Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"seeding density"”.
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Diamond seeding process for the heterogenous integration of high quality diamond on semiconductors
… nucleation enhancement steps through the diamond seeding process are generally required. In this work, an effort is made to improve seeding density, nucleation process, growth kinetics, film morphology, quality as well as material thermal properties of diamond film on different semiconductors. The …
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Syncytiotrophoblast formation is influenced by genes involved in lipid biology
… trophoblast fusion in vitro. Anecdotally, cell seeding density has been reported to influence cell fusion. This hypothesis was systematically tested with three seeding densities, assessed at four time points. These experiments confirmed that increased seeding density causes increased cell …
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Cell seeding and proliferation simulations to plan experiments of multicellular osteogenic systems
Osteogenesis in vivo involves changes to cell density, morphology, cell-cell contacts, gene expression, and proliferation; however, the coordination of these changes is unknown. Specifically, cells undergo a morphological switch; they are proliferative, pluripotent, elongated cells early in …
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Inhibiting metastatic potential: an analysis of migratory body formation in chondrosarcoma
… into a fresh cell culture plate. Different cell seeding densities were used in 6 well-plates to optimise the production of MBs. 5000 cells/cm2 was found to be the optimum seeding density to produce the maximum number of MBs in SW1353. EGF and FGF were used to further optimise the formation of …
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Enhancing Mesenchymal Stem Cell Chondrogenesis for Cartilage Tissue Engineering
… were applied. Specifically, the effects of cell seeding density, media formulation and mechanical stimulation were examined with respect to functional growth. Transient application of TGF-β3 improved the compressive properties of MSC-laden constructs, but only when constructs were formed at a …
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Near wall high resolution particle image velocimetry and data reconstruction for high speed flows
… Any TRDPIV measurement requires uniform spatial seeding density, but time-resolved measurements require uniform temporal seeding density as well. To this end, a high pressure particle generator was developed. This advancement enhanced current capability beyond what was previously attainable. …
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Differentiation of mouse embryonic stem cells into insulin-producing cells: optimization to enhance the overall differentiation efficiency
… body culture conditions, digested cells seeding density and various coatings required for differentiation to optimize a previously established protocol to enhance the overall differentiation efficiency. Moreover, a three-dimensional in vitro collagen tissue culture system was prepared to …
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Stream Discharge Measurement Using A Large-Scale Paticle Image Velocimetry Prototype
… and flow regime (Froude number), and particle seeding density. Camera angle was optimum around 15 degrees, with larger camera angles producing more error due to image distortion. Conditions at high Froude numbers likely produced out-of-plane displacement losses due to surface disturbances. Low …
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Development of monoclonal antibody cell culture processes and antibody characterisation
… examined the effects of media formulations and seeding density studies on antibody expression. While serum-supplemented media supported the growth of CHO-DP12 cells, the serum-derived impurities and inconsistent antibody expression limited the production of anti-IL-8 mAbs. In contrast, CHO-NIST …
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RESTORATION OF NATIVE PLANT COMMUNITIES AFTER ROAD DECOMMISSIONING: EFFECT OF SEED MIX AND SOIL PROPERTIES ON VEGETATIVE ESTABLISHMENT
… (native vs. nonnative), species diversity, and seeding density on vegetative establishment, and 3) impact of overstory canopy and coarse woody debris on revegetation success on recently decommissioned roads. Total vegetative cover declined by 60% one-year after decommissioning, with nonnative …
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RESTORATION OF NATIVE PLANT COMMUNITIES AFTER ROAD DECOMMISSIONING: EFFECT OF SEED MIX AND SOIL PROPERTIES ON VEGETATIVE ESTABLISHMENT
… (native vs. nonnative), species diversity, and seeding density on vegetative establishment, and 3) impact of overstory canopy and coarse woody debris on revegetation success on recently decommissioned roads. Total vegetative cover declined by 60% one-year after decommissioning, with nonnative …
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Lung tissue engineering : in vitro synthesis of lung tissue from neonatal and fetal rat lung cells cultured in a three-dimensional collagen matrix
… was supplemented with 10% fetal bovine serum. A seeding density between 1 to 5 million cells per sponge sample was used. Histology studies were performed at termination periods of 2, 14, and 28 days. This paper describes the in vitro formation and long-term maintenance of alveolar-like structures …
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Soybean growth and yield response to interplant competition relief in various plant density environments
… sized plants respond to different plant density environments, or how plants respond to competition relief at various developmental stages. Research was conducted in Urbana, Illinois to examine yield component and growth variability by plant density, interplant competition, and cohort …
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Proliferation and morphology of pre-osteoblast cells on 25 – 1000 micron diameter patterned features on polyacrylamide hydrogels
… cell proliferation, maturation, morphology, and density all play a role of in osteogenesis, most studies of controlled cell differentiation either focus proliferation and maturation in large cell colonies or morphology of single cells. However, large cell colonies are difficult to examine because …
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The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)
… The 10,000 of hUC-MSCs at passage 3, seeding in 60 mm petri dish were grown in constant ‘direct exposure’ (DE) of 21.6 mT SMF, alongside with ‘indirect exposure’ (IE) and ‘negative control’ (NC) groups. The growth kinetic experiments in the current study exhibited exponential growth at …
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Optimization and Translation of MSC-Based Hyaluronic Acid Hydrogels for Cartilage Repair
… maturation by optimizing macromer and MSC seeding density. The beneficial effects of dynamic compressive loading, high MSC density, and continuous mixing (orbital shaker) resulted in equilibrium modulus values over 1 MPa, well in range of native tissue. While compressive properties are …
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Modulation of cell morphology and function using synthetic biodegradable polymers
… Both polymer composition and initial cell seeding density affected cell growth. PLGA films were formulated with copolymer ratios of 50:50 and 75:25 and thickness levels of 10 and 100 mum. In vitro degradation of these thin films in simulated body fluid was characterized in terms of mass …
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Cardiac tissue engineering : bioreactor cultivation parameters
… parameters, including (I) cell source, (2) cell seeding density, (3) cell seeding vessel, and (4) tissue culture bioreactor on structure and function of engineered cardiac cell-polymer constructs were studied. Advantages of seeding mammalian cells at high densities (6-Sx 106 cells/Smm diameter x …
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Untersuchung zur Proliferation neocorticaler und hippokampaler Neurone in vitro
Diese Arbeit untersucht die Proliferation neocorticaler und hippokampaler Neurone in vitro. Ziel war, neocorticale Primärkulturen der neugeborenen Ratte nach Zelltypen aufzutrennen und in reineren Kulturen zu vermehren. Besonderes Interesse galt der Anreicherung neuronaler Präkursoren und der …
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