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Showing 1 to 20 of 74 for “"sephadex"”.
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Sephadex G-25 and Charcoal Separation of Mouse Growth Inhibitors in Soybeans from Trypsin Inhibitors
<p>Rising costs have changes man’s ideals and eating habits. Plant ingredients, because of their relatively low costs, have been increasingly used as a substitute for animal proteins. If these plant ingredients could be directly utilized by man, this would be a more efficient use of energy and …
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ΣΥΜΒΟΛΗ ΣΤΗΝ ΠΡΟΛΗΨΗ ΤΟΥ ΠΥΡΗΝΙΚΟΥ ΙΚΤΕΡΟΥ. ΑΞΙΑ ΤΗΣ ΜΕΘΟΔΟΥ ΤΗΣ SEPHADEX G25 ΧΡΩΜΑΤΟΓΡΑΦΙΑΣ ΓΙΑ ΤΗΝ ΑΠΩΤΕΡΗ ΕΞΕΛΙΞΗ ΙΚΤΕΡΙΚΩΝ ΝΕΟΓΝΩΝ
… TO INVESTIGATE WHETHER THE SIMPLE AND RAPID SEPHADEX GEL FILTRATION COULD RECOGNISE JAUNDICED NEWBORNS WITH NON ALBUMIN BOUND BILIRUBIN WHICH ARE PRONE TO MANIFEST LATER SIGNS OF BILIRUBIN ENCEPHALOPATHY. WE TESTED 78 BABIES (72%) OF 108 BABIES WITH JAUNDICE WITHOUT ANY OTHER MAJOR PERINATAL …
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Novel Compounds From Methanogens: Characterization of Component B of the Methylreductase System and Mobile Factor
… by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for …
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Investigation of purification procedures to isolate rat mitochondrial ẟ - aminolaevulinic acid synthetase
… 5-20% (w/v) polyethylene glycol precipation, CM-sephadex chromatography, Sephadex G-100 filtration, and electrophoresis. 3. Attempts to duplicate previously reported purifications of ALV-Synthetase by use of affinity chromatography were unsuccessful. 4. Isoelectric focusing gave no clearly useful …
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Delipidation Treatments for Large-Scale Protein Purification Processing
… of a whey/TNBP mixture with pre-swollen Sephadex LH-20 or hydroxyalkoxypropyl dextran (HAPD) beads at 4 C for 24 hours removed 67 + 2 % (0.645 mg triglycerides/ml Sephadex LH-20) and 71 o + 1 % (0.628 mg triglycerides/ml HAPD) of the triglycerides present in the skimmed transgenic whey, …
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Partial Characterization of Animal Growth Inhibitors from Soybeans
… soybean meal was separated into 5 fractions on a Sephadex G-25 column in efforts to isolate the animal growth inhibitors present soybeans. Each fraction was added to the diet of growing mice and growth inhibition (GI) activity was determined by comparing their_ growth rates to g1"'owth rates …
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Identification of soybean, Glycine max (L.) Merr., cytokinins
… purified by polyvinylpyrrolidone (PVP), paper, Sephadex LH-20 and high performance liquid chromatography. The fractions separated on the basis of polarity into polar, moderately polar and relatively non-polar groups, compared to zeatin. The polar fraction was the single most bioactive fraction …
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A study of the binding of anti-aggregatory substances to platelet bound plasma proteins.
… or ligand. This led to the development of a Sephadex batch method to be used in conjunction with fluorescence. The concentration of free ligand was measured directly in the Sephadex gel. Factors which effect the fluorescence from the Sephadex bed were extensively studied. To show the …
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Effects of the Removal of Adherent and Phagocytic Cells on the Spleen Cell Lymphoproliferative Response of Tumor Bearing Mice
… normal mice. Following macrophage removal by Sephadex G-10 columns an d carbonyl iron ingestion, there were no significant changes in the SI values for spleen cells from the #51 cell-injected mice. In contrast, spleen cells from mice injected with H238 cells, a herpes virus-transformed cell …
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Studies of the Host Response and the Induction of Immunosuppression in Murine Malaria
… (E), detergent-insoluble components (N), and Sephadex G-200 eluate fractions (H(,1), D(,1)).
