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Showing 1 to 12 of 12 for “"sedimentation equilibrium"”.
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Nonideality in high concentration solutions
… from the nonideality of the background solution; Sedimentation velocity can be used in place of sedimentation equilibrium and provide similar insights into interactions in complex solutions.</p><p>The proximity energy framework was found to account for analytical ultracentrifugation data gathered …
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Purification of nicotinamide adenine dinucleotide phosphate- specific glutamate dehydrogenase from Chlorella sorokiniana and partial characterization of its physical, kinetic, and immunological properties
… weight of the native enzyme, as determined by sedimentation equilibrium, Sephadex G-200 gel filtration and gradient polyacrylamide gel electrophoresis, indicate that the native enzyme may be elliptical in shape. The amino acid composition of the enzyme is high in glycine, glutamate, and …
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Tuning polymeric chiral surfactants for enhanced enantiomeric recognition in micellar electrokinetic chromatography
… The molecular weights were determined by use of sedimentation equilibrium mode in AUC. Examination of the separation data obtained from MEKC experiments indicated that fractionated polymeric surfactants yielded a better chiral separation. In addition, increase in molecular weight of the …
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Cooperative Assembly of Terminase and Integration Host Factor at the Packaging Initiation Site of Bacteriophage Lambda
… of protein-DNA interactions. Furthermore, sedimentation equilibrium studies demonstrate that while terminase assembles in the absence of IHF as a dimer on a 274 bp DNA substrate inclusive of the entire cos site, in the presence of IHF a nucleoprotein complex of mass consistent with five …
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The purification and characterization of phosphoenolpyruvate carboxykinase
… yield (48%). The enzyme appears homogeneous in sedimentation velocity experiments and has a sedimentation coefficient (s₂₀⁰,w) of 5.21 S. Its molecular weight, determined by sedimentation equilibrium is 73,300. The ultraviolet and visible absorption spectra of the enzyme revealed no …
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The Arrangement of the Disulfide Bonds in a γG Immunoglobulin Molecule
… molecule was 154,000 ± 8,000, as measured by sedimentation equilibrium. The component light (molecular weight 22,500) and heavy (molecular weight 51,600) chains were isolated in high yield by gel filtration after reduction and alkylation under mild conditions. Amino terminal analyses and the …
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Characterization of structure and enzymic activity of endo-l, 4-β-D-glucanases purified from Trichoderma viride
… of 37,200, 52,000 and 49,000 as determined by sedimentation equilibrium. The three endoglucanases each contain mannose, galactose, glucose and glucosamine. Endoglucanase II contains significantly less carbohydrate (4.5% by weight) than Endoglucanases III and IV which contain 15.0% and 15.2%, …
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Study of prothrombin activation by taipan snake venom using light beating spectroscopy
… 67x 1 2cm /sec at the respective concentrations. Sedimentation coefficients determined from sedimentation velocity experiments were 5, 37S and 6. 7S at 2 mg/ml and 5 mg /ml. These were higher than the reported values of 4. 8S and 5. 2S for the corresponding concentrations, which could be …
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Biochemical investigation into the toxic salivary secretion of the tick, Ornithodoros savignyi Audouin (1827)
… and was found to be homogeneous according to sedimentation equilibrium centrifugation. The molecular mass, determined by the latter method was found to be 9680. The amino acid composition of the fraction revealed, with a few exceptions, similarity with that of hyaluronidase isolated from …
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Purification and characterization of an endo-1,4-{u03B2}-D-glucanase and two exo-1,4-{u03B2}-D-glucanases from the cellulase system of Trichoderma reesei
… amino acids, but much less of basic amino acids. Sedimentation equilibrium studies yielded the following molecular weights for the glucanases: cellobiohydrolase I (D) 53,220±1479; cellobiohydrolase II 54682±2683; endoglucanase 45,215±1483. Cellobiohydrolase I (D) is the most acidic (pH<sub>I</sub> …
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Solution Conformations and Structural Thermodynamics of Type I DNA Polymerases from Escherichia coli and Thermus aquaticus
The global conformations of DNA polymerase I from Thermus aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then …
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Development of MWL-AUC / CCD-C-AUC / SLS-AUC detectors for the analytical ultracentrifuge
… physicochemical parameters. For example, sedimentation equilibrium experiments can lead to the study of pure thermodynamics. For complex mixtures, AUC is the main method that can analyze the system. Interactions between molecules can be studied at different concentrations without …