Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"serine proteases"”.
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Characterization of subtilisin-like serine proteases in trichomonas vaginalis
… found worldwide. In this study, subtilisin-like serine proteases (subtilases) were examined for their putative role in cell viability. Published data of other eukaryotic protozoan parasites confirm the importance of subtilases for adhesion and invasion. Serine protease inhibitor assays show that …
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The role of midgut serine proteases in Aedes aegypti vector competence
… through the alimentary tract may exploit serine proteases in the midgut to enhance vector infection. Recent genetic and biochemical experiments have demonstrated that dengue virus type 2 (DENV-2) may require proteolytic processing by midgut trypsins to efficiently infect Aedes aegypti. …
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Molecular biology studies on the extracellular serine proteases of Vibrio alginolyticus
… bacterium that produces several extracellular serine proteases and a collagenase during the stationary growth phase. The aim of this study was to investigate alkaline serine protease production by this organism, and to attempt the cloning and expression of a V.alginolyticus protease gene in …
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Studies on the properties of two intracellular serine proteases from Bacillus subtilis
Intracellular serine protease-1 (ISP-1) from Bacillus subtilis had been previously purified to homogeneity. The purified protease (Mr = 31,000) had undergone processing to remove 17 to 20 amino-terminal amino acid residues. Then observations and a number of other studies led to the proposal that …
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Guanidino-aryl substituted enol lactones: Selective and potent mechanism-based inhibitors of trypsin-like serine proteases
… examined as potential inhibitors of trypsin-like serine proteases. The first class of protio and iodo enol lactones had either a 4-guanidino phenyl substituent (3-(4-guanidinophenyl)-6-methylidene tetrahydro-2-pyranone (1) and 3-(4-guanidinophenyl)-6-(E)-iodomethylidene tetrahydro-2-pyranone (2)) …
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Design and Use of Fluorescent Assays to Study Plasminogen Activator and Other Serine Proteases Involved in Biological Control
In order to study the role of serine proteases in the wide variety of physiological functions which they serve, sensitive and quantitative assays need to be devised. Because the xanthene dyes rhodamine and fluorescein are among the most fluorescent compounds known and, as such, can be detected at …
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HOST DEFENSE PEPTIDES OF THE COAGULATION SYSTEM AND THEIR THERAPEUTIC POTENTIAL
… relationships of small HDPs derived from serine proteases. In paper I and II, structural features, governing the antimicrobial activity of peptides derived from the C-terminal region of human serine proteases were identified. Moreover the potential of these peptides to modulate …
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Identification and characterisation of proteases in Mycobacterium tuberculosis
… to cause extensive tissue necrosis. Exported proteases are commonly associated with virulence in bacterial pathogens, yet their role in Mycobacterium tuberculosis has virtually not been studied. Preliminary experiments showed M. tuberculosis culture filtrates contained a proteolytic activity …
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Discovery Of Inactive E* Conformations In Thrombin And Other Vitamin K- Dependent Clotting Proteases
Serine proteases of the chymotrypsin family play important roles in the regulation and function of numerous biological processes including digestion, blood coagulation, fibrinolysis, development, fertilization, apoptosis and immunity. For many of these proteases, activity unfolds when a zymogen is …
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1-Acylamido boronic acids and Difluoroborane analogs of amino acids: Synthesis and biological activity of transition state analogs for Chymotrypsin and Elastase (inhibition, esters)
… as ""transition state"" inhibitors of the serine proteases (alpha)-chymotrypsin and elastase. These inhibitors were designed to resemble N-acyl phenylalanine, phenylglycine (as chymotrypsin inhibitors), alanine, valine, and isoleucine (as elastase inhibitors) and have boron sybstituted for …
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DETECTION OF SECRETED PROTEASES AND A MEMBRANE PROTEASE IN PATHOGENIC ACANTHAMOEBA CULBERTSONI
… in the literature that secreted Acanthamoeba proteases have the ability to degrade components of the extracellular matrix. The role of amoebic proteases in mediating the attachment and invasion processes is not entirely understood. Initial experiments conducted in the present study revealed …
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Decomposition of Proteins into Functionally and Evolutionarily Independent Cooperative Units
… - the TonB dependent receptors and the S1A serine proteases. The TonB dependent receptor family members are membrane bound siderophore transporters. Ligand binding on the extracellular side of the transporter transmits a signal to the periplasmic side where another protein (TonB) provides …
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The synthesis of aminophosphonic acid derivatives & related compounds
… that irreversibly inhibit a wide variety of serine proteases; as structural units in butyloxycarbonyl protected phosphonate esters that complex with alpha-lytic proteases; and as structural units in peptidyl phosphonate diphenyl 9 10 esters which powerfully inhibit the enzyme thrombin. …
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Metal complexes of non-heme ligands: biological applications
… reductive activation of O<sub>2</sub>, whereas serine proteases trypsin and chymotrypsin were inactivated by ferryls (single turnover), and by an iron complex in presence of biologically relevant oxidant, H<sub>2</sub>O<sub>2</sub> (catalytic conditions). Analysis of the CA–I inactivation …
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Synthetic, Sulfated, Lignin-Based Anticoagulants
… found to be potent inhibitors of a number of serine proteases via allosteric mechanisms targeting heparin binding sites. Herein, we describe the creation of synthetic sulfated β-O4 lignin (SbO4L) polymer, which is more homogenous compared to previous lignins with respect to its inter-monomeric …
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Activity of Analogs of Anticancer Drugs on the Serine Protease Enzymes Subtilisin and Chymotrypsin
… activity. Chymotrypsin and subtilisin are serine proteases that have a histidine residue in the active site. We are investigating the inhibition of the digestive enzymes chymotrypsin and subtilisin by analogs of the anticancer drug cisplatin and trying to discern trends in the inhibition as …
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INVESTIGATION OF THE MIALOME OF IXODES SCAPULARIS REPLETE TICKS AND UNDERSTANDING THE POPULATION GENOMIC STRUCTURE OF IXODES PACIFICUS TICKS
… In Chapter 3, function of three highly expressed serine proteases (identified in chapter 2) in blood digestion was determined. In Chapter 3, we showed that the serine proteases are indeed active in blood digestion in fully engorged females. These findings advance our understanding of tick …
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Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus
… production of an extracellular collagenase and serine proteases by Vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37°C and by lack of oxygen. V. alginolyticus had identical growth rates at 30 and 37°C. Aeration did not affect the growth rate of …
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Design and synthesis of small-molecule inhibitors as anti-malarial and anti-serpin agents
… activator inhibitor-1 (PAI-1), an endogenous serine proteases inhibitor implicated in a wide range of biological processes. Malaria is one of the top global health threats, and there is a great need for developing effective new chemotherapies. PAI-1 is a major component in the regulation of …
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Generation of novel small molecules as inhibitors of plasminogen activator inhibitor-1
… activator inhibitor (PAI-1) is a member of the serine protease inhibitor (serpin) family of proteins, which inhibits certain serine proteases, such as tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), and thus is a primary regulator of fibrinolysis, the …
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