Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"sensor kinase"”.
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PhoQ: Structural and Mechanistic Investigations into an Important Bacterial Sensor Kinase
… alongside the first full-length model of a sensor kinase.
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Structural and Functional Studies of Sensor Kinase RetS from Pseudomonas aeruginosa and Peptidoglycan Hydrolase SleB from Bacillus anthracis
Part I: Signaling Role of the Sensor Kinase RetS in Biofilm formation Regulation of Pseudomonas aeruginosa-<br />The opportunistic human pathogen Pseudomonas aeruginosa causes both acute and chronic infections in predisposed individuals. Acute infections require a functional Type Three Secretion …
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Characterization of the Activation of the FlgSR Two-Component System in Campylobacter Jejuni
… regulatory system comprised of the FlgS sensor kinase and the FlgR response regulator to activate transcription of the middle and late sigma54-dependent flagellar genes. The FlgR response regulator is an NtrC-like protein that can be divided into three domains: an N-terminal domain that …
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Gene Regulation by a Novel Two-Component System Conserved Among Gammaproteobacteria
… events between two proteins, a membrane bound sensor kinase and a cytoplasmic response regulator. Activation of a two-component system can be caused by biological or environmental stimuli, resulting in altered gene expression. A conserved two-component system was recently discovered in …
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Characterization of two component systems of Acinetobacter baumannii.
… that typically consist of a membrane bound sensor kinase and a cognate response regulator. Bacterial TCSs play an important role in the regulation of adaptation to different environmental conditions. Five TCSs in A. baumannii have been characterized; however, there are a number of putative …
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The role of bacterial interspecies communication in polymicrobial infection of the cystic fibrosis lung
… that these responses require the P. aeruginosa sensor kinase PA1396. The work in this thesis aims of further elucidate the influence and mechanism of DSF signalling on P. aeruginosa and examine the role that such interspecies signalling play in infection of the CF airway. Next generation …
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Genetic and environmental regulation of virulence genes in Salmonella enterica serovar Typhimurium.
… utilization of propanediol and ethanolamine. The sensor kinase BarA and its response regulator SirA control levels of CsrA indirectly through the expression of an untranslated RNA CsrB, which binds CsrA. SirA regulates expression of Salmonella virulence genes and is required for Salmonella …
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Signaling between Two Sensor Kinases Controls Biofilms and Host Colonization in a Bacterial Symbiont
… to survive. To do this, cells utilize sensory circuits, which function to connect environmental stimuli to an intracellular response. One common sensory pathway utilized by bacteria is two-component signaling (TCS), composed of an environmental sensor (the sensor kinase, SK) and a …
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Investigation of global regulators influencing styrene metabolism and bioplastic synthesis in Pseudomonas putida CA-3
… of the mini-Tn5 library revealed a gacS sensor kinase gene disruption resulting in the loss of PHA accumulation capacity in P. putida CA-3. Subsequent SDS-PAGE protein analyses of the wild type and gacS mutant strains identified post-transcriptional control of phaC1 synthase as a key …
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The Characterization of Quorum Sensing E. Coli Regulators E,F, and G (Qse EFG) and Their Role in Pathogenesis
… signals are often recognized by bacterial sensor kinases. In response to cognate signals, sensor kinases autophosphorylate and transfer the phosphate to a response regulator. The regulator then binds downstream genes to regulate transcription. This pathway for flagellation and motility, …
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The Functional Characterization of the Quorum Sensing E. Coli Regulators B and C in EHEC
… [1]. Here, we show that QseC, the membrane-bound sensor kinase, can autophosphorylate itself in response to AI-3 or epinephrine/norepinephrine and then transfer this phosphate to the response regulator, QseB. The autophosphorylation of QseC is not affected by the addition of autoinducer-2 or …
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Alternative regulation of the alginate algD operon by an activated AlgB in nonmucoid Pseudomonas aeruginosa is dependent on Sigma 54
… σ54. However, neither σ54 nor the cognate sensor kinase (KinB) of AlgB are required for algD expression in such mucoid strains. I hypothesized that KinB-phosphorylated AlgB must play some role in gene regulation, and so I sought to construct a constitutively active AlgB that simulated …
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The Role of Host Hormones and Metabolites in the Regulation of Virulence in Enterohemorrhagic Escherichia coli (EHEC)
… transcriptome studies. The bacterial adrenergic kinases QseC and QseE both respond to epinephrine to regulate the LEE PI positively and negatively respectively. We also demonstrated for the first time that co-incubation with epinephrine increases the formation of AE lesions, and that QseC and …
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N-acylhomoserine lactone regulation of adhesion and biofilm differentiation in Serratia marcescens MG1
… regulated genes resulted in reduced adhesion; a sensor kinase gene (rssA), a type I transporter gene (lipB), bsmA and bsmB. These four genes were found to effect the expression of type I fimbriae which is proposed to be the adhesin affecting C4-HSL regulated adhesion. While C4-HSL is not involved …
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Novel genes required for proper cell envelope function in the plant symbiont Rhizobium leguminosarum
… novel phenotypes associated with mutation of the sensor kinase chvG that regulates cell envelope structure. Collectively, the results of this study have identified new cell envelope genes that are critical for proper envelope function, and contributed to the development of a new model for cell …
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Identifying Novel Factors Involved in Biofilm Formation by Vibrio Fischeri
… the syp polysaccharide locus, as well as the sensor kinase RscS and the response regulators SypG, required for syp transcription, and SypE, a negative regulator of biofilm formation. However, I predicted that other factors were also involved in biofilm formation. Therefore, my dissertation …
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Going Beyond Phosphorylation: Acetylphosphate-Mediated Ran Polymerase Acetylation and Caper Phosphorylation Co-Regulate Transcription of Escherichia Coli Stress-Responsive Gene cpxP
… via a transfer of phosphoryl groups from the sensory protein know as sensor kinase (SK) to the output protein known as response regulator (RR).</p><p>In addition to this constant input of extracellular information, bacterial cells also monitor their intracellular environment, as among other …
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The role of ATM responding to DNA damage induced by Xrcc1 deficiency during oligodendrocyte genesis
… Atm-/-) to determine the function of DNA damage sensor kinase Atm upon persistent DNA damage condition by Xrcc1 inactivation during brain development. Xrcc1 is a scaffolding protein that is recruited to DNA single strand break site with several interacting proteins such as PARP1, DNA polymeraseβ …
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Regulation of amylovoran biosynthesis in Erwinia amylovora
… 2) Comparative characterization of RcsC sensor kinase showed that the sensor domain of RcsC was important for the regulation of bacterial virulence; 3) novel genes regulated by RcsB and RcsC were identified by microarray assays. Third, a conserved hypothetical gene amyR from E. amylovora …
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Studies Toward the Syntheses of Antiarrythmic, Anti-Virulence, and Anticancer Small Molecules
… regulator C (QseC), a membrane-bound histidine sensor kinase, mediates the expression of various virulence genes in Gram-negative bacteria such as Escherichia coli (EHEC), Salmonella typhimurium, and Francisella tularensis which are pathogenic to humans. Therefore, QseC is a potential target of …
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