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A Ca²⁺-activated proteinase in chicken skeletal muscle
… been partially purified by DEAE-cellulose and Sephadex G-150 chromatography. CAP from embryonic muscle shows similar properties to the corresponding enzyme from adult tissue with respect to calcium dependence (maximum activity at 1.0 rnM Ca²⁺), pH optimum (7.2) and sensitivity to proteinase …
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Isolation and Characterization of Fatty Acid Binding Protein From Lactating Bovine Mammary Gland
… the cytosolic fraction by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on DEAE Sephadex A-25. FABP was eluted from the ion exchange column by .1 M NaCl. Molecular weight of FABP was estimated to be 12,000 by SDS polyacrylamide gel electrophoresis (PAGE). Two …
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Vitamin A and glycoproteins of rat corneal epithelium
… by a stepwise column chromatography on DEAE-Sephadex A-50. A specific "peak S" glycopeptide eluted between 0.35 N and 0.42 N LiCl solution was found to be the most decreased (approximately by 50%) glycopeptide component by vitamin A deficiency when a new continuous gradient column …
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The effect of vitamin A on plasma glycoproteins
… amounts of protein and fractionated on DEAE-Sephadex A-50. There was a consistent decrease m radioactivity observed m what appeared to be the alpha₁ peak in vitamin A-deficiency. This depression was on the order of 30%, when normal and deficient peak totals were compared. This effect appeared …
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The metabolism of adenosine nucleotides in thoracic muscle mitochondria from the American cockroach
… fractionation and column chromatography on G-75 sephadex. Some properties of the enzyme were determined. These include: a pH optimum of 5.8, an optimum Mg concentration of 3 x 10⁻³ M, a substrate specificity for ADP, and an equilibrium constant of 0.44. In addition, Km and Ki values were …
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Biochemical characterization of nitrate transport at the plasma membrane of plant cells using isolated vesicles
… by aqueous column chromatography in Sephadex G-10, allows for the recovery of about 80% of the initial radioactivity as $\sp{36}$ClO$\sb3\sp{-}$. This radiolabeled anion was used as a tracer for nitrate in membrane transport studies with isolated plasma membrane vesicles.
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Der agglutinierende, antifungale Faktor aus den Knollen von Cyperus esculentus: Optimierung der Reinigung und weitere Untersuchungen zur Hemmung des Wachstums phytopathogener Pilze
… pH 6.4 mit anschließender Gelfiltration an Sephadex G-25 als vorteilhaft. Diese Methode lieferte eine Ausbeute von 81 % und einen Reinigungsfaktor von 143. Erst nach Erhöhung der Ionenstärke konnte der CEF durch hydrophobe Interaktionschromatographie weiter gereinigt werden. Diese Methode …
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Halogenorganische Verbindungen in Umweltkompartimenten
… wurden Amberlite XAD-8, Duolite A-7 und Sephadex G 25. Nur eine kleine Fraktion besteht aus unpolaren, nicht ionischen und kleinen halogenierten Komponenten. Gerade diese Komponenten werden durch Gaschromatographie leicht nachgewiesen. Dieses ist der Grund für die Bestimmungslücke …
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Purification of Vitamin D Binding Protein and Its Role in the Uptake of 25-Hydroxyvitamin D by Cultured Kidney Cells (Mdck Cells, Tissue Culture, Dbp)
… steps: chromatography on DEAE cellulose and DEAE Sephadex, followed by chromatofocusing. The procedure represents a shorter purification scheme than those previously reported. Antibodies to DBP were produced in rabbits and coupled to cyanogen bromide-activated Sepharose 4B. With this antibody …
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… by ion exchange on DEAE-cellulose and QAE-Sephadex, affinity chromatography on CBZ-phe-TETA-Sepharose-4B, gel filtration chromatography on either Sephadex G-75 or TSK G3000 SW using HPLC system and finally Polyacrylamide gel electrophoresis. For convenience the protease was assigned a …
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