Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"seeded aggregation"”.
-
The Mechanisms of Tau Entry to the Cytosol
… in a ‘prion-like’ manner, resulting in templated aggregation of native tau in recipient neurons. Interactions between tau assemblies, surface receptor LRP1 and heparan sulphate proteoglycans promote the uptake of tau assemblies to membrane-bound vesicles. A subsequent escape from these vesicles is …
-
The prion-like properties of assembled human alpha-synuclein
… alpha-synuclein by studying the pathways of seeded aggregation, and to identify the species of alpha-synuclein responsible. I present evidence, using a HEK 293T cell model, that filamentous protein was the most seed-potent form of alpha-synuclein. Recombinant aggregated protein, aggregated …
-
Tau Aggregation, Conformational Selection, and Inhibition
… from 3R and 4R MAP2 slow nucleation and block seeded aggregation of 4R Tau protein. Also, as few as a single MAP2 molecule bind to and cap the 4R Tau fibril end. This could account for the inhibition via disruption of the ability of Tau to subsequently bind to the capped fibril end. MAP2 …
-
Structural studies of the in vitro assembly of tau and α-synuclein amyloids
… divided into three parts. First, I describe the seeded assembly of recombinant α-synuclein filaments with seeds from brains with Multiple System Atrophy (MSA), and show that seeded assembly does not necessarily replicate the structures of the seeds. The results in this section have important …
-
Lessons from Tau RT–QuIC: Structure, Kinetics, and Drug Discovery
The aggregation of the microtubule–associated protein tau into filamentous inclusions is a hallmark of various neurodegenerative diseases collectively known as tauopathies. This group encompasses over 20 clinicopathological conditions, including Alzheimer’s disease (AD), which accounts for about …
-
Single Molecule Fluorescence Studies of Prions and Prion-like Proteins
… of individual murine PrP aggregates from seeded aggregation in vitro. From fluorescence imaging of individual PrP aggregates on the coverslip surface, elongation and fragmentation of the PrP assemblies have been directly observed. PrP elongation occurs via a structural conversion from a …
-
Investigating tau propagation in situ
… trafficking, intercellular transfer and seeded aggregation of assembled tau. However, the underlying molecular mechanisms are poorly understood. The goal of my thesis was to investigate tau propagation in situ. I focussed on characterising the ultrastructural context of assembled tau …
-
The role of the type-I interferon response in the aggregation of tau protein
… in neurons and/or glia. In AD, tau protein aggregation is accompanied by extracellular plaques of β-amyloid (Aβ) protein, protein pathologies which feature alongside prominent neuroinflammation. The innate immune response, the first line of defence against foreign pathogens, is emerging as a …