Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 82 for “"serum-free"”.
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Osteochondral allograft preservation in a serum-free chemically-defined media
… storage. Our work showed that storage in a serum-free chemically-defined media at 37[degrees]C can maintain OCAs \ better than the current tissue banking protocol for storage.
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Baculovirus stability in serum-free lyophilized and wet storage conditions
… to maintain virus stability, and fetal bovine serum is commonly used in the storage solution. In an effort to lower transportation and storage costs, a storage formulation that can effectively store the baculovirus in above frozen temperatures without the use of FBS would be beneficial. In this …
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Developing Serum-Free Media Via Bioprocessing For Cultivated Seafood Products
… however, the conventional use of Fetal Bovine Serum (FBS) in culture media restricts its utilization at an industrial level. FBS is effective but has many limitations: unethical animal extraction, high demand and low supply, poorly defined ingredients, variable performance, and high cost that …
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Serum-free media development using black soldier fly protein isolate and hydrolysate for cultivated meat
… media, primarily due to the use of Fetal Bovine Serum (FBS). Substituting serum with protein hydrolysates reduces the production expense of cultivated meat products and promotes establishing a sustainable food system. This study explores black soldier fly larvae (Hermetia illucens) as an emerging …
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A high-content multiplexed screening platform for the evaluation and manipulation of force and fatigue of adult derived skeletal muscle myotubes in defined serum-free medium
… First, was to develop a protocol utilizing serum-free media formulations and defined plating and culture techniques to create functional in vitro myotubes derived from adult skeletal muscle satellite cells. The second was to manipulate the inherent muscle parameters such as force output and …
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DEVELOPMENT OF A CULTURE SYSTEM FOR BOVINE LUTEAL CELLS: EXAMINATION OF LUTEOTROPIC AND LUTEOLYTIC MECHANISMS
… cultured in Ham's F-12 medium with or without serum. The serum-free medium was supplemented with insulin, transferrin and hydrocortisone. Addition of LH to the serum-containing medium did not increase P(,4) production. When the luteal cells were cultured in serum-free medium, LH produced an …
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Studies on the Regulation of Hepatic Hmg Coenzyme a Reductase and the Production of Anti-Hmg-Coenzyme a Reductase Monoclonal Antibodies
… Chang liver cells have been adapted to growth in serum-free chemically defined Higuchi's medium for the purpose of studying the regulation of HMG-CoA reductase.
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Studies on the fatty acid composition of human serum with special reference to the influence of hormones and other agents
A modified technique for separation of serum free fatty acids (FFA), t riglycerides, phospholipids and cholesterol esters by adsorption chromatography on a system of two columns, silicic acid and Florisil, has been elaborated for preparing samples for gas-liquid chromatographic analysis of serum …
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Diversity among monocyte derived stromal cells
… derived fibrocytes can be differentiated in both serum-containing and serum-free environments and we wanted to study the relationship between these two fibrocytes; which potentially could be involved at different time points at a wound healing site. To investigate the relationship between …
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Eviscerated corneas as tissue source for ex vivo expansion of limbal epithelial cells on platelet-rich plasma gels
… with DMEM and fifty controls were placed in serum free DMEM alone. Donor patients received systemic antibiotics prior to evisceration. Results: Confluent epithelium in mono-layers could be cultured when donor limbal biopsies were placed in a mixture of A-PRP culture medium and DMEM. No growth …
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Analysis of the roles of ligand and DNA binding in estrogen receptor action
… Hamster Ovary cells which were grown under serum-free conditions, suggested that commercial base media may contain traces of estrogenic compounds. Additional studies demonstrated that transcriptional synergy by ER is strongly dependent on both cell-type and ER concentration, and is likely …
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IN-VITRO VASCULARIZED TISSUE EQUIVALENTS FROM HUMAN EMBRYONIC STEM CELL-DERIVED ENDOTHELIAL AND VASCULAR SMOOTH MUSCLE CELLS
… tissue equivalents (VTE). Under feeder-free and chemically-defined conditions, we demonstrate efficient differentiation of hESCs into vascular progenitor populations using sequential modulation of Wnt, FGF, BMP and VEGF signaling pathways. Further, we demonstrate the differentiation of …
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Differential membrane-type matrix metalloproteinase expression in phenotypically defined breast cancer cell lines: Comparison of MT-MMP expression in environmentally-challenged 2D monolayer cultures and 3D multicellular tumour spheroids
… including hypoxia and nutrient deprivation (serum-free induced autophagy) were measured in MCF-7 monolayer cultures and MCTS models using immunofluorescence (IF), immunohistochemistry (IHC) and western blot (WB). MT1-MMP expression was rapidly and irreversibly up-regulated in MCF-7 breast …
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Opioid inhibition of the growth of the transplantable androgen-responsive (AR) Shionogi mouse mammary carcinoma (SC115)
… incubated in medium containing 5% fetal bovine serum(FBS), 2% dextran charcoal-treated FBS (DCTFBS), or seurm-free medium, with or without different concentrations of dihydrotestosterone (DHT), hydrocortisone (HC), 178-estradiol(E2), basic fibroblast growth factor (bFGF), epidermal growth factor …
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Biology of adult human normal and leukaemia CD133+ stem cells
… drug resistance in elderly AML patients. A novel serum-free culture system for inducing neural differentiation of human adult bone marrow and foetal liver CD 133+ stem cells in vitro was demonstrated. Following only two weeks' culture of selected bone marrow CD 133+ cells in serum-free medium …
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The Role of Glutamine and Mevalonate Pathway In Affecting The Stemness and Viability of Glioblastoma Stem Cells
… vitro, </em>glioblastoma stem cells cultured in serum-free medium form self-renewing neurospheres, express the neural stem marker CD133, and are highly tumorigenic. On the other hand, in the presence of fetal bovine serum (FBS), the glioblastoma stem cells undergo differentiation.</p> <p>In this …
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Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
… responses to the mitogenic effects of serum growth factors. Myoblasts expressing the human $\alpha$5 subunit differentiate in the absence of serum while differentiation of untransfected and $\alpha$6 transfected myoblasts is insensitive to serum concentration. Addition of individual, …
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Long-term expansion, genomic stability, in vivo safety and immunogenicity of adult human pancreas organoids
… efficiently long-term in a chemically defined, serum-free medium. The aim of this project was to generate a 3D culture system for long-term expansion of human pancreas ductal cells as hPOs to serve as the basis for studies of human pancreas ductal epithelium, exocrine pancreatic diseases and the …
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Effects of HOXB4 downstream targets on the haemopoietic differentiation of pluripotent stem cells
… and human ESCs. We first developed a defined, serum and feeder-free protocol to test the effects of these secreted factors on haemopoietic differentiation. This defined protocol allowed us to compare the haemopoietic potential of mouse ESCs with the recently derived epiblast stem cells …
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