Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"shRNA screening"”.
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In vivo pool-based shRNA screens to identify modulators of disease progression in hematopoietic malignancies
shRNA screens have been very effective in identifying novel cancer genes in mammalian cells, but they have primarily been limited to in vitro applications in tumor cell lines. Whereas in vivo retroviral mutagenesis screens typically identify gain-of-function alterations in positive selection …
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In vivo shRNA screen reveals PRMT5 as a key regulator in glioblastoma
… and demonstrate its feasibility for in vivo shRNA screening. We describe the results of parallel in vivo and in vitro shRNA screens to uncover epigenetic regulators with oncogenic functions and chemosensitizing potential in GBM. From these screens, the protein arginine methyltransferase PRMT5 …
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TARGETING METABOLIC PLASTICITY OF GLIOBLASTOMA TUMOR INITIATING CELLS TO RESTORE SENSITIVITY TO LSD1-DIRECTED THERAPY
… By performing a high-throughput synthetic lethal shRNA screening, we discovered that Post-GPI Attachment to Proteins 1 (PGAP1), a protein involved in the quality check of proteins’ folding in the ER, is a mediator of such resistance. Targeting PGAP1 in LSD1-resistant TICs sensitizes refractory …
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Integrating Functional Genomics with Systems Biology to Discover Drivers and Therapeutic Targets of Human Malignancies
Genome-wide RNAi screening has emerged as a powerful tool for loss-of-function studies that may lead to therapeutic target discovery for human malignancies in the era of personalized medicine. However, due to high false-positive and false-negative rates arising from noise of high-throughput …
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Optimization of molecular tools for high-throughput genetic screening
Forward genetic screening allows for the identification of any genes important for a particular biological process or phenotype. While the power of this approach is broadly agreed on, the efficacy of currently available tools limits the strength of conclusions drawn from these experiments. This …
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Exploring genetic interactions with G-quadruplex structures
… genes were silenced via short-hairpin RNAs (shRNAs) whilst being treated with either PDS or PhenDC3, two independent G4-stabilising small molecules. I explored gene deficiencies that enhance cell death (sensitisation) or provide a growth advantage (resistance) in the presence of these …