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Showing 1 to 15 of 15 for “"sedimentation velocity"”.
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Albumin Adsorption: Inferences of Protein Interactions Measured by Sedimentation both Between Species and Induced by Denaturing
… (ZATLLS) that has been used to measure the sedimentation velocity of several different materials in solution. In this case, bovine serum albumin (BSA) will be adsorbed onto polystyrene particles and the particle settling velocity determined. The settling solution viscosity and density will …
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Nonideality in high concentration solutions
… from the nonideality of the background solution; Sedimentation velocity can be used in place of sedimentation equilibrium and provide similar insights into interactions in complex solutions.</p><p>The proximity energy framework was found to account for analytical ultracentrifugation data gathered …
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Structural, biochemical, and inhibition studies of proline biosynthetic enzymes
… To fill this gap, we report crystallographic, sedimentation velocity, and kinetics data for human PYCR1. Structures of binary complexes of PYCR1 with NADPH or proline determined at 1.9 A resolution provide insight into cofactor and substrate recognition. We see NADPH bound to the Rossmann fold, …
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Free solution electrophoresis measurements and their theoretical relationships to net protein valence
… behavior entirely to changes in charge. Sedimentation velocity and dynamic light scattering were used to verify that the hydrodynamic radius was constant for a given series of charge mutants. Furthermore, this work compares the model predictions of Debye-Huckel-Henry (DHH), Booth, and the …
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Studies on the Proteolytic Activation of the Membrane Enzyme, Pyruvate Oxidase
… dodecyl sulfate polyacrylamide gels. However, sedimentation velocity studies combined with quasi-elastic light scattering results show that the proteolysis has no effect on the structure of the native tetramer. Amino-terminal analysis of native and protease-activated pyruvate oxidase indicates …
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A PROTEIN IN SEARCH OF FUNCTION: NIPSNAP1 IN MITOCHONDRIAL BRANCHED-CHAIN AMINO ACID METABOLON, BRAIN AND APOPTOSIS
… antibodies against the recombinant protein. Sedimentation velocity shows that NIPSNAP1 is a homotetramer. It is localized in the mitochondrial matrix where the BCAA enzymes are present and is imported inside mitochondria in an energy-dependent manner. NIPSNAP1 can associate with the E2 …
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SAXS and X-ray Crystallography Studies of the Cellulosome from Clostridium thermocellum
… and the X-module of another scaffoldin molecule. Sedimentation velocity experiments indicate dimerization also occurs in solution. We have carried out the crystallization and structural analysis of a heterotrimeric complex containing the CohI9—X-DocII:CohII complex bound to the type-I dockerin …
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Decreased Triiodothyronine Binding to the Hepatic Nuclear Thyroid Hormone Receptor in the Diabetic Mouse
… nonspecific binding. Isoelectric focusing and sedimentation velocity of the receptor preparation did not demonstrate any differences between control and diabetic mice. Comparison of the level of saturation of the nuclear T<sub>3</sub> receptor in the diabetic mouse to other obese syndromes …
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Structural Studies of Recombinant Human β-casein proteins
… native protein.</p> <p>Results from turbidity, sedimentation velocity and laser light scattering studies show that m+4 has a monomeric molecular weight of around 22 kDa, which is very close to that of its native counterpart. In low salt buffer, it behaves similarly to the native OP protein in …
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The purification and characterization of phosphoenolpyruvate carboxykinase
… yield (48%). The enzyme appears homogeneous in sedimentation velocity experiments and has a sedimentation coefficient (s₂₀⁰,w) of 5.21 S. Its molecular weight, determined by sedimentation equilibrium is 73,300. The ultraviolet and visible absorption spectra of the enzyme revealed no …
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Study of prothrombin activation by taipan snake venom using light beating spectroscopy
… 67x 1 2cm /sec at the respective concentrations. Sedimentation coefficients determined from sedimentation velocity experiments were 5, 37S and 6. 7S at 2 mg/ml and 5 mg /ml. These were higher than the reported values of 4. 8S and 5. 2S for the corresponding concentrations, which could be …
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Cooperative Assembly of Terminase and Integration Host Factor at the Packaging Initiation Site of Bacteriophage Lambda
… of protein-DNA interactions. Furthermore, sedimentation equilibrium studies demonstrate that while terminase assembles in the absence of IHF as a dimer on a 274 bp DNA substrate inclusive of the entire cos site, in the presence of IHF a nucleoprotein complex of mass consistent with five …
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Biochemical characterisation of a novel vaccine candidate against Trypanosoma vivax, the cell surface glycoprotein IFX
… were verified to be IFX by mass spectrometry. Sedimentation velocity analysis demonstrated that the total fraction of IFX extracellular domain was forming multiple stable oligomers, but that the isolated lower glycoform displayed fast reversible self-association. The third results chapter …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
The global conformations of DNA polymerase I from Thermus aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then …
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Development of MWL-AUC / CCD-C-AUC / SLS-AUC detectors for the analytical ultracentrifuge
… physicochemical parameters. For example, sedimentation equilibrium experiments can lead to the study of pure thermodynamics. For complex mixtures, AUC is the main method that can analyze the system. Interactions between molecules can be studied at different concentrations without …