Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"serotyping"”.
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Monoclonal antibodies against Neisseria Gonorrhoeae: serotyping and diagnostic applications
… monoclonal antibody coagglutination reagents for serotyping gonococci according to Protein I variation between strains; (ii) to determine if these or similar monoclonal antibodies could be used in an immunological method for the non -cultural detection of N. gonorrhoeae; and (iii) to produce …
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Monoclonal and phage-displayed antibodies as lipopolysaccharide serotyping reagents and their use in typing colony variants of Vibrio vulnificus
… (LPS) O-serovars have previously been used as a serotyping system in epidemiological studies. Here is the first report of two additional MAbs capable of recognizing two new LPS serovars (O6 and O7), further increasing the number of V. vulnificus that can be typed. Analysis of V. vulnificus …
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The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli
… on farm B, the source farm of pigs to farm A. Serotyping and flaA typing were carried out to study the epidemiology of the isolates. Serotyping identified 0:24 (11/61) as the most frequent serotype isolated, followed by 0:5 (7/61). Common serotypes 0:48, 0:54 and 0:59 were identified in strains …
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ΚΑΤΑΝΟΜΗ ΒΙΟΤΥΠΩΝ ΚΑΙ ΟΡΟΤΥΠΩΝ ESCHERICHIA COLI ΣΕ ΚΛΙΝΙΚΑ ΥΛΙΚΑ. ΣΥΣΧΕΤΙΣΗ ΦΑΙΝΟΤΥΠΩΝ ΑΝΤΙΣΤΑΣΕΩΣ ΚΑΙ ΟΡΟΤΥΠΩΝ
… TO BIOTYPES, 25 BIOCHEMICAL REACTIONS WERE USED. SEROTYPING WAS PROCESSED WITH 36 MONOVALENT ANTISERA WHILE SUSCEPTIBILITY TO ANTIBIOTICS WAS CARRIED OUT WITH THE USE OF STANDARDIZED 13 SINGLE DISC DIFFUSION METHOD. OUT OF 249 STRAINS, 179 WERE CLASSIFIED TO 23 BIOTYPES. THE MOST FREQUENT WERE 2 …
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Similaridade genotípica de Salmonella enterica isolada de animais silvestres e exóticos em cativeiro em Ohio, EUA
… Susceptibility testing by disk diffusion method, serotyping and genotyping by macrorestriction of chromosomal DNA. Salmonella was detected in 56/225 (25%) of fecal samples, 6/56 (11%) of environmental samples and 6/38 (16%) of food samples. This pathogen was more frequently found in fecal samples …
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Studies on pneumococci isolated from children in the Sheffield area.
… of vaccination in high risk patients. Serotyping of strains by co-agglutination of sensitised protein-A particles, was quicker and as sensitive as crossed immuno-electrophoresis (CIE), without requiring the complex CIE equipment. The increased and more active growth obtained under …
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Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus
… further classified by type of toxin produced, serotyping, and phage typing. I have used random amplified polymorphic DNA (RAPD) fingerprinting to determine the phenetic relationships among 31 pathogenic and 14 nonpathogenic strains of B. sphaericus. DNA Bands in agarose gel migrating the same …
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Fate and Dissemination of Salmonella Reading in Market-age Turkeys at Processing using Oral Gavage Challenge Model
… and streaked on XLT-4 agar for recovery and serotyping of SR colonies. SR could not be recovered from stifle joint 14 days P.I. Skin samples showed the highest incidence of SR recovery (80 %) 14 PI, followed by crop (75 %); LS (67.5 %); lungs (60 %); and ceca (57.5 %). The organ with the …
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An evaluation of DNA fingerprinting methods for subtyping Salmonella
… years. Traditional typing methods, including serotyping and phage typing, have often not adequately discriminated strains, nor have they always identified virulent or antibiotic resistant strains. In a literature review, DNA-based methods, including plasmid analysis, restriction fragment …
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Identification of major histocompatibility complex haplotypes in goldfish, Carassius auratus
… The utility of chicken anti-chicken reagents in serotyping of fish was demonstrated. Use of the traditional approach to conducting hemagglutination assays limited the number of assays executed because of the amount of blood required. In order to minimize the sample volumes required, antibody …
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Structural analysis of capsular polysaccharides produced by some emerging Klebsiella pneumoniae serotypes
… serotypes, that have been classified through serotyping. Furthermore, there are additional K-locus (KL) serotypes that have been recently identified using genotyping. This project involved the characterisation and structural elucidation of the CPS repeating unit (RU) structures, known as …
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Investigations of enterotoxigenic E. Coli (ETEC) intestinal colonization in neonatal mice and human shedding of panchol, a new live attenuated oral cholera vaccine
… of serial dilutions of fecal samples. Serotyping of shed PanChol was carried out using anti-Ogawa and anti-Inaba antisera. RESULTS: There were marked differences in ETEC small intestinal colonization in different mouse strains. Outbred CD-1 suckling mice only colonized with a 107 dose. …
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Phenotypic and genotypic characterisation of invasive Streptococcus pneumoniae expressing atypical capsular types
… surveillance program, exhibiting atypical serotyping results. Methods: A convenient sample of 68 atypical isolates were selected for the purpose of this study. Pneumococci were isolated and characterized using conventional microbiological laboratory techniques. Confirmed pneumococci were …
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Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction
Traditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and …
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Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
Traditional serologic assays of Actinobacillus pleuropeumoniae often have problems with cross-reactivity. To avoid the complications of antibody-antigen reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. Primers specific to the conserved …
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A pan-genome wide association study to identify genes associated with invasive Streptococcus pneumoniae
… fluid of patients (652 samples). In-silico serotyping identified 56 serotypes in the cohort and statistical analysis showed that despite the vaccination, the prevalence of serotypes 1 and 12F increased amongst patients. Genomes were assembled, and a reference pan-genome for all strains was …
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Isolation of Bacterial pathogens from dead- in- shell Chicken embryos from local Hatcheries
… forty three (43)were Staphylococcus aureus. Serotyping of Escherichia coli revealed that six (6) cultures were serotype 08:k47, five (5) were serotype 09:k28, two (2) belonged to serotype 032/33:k- and one (1) each were serotype 088:k-, 016:k- and 0101 :k-. Out of seventy cultures of …
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Isolation of Bacterial pathogens from dead- in- shell Chicken embryos from local Hatcheries
… forty three (43)were Staphylococcus aureus. Serotyping of Escherichia coli revealed that six (6) cultures were serotype 08:k47, five (5) were serotype 09:k28, two (2) belonged to serotype 032/33:k- and one (1) each were serotype 088:k-, 016:k- and 0101 :k-. Out of seventy cultures of …
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A molecular epidemiological analysis of meningococcal isolates within Scotland 1972-1998
… However, phenotypic methods for serogrouping, serotyping and serosubtyping meningococci, although providing good strain information, can lead to endemic strains appearing identical using these methods when they are in fact quite different. More recently methods have been developed to further …