Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"serum-free media"”.
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Developing Serum-Free Media Via Bioprocessing For Cultivated Seafood Products
… however, the conventional use of Fetal Bovine Serum (FBS) in culture media restricts its utilization at an industrial level. FBS is effective but has many limitations: unethical animal extraction, high demand and low supply, poorly defined ingredients, variable performance, and high cost that …
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Serum-free media development using black soldier fly protein isolate and hydrolysate for cultivated meat
… challenge: the high cost of cell culture media, primarily due to the use of Fetal Bovine Serum (FBS). Substituting serum with protein hydrolysates reduces the production expense of cultivated meat products and promotes establishing a sustainable food system. This study explores black …
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Development of monoclonal antibody cell culture processes and antibody characterisation
… Upstream studies examined the effects of media formulations and seeding density studies on antibody expression. While serum-supplemented media supported the growth of CHO-DP12 cells, the serum-derived impurities and inconsistent antibody expression limited the production of anti-IL-8 mAbs. …
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Investigating aneuploidy's role in cancer cell fitness under various conditions of stress
… various culture conditions, including regular media, serum-free media, cytotoxic drug-containing media, and hypoxia. We found that aneuploid cells proliferated better than diploid cells under all but standard culture conditions. Moreover, regardless of growth condition, we found that aneuploid …
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A high-content multiplexed screening platform for the evaluation and manipulation of force and fatigue of adult derived skeletal muscle myotubes in defined serum-free medium
… First, was to develop a protocol utilizing serum-free media formulations and defined plating and culture techniques to create functional in vitro myotubes derived from adult skeletal muscle satellite cells. The second was to manipulate the inherent muscle parameters such as force output and …
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Decellularized Apium graveolens Scaffold for Cell Culture and Guided Alignment of C2C12 Murine Myoblast
… believed to be dependent on four pillars: animal free scaffolds, serum free media, bioreactors and cell lines or stem cells. In 2014, decellularized plant tissue emerged as an animal free scaffold for three-dimensional cell culture. Despite the fact that plant parenchyma provides a relatively …
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An in Vitro Study of Cellular Cardiomyoplasty: Structural and Functional Interactions of Non-cardiomyocytes and Cardiomyocytes
… in vitro. Specifically, we conditioned a defined serum-free media (control media) for 24 hours in the presence of non-cardiac cells and assessed electrophysiological properties. Our results indicate that (1) that paracrine factors secreted by cardiac fibroblasts could contribute to the progression …
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New sustainable models of open innovation to accelerate technology development in cellular agriculture
… to the translational development of cell lines, serum-free media, cell-scaffolds, and bioreactor designs with regulatory and market assessment efforts, require new models for industry collaboration. The Open-Innovation Network Map was used to prioritize key collaboration networks to address the …
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Mammalian Cell Line Development Platform for Recombinant Protein Production: Expanding the Protein Expression Toolbox for Research and Drug Discovery Applications
… to a high density in commercially available serum-free media with a doubling time of less than twenty four hours. A clone isolation strategy was used to pick the top clone expressing high levels of recombinant protein. Using the highest expressing clone, we developed a high yielding …
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Studies of CD36 interacting with fatty acids, oxidized low-density lipoprotein, and the cellular plasma membrane
… or diffusion in control experiments with protein-free phospholipid bilayers. To gain new insights into the physiological relevance of FA binding to CD36, we applied surface plasmon resonance (SPR) to quantify FA and oxLDL binding to the ectodomain of CD36. Structurally distinct FA [saturated, …
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Is my eye okay with OK lens?: comparative in vitro study depicting the effects of Orthokeratology (OK) lens treatment on the physiology of human corneal epithelial cells
… for the correction of refractive error. OK mediates its effect by reshaping the cornea curvature with the use of a specially designed rigid contact lens, in order to correct the focusing of the image onto the retina, thereby implying direct contact with the epithelial layer of the cornea …
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Development of exosome-based anti-cancer therapeutics
… grown in medium supplemented with foetal bovine serum (FBS) that contains naturally occurring exosomes. Bovine-derived exosomes can cause artefacts and interfere with interpretation of results. The aim of this study was to investigate the release of exosomes from human liver cancer cell line …
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Investigating the role of TRPC3 in the biogenesis of extracellular vesicles
… that can be transferred between cells. EV-mediated communication contributes to a wide range of physiological and pathological processes including cancer progression, yet the molecular mechanisms underlying their formation and release remain incompletely characterised. Elevated …
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Bioengineering of Heterogenous Glioblastoma Multiforme Microenvironment
… organoid studies rely on growing them in serum-free media, resulting in sphere formation. However, this conventional method is not scalable and often fails in recapitulating inter- and intratumor heterogeneity. Also, the conventional method is not ideal to produce adequate quantities of …
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CHCHD2 and the tumor microenvironment in glioblastoma
… form of primary brain tumor in adults, with a median survival time of only 14.6 months. GBM tumors present with chemo- and radio-resistance and rapid, diffuse invasion, making complete surgical resection impossible and resulting in nearly universal recurrence. While investigating the genomic …
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Expression and role of the extracellular matrix protein tenascin in ovarian cancer
… lines were studied. TN levels in conditioned media were measured by ELISA. "Basal" levels of TN secretion were determined by culturing cells in serum-free media; under these conditions the ovarian fibroblasts secreted levels of TN over 100 fold greater than the epithelial cells. RT-PCR data …
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Characterisation of dysregulated proteins in macrophages infected with Mycobacterium smegmatis focusing on matrix metalloproteases and their effectors
… Differentiated THP-1 monocytes were incubated in serum-free media with or without bacilli. Thereafter, the secretome and lysate were harvested at different time points. The activity of MMPs was analysed by zymography. The activity of MMP-1 and MMP-9 were specifically determined using an MMP-1 …
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Development of a novel bioreactor and systems for suspension cell culture in biopharmaceutical production
… cells into single-cell suspension culture in serum-free media by treating them with an anti-cancer drug, Puromycin amino nucleoside (PAN). The absence of a supporting surface for cell growth (e.g. microcarriers) and serum-free conditions are expected to reduce the cost of manufacturing and to …