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