Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"secretory vesicles"”.
-
Molecular mechanisms of snare assembly and expulsion of intravesicular contents in cell secretion
… that during cell secretion, membrane-bound secretory vesicles completely merge at the cell plasma membrane resulting in the diffusion of intra-vesicular contents to the cell exterior and the compensatory retrieval of the excess membrane by endocytosis. This explanation made no sense or …
-
Immunocytochemical light microscopy and transmission electron microscopy study of growth hormone secreting cells in the porcine anterior pituitary
… gland store GH in the 350-500 nm in diameter secretory vesicles that may dock and transiently fuse at the plasma membrane fusion pores [POROSOME] to secrete GH. The earlier AFM study demonstrated 40% increase in size of the fusion pore after stimulation of GH secretion. The objectives of our …
-
Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
… exocytosis. We investigate PLD1 localization to secretory exocytic machinery and cargo; using a pH-sensitive probe for secretory vesicles in PC12 cells, we observe this localization to sites of exocytosis. PLD1 is present on secretory vesicles throughout the process from trafficking, to docking …
-
MUTATION OF THE VESICULAR MONOAMINE TRANSPORTER-1 GENE ALTERS ITS PROTEIN PRODUCT
… such as epinephrine and serotonin, in secretory vesicles of neuroendocrine cells. Recently the VMAT1 protein was detected in human and mouse brain, and mutations of the VMAT1 gene at single DNA nucleotides (single nucleotide polymorphisms or SNPs) were associated with schizophrenia. In …
-
Altered Morphology And Composition Of Zymogen Granules In Acute Pancreatitis
… as inactive zymogens within membrane bound secretory vesicles called Zymogen Granules (ZG), and activated extracellularly. In acute pancreatitis however, the digestive enzymes are prematurely activated within the cell, resulting in autodigestion of the tissue. Pancreatitis is a …
-
Cell Polarity Establishment in the Budding Yeast Saccharomyces Cerevisiae
… acts as tracks to facilitate the delivery of the secretory vesicles that will grow the bud, an essential process for an organism that proliferates by budding. We have employed treatment with the actin depolymerizing drugs Latrunculin A and B as well as high-speed timelapse microscopy of …
-
Biochemical analyses of the Golgi and its associated membrane proteins in Saccharomyces cerevisiae
Secretory trafficking in eukaryotic cells occurs via membrane-enclosed organelles and vesicular intermediates which deliver proteins and lipids to their cellular destinations. Throughout this process, the unique nature of organelles is likely to be maintained through: (i) membrane recycling events …
-
Mechanisms of Cell Surface Trafficking and Potential Functions of Extracellular Annexin A2
… extracellular secretion by association with the secretory vesicles called exosomes. In our studies, we also investigated the role of AnxA2 in inducing neovascular responses. We have shown that angiogenic insults like hypoxia upregulate the expression of AnxA2. AnxA2-induced angiogenic responses …
-
The SEC9 gene is essential for exocytosis in yeast and encodes a dispensable amino terminal domain
… SEC9 gene product is necessary for transport of secretory vesicles from the Golgi complex to the plasma membrane. The SEC9 gene was cloned from a yeast genomic library by complementation of the temperature sensitive growth defect of sec9-4 cells. The SEC9 gene is essential for viability in yeast …
-
The SAC1p, an integral membrane protein involved in secretory pathway function and actin function in Saccharomyces cerevisiae
The secretory pathway of the yeast Saccharomyces cerevisiae is strictly analogous to that of mammalian cells. Proteins destined for secretion are transported in a vectorial fashion from the endoplasmic reticulum to the Golgi apparatus to the cell surface. Superimposed on the normal flow of …
-
Lipid rafts in protein sorting and yeast cell polarity
… sought to characterize the lipid composition of secretory vesicles. Hence, we established a vesicle purification protocol based on an immunoisolation strategy. Additionally, in collaboration with the group of A. Shevchenko, we developed a mass spectrometry methodology that allows the …
-
Novel regulators of trafficking in the yeast Golgi-endosomal system
… a key event in this process, the formation of secretory vesicles at the Golgi and sorting of cargo into these transport carriers, remains poorly understood. It has been demonstrated that phosphatidylinositol 4-phosphate (PI(4)P) generated by the PI(4)-kinase Pik1p plays an essential role in …
-
Characterization of the Human Adenovirus 19a E3/49K Protein
… proteolytic processing may occur in the TGN or secretory vesicles and/or at the cell surface. The finding that an increased cell surface expression of 49K correlates with an increased secretion rate indicates proteolytic processing of 49K at the cell surface. The C-terminal fragments and also …
-
Characterisation of L-cell secretory mechanisms and colonic enteroendocrine cell subpopulations
… prevalent co-storage of GLP-1 and INSL5 in secretory vesicles and similar secretory responses of both hormones to a range of different stimuli strengths (including short chain fatty acids, angiotensin II and arginine vasopressin (AVP)) it was concluded that GLP-1 and INSL5 are co-secreted, …
-
Identification and characterisation of the Spred protein family
… level its immunoreactivity was associated with secretory vesicles and was found to colocalise with Rab11 GTPase. The new human Spred gene family was investigated in detail. Cloning of the fulllength form of human Spred-2 resulted in an 1254 bp coding sequence, corresponding to a 418 amino-acids …
-
Cell Biology of the ICA69 protein family in Neurosecretory cells
… proteins associated with Golgi and post-Golgi secretory vesicles, including insulin secretory granules and synaptic-like microvesicles. Thus, the study of beta-cell autoantigens with yet unknown function may provide novel insight into the secretory machinery of beta-cells and led to the …