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Showing 1 to 20 of 160 for “"secretion system"”.
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Helicobacter pylori Type 4 Secretion System Modulates Host T Cell Responses
… demonstrated that H. pylori requires its type 4 secretion system (T4SS) translocated components: effector protein CagA and cell-wall peptidoglycan (PG) fragments for up-regulating B7-H1 as well as B7-H3, and for down-regulating B7-H2 on GEC. These data were validated in vivo by using a mouse …
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Structural characterization of the type II secretion system of Aeromonas hydrophila
The exeC gene, found in the gram-negative bacteria Aeromonas hydrophila codes for a 31 kDa, three domain, bitopic inner membrane protein. The components of the ExeC protein include an amino-terminal cytoplasmic domain, a trans-membrane helix and two periplasmic domains. The two periplasmic domains …
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Regulation of the Salmonella Pathogenicity Island 1 Type Three Secretion System
… serovar Typhimurium is mediated by a Type Three Secretion System (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The SPI1 TTSS injects effector proteins into the cytosol of host cells where they promote actin rearrangement and engulfment of the bacteria. HilC, HilD, and RtsA activate …
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Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… have been described, so far in E. coli to allow secretion of proteins: The Sec, the Tat and the SRP (Signal Recognition Particle) pathway. While the Sec and the Tat pathway act post-translationally and accept unfolded and correctly folded polypeptides, respectively, the SRP pathway acts …
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Characterisation of the Type VII secretion system of <i>Staphylococcus aureus</i>
The Type VII secretion system (T7SS), is a protein secretion pathway found in Gram positive bacteria. In S. aureus the T7SS, termed Ess, is dispensable for laboratory growth but is essential for virulence. The Ess system is encoded by the ess locus, the first six genes of which esxA, esaA, esaB, …
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A Bioinformatics Analysis of Bacterial Type-III Secretion System Genes and Proteins
Type-III secretion systems (T3SSs) are responsible for the biosynthesis of flagella, and the interaction of many animal and plant pathogens with eukaryotic cells. T3SSs consist of multiple proteins which assemble to form an apparatus capable of exporting proteins through both membranes of …
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Investigating the key components of the chitinase secretion system in <i>Serratia marcescens</i>
… of the extracellular polysaccharide chitin. Secretion of these proteins appears to be a two-step process in <i>S. marcescens</i> with initial export to the bacterial periplasm being followed by a final secretion step across the outer membrane. Successful secretion of the chitinolytic …
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Characterisation of the putative Type VII protein secretion system of <em>Streptomyces</em> spp
… encoding components of putative Type VII protein secretion systems (T7SSs). To investigate the role of these putative T7SSs in S. coelicolor and S. scabies, marked deletions were constructed in genes encoding core components of the machinery. Phenotypic analysis revealed that deletion of genes …
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Structural study of ExsA, the regulator of Type III Secretion System of Pseudomonas aeruginosa
The Type III secretion system (T3SS) of Pseudomonas aeruginosa uses a needle-like protein apparatus to detect eukaryotic host cells and translocate effectors directly into the host cell. The effectors are also known as cytotoxins, which cause disruption of a series of signaling events in the host …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
… <em>tumefaciens</em> VirB/D4 type IV secretion system (T4SS) delivers oncogenic T-DNA and effector proteins to susceptible plant cells. This leads to the formation of tumors termed Crown Galls. The VirB/D4 T4SS is comprised of 12 subunits (VirB1 to VirB11 and VirD4), which assemble to …
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Tagging of the type three secretion system basal apparatus of Enterohaemorrhagic Escherichia coli 0157:H7
… coli (EHEC) O157:H7 possesses a type III secretion system (T3SS). The T3SS translocates effector proteins into host cells during infection and consists of a basal apparatus that spans the inner and outer membranes and a needle and filament complex. The basal apparatus is composed of -20 …
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Characterizing the Role of the Type VI Secretion System in Interbacterial Species Interactions and Pathogenesis
… bacteria have adapted is the use of type VI secretion systems (T6SS). The T6SS is a large needle complex that spans across the entire bacterial cell wall and functions through a contraction mechanism that results in the delivery of toxic effector proteins into neighbouring cells. Virulence is …
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Exoproteomics of the Pseudomonas aeruginosa biofilm matrix reveals the role of a novel secretion system
… of cells living in a matrix of their own secretions. Cells that would otherwise be susceptible to antimicrobials are protected by the modifications that they make, in concert, to their surroundings. Aggregates of as few as 64 cells begin to exhibit elevated tolerance to antibiotics and, …
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Molecular Interactions of Type III Secretion System Transcriptional Regulators in Pseudomonas aeruginosa: ExsA and ExsD
… causes of nosocomial infections. The type III secretion system (T3SS) aids acute P. aeruginosa infections by injecting potent cytotoxins (effectors) into host cells to suppress the host's innate immune response. Expression of all T3SS-related genes is strictly dependent upon the transcription …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
… gram-negative pathogen utilizes the Type Three Secretion System to transport effector proteins into the host cell. Several proteins are essential for the transportation of these effectors, they are the secretion needle subunit, AscF and pore forming translocators, AopB and AopD. Prior to …
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Characterisation of YGT a novel Type III secretion system in Biotype 1A Yersