Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"selenocysteine"”.
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Synthesis of Selenium-Alkylated Selenocysteine Derivatives and Their Use in Native Chemical Ligation, Expressed Protein Ligation and the Synthesis of Dehydropeptides
… an improved, scalable synthesis of Fmoc-phenylselenocysteine is reported. The amino acid was coupled to a model peptide on the solid phase. It was demonstrated that the selenide could be oxidatively eliminated to give a dehydropeptide while still bound to the solid resin. In addition, the …
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Mechanisms of Translational Control
… tRNASec bound to eukaryotic elongation factor selenocysteine (eEFSec). Our results reveal a mechanism of Sec UGA recoding in eukaryotes that differs fundamentally from its prokaryotic counterpart. Next, we investigated the consequences of inefficient Sec incorporation on human health. …
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Investigating Potential Therapies to Decrease the Rate of Cystine Stone Growth in Slc3a1-/- Mice
… interventions – sulforaphane, methyl selenocysteine, homocysteine, tiopronin, TPEN and a zinc-supplemented diet– were also examined for their efficacy in reducing the rate of cystine stone growth. µCT analysis revealed stone growth proceeds linearly. Sulforaphane and TPEN …
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Multi-species genome-wide CRISPR screens identify conserved suppressors of cold-induced cell death
… (BHK-21) cell line, and uncover GPX4 and related selenocysteine incorporation genes as important for protection against cold-induced cell death. Using genetic knockdowns, along with overexpression of GPX4, we confirm our findings and demonstrate that levels of GPX4 may be limiting in K562 cells, …
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Selenotrisulfide Derivative Of Alpha-lipoic Acid: Evaluation In A Cell Culture Model For Potential Use As A Topical Antioxidant
… cells. It is incorporated in the form of selenocysteine into selenoenzymes such as glutathione peroxidase and thioredoxin reductase, and is absolutely required for activity. Thioredoxin reductase is necessary for reduction of oxidized thioredoxin and therefore plays a major role in …
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Acetylation regulates Thioredoxin Reductase activity and oligomerization
… the 21st genetically encoded amino acid, selenocysteine (Sec). There were multiple experimentally identified TrxR acetylation sites with an unknown effect on TrxR activity. My thesis tested the hypothesis that programmed protein acetylation will enhance the activity of TrxR1. I used …
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Reactions of Platinum(II) Compounds with Selenium Containing Amino Acids
… amino acids including selenomethionine and selenocysteine, new research is ongoing to see what types of products can be formed from these amino acids. Our research reacts [Pt(Met-<em>S</em>,<em>N</em>)Cl<sub>2</sub>] <sup>2+</sup> with selenomethionine to determine what types of products are …
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Investigating [NiFe]-hydrogenases in γ-Proteobacteria
… previously characterised FHL complexes have selenocysteine-containing formate dehydrogenases. Using genetic and biochemical approaches it was shown that the [NiFe]-hydrogenase and a formate dehydrogenase were vital for H2 production by P. atrosepticum. Using plant infection assays it was also …
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Investigations of metalloenzymes involved in bacterial natural product biosynthesis
… of the C-terminal cysteine of TglACys by selenocysteine yielded a potent inhibitor of TglHI activity. Based on these results, several short, synthetically accessible peptide fragments were identified as candidate substrates and inhibitors for use in further mechanistic and structural …
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Isotopic studies of selenium in environmental samples.
… organically bound Se as selenomethionine and selenocysteine was found to be around 100% efficient and the use of a microwave oven increased the number of samples which could be processed. A sequential extraction procedure was used to determine the soil fractions with which Se was associated. …