Global ETD Search
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Showing 1 to 12 of 12 for “"selected reaction monitoring"”.
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Global and targeted proteomics in Arabidopsis thaliana: A study of secondary metabolism and phytohormone signaling
… peptide derivitization, and those that do not. Selected-reaction monitoring (SRM) for targeted proteomics, and data-independent acquisition (MSE) was also evaluated. In addition to assessing the capabilities of these technologies, we then applied them to the context of uncovering new insights …
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DEVELOPMENT OF A LC/MS/MS ENZYME METHOD FOR N8 - ACETYLSPERMIDINE MEASUREMENTS IN ENZYME ASSAYS
… y is the peak area of the fragment-ion SRM (selected reaction monitoring: <em>m/z</em>: 188/114) chromatograms from N<sup>8</sup> -acetylspermindine and x is the concentration of N<sup>8</sup> - acetylspermindine. Acetylation of spermidine (SPD) and N<sup>8</sup>-acetylspermidine (N8AcSPD) …
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Characterization of the specificity and functions of the protein phosphatase Cdc14
… phosphorylation. I demonstrate that label-free selected reaction monitoring (SRM) mass spectrometry is comparable to conventional assays for measuring reaction rates and steady-state kinetic parameters of phosphatases and kinases. Furthermore, I demonstrate that this method can be employed to …
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Proteomic-based Signature of Brain-related Proteins as Novel Candidate Biomarkers for Alzheimer’s Disease Diagnosis
… identified in the human CSF proteome were selected for development of multiplex mass spectrometry-based assay. Candidate biomarkers were then evaluated in AD patients with different spectrum of disease severity and cognitively healthy individuals (in total 154 subjects). This exploratory …
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A new mass spectrometric assay of N8-acetylspermidine deacetylase and partial purification of the enzyme
… on mass spectrometric detection of a specific reaction product following sample introduction by flow injection, was shown to have a sensitivity of smaller than 1 micromolar and typical RSD of 3-10%. The linear range for analyte was from 1 μM to 100 μM, with R<sup>2</sup> > 0.992. The new assay …
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A flexible and versatile framework for statistical design and analysis of quantitative mass spectrometry-based proteomic experiments
… and 10 experimental datasets from targeted Selected Reaction Monitoring (SRM), Data-Dependent Acquisition (DDA or shotgun), and Data-Independent Acquisition (DIA or SWATH-MS), where signals were extracted with multiple signal processing tools. We implemented the proposed method in the …
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Proteomics in Nasal Lavage Fluids from Persulfate Challenged Hairdressers
… of 246 NLF proteins was developed using selected reaction monitoring (SRM). Also, a strategy for identification of multiple oxidative modifications was developed and applied on proteins oxidized in vitro by persulfate. SRM assays targeting the oxidized peptides were developed. NLF samples …
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Insights into vitamin B12 production, acquisition, and use by marine microbes
… part of phytoplankton molecular physiology. Selected reaction monitoring mass spectrometry was used to measure the abundance of CBA 1 and methionine synthase proteins in diatoms cultures, revealing distinct protein abundance patterns as a function ofB12 availability. These peptide …
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Changes in tear film biochemistry in end of day ocular discomfort with and without contact lenses
… tears was achieved using a novel method based on selected reaction monitoring (SRM) mass spectrometry. The concentrations of prolactin-induced protein, proline rich protein 4, resolvin D1 and C3a are reported for the first time. Concentrations of lysozyme and lactoferrin, secretory phospholipase …
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Insights into vitamin B₁₂ production, acquisition, and use by marine microbes
… part of phytoplankton molecular physiology. Selected reaction monitoring mass spectrometry was used to measure the abundance of CBA1 and methionine synthase proteins in diatoms cultures, revealing distinct protein abundance patterns as a function of B₁₂ availability. These peptide …
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The Development and Implementation of a TMT-SRM Assay for the Validation of Candidate Biomarkers of Alzheimer’s Disease
… rapid, high quality, quantitative assays. Selected reaction monitoring (SRM) has emerged as an accurate and precise method for targeted protein selection through signature peptide measurement in highly complex mixtures such as plasma. Quantitative SRM-based assays are an attractive …
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Nuclear PTEN Regulates Thymidylate Biosynthesis and Cellular Sensitivity to Antifolate Treatment
… A polar metabolomics profiling platform using selected reaction monitoring with the 5500 QTRAP hybrid triple quadrupole mass spectrometer, created by Dr John Asara, was utilized for metabolomics data generation. Using LC MS/MS for polar metabolite profiling the platform is a single normal phase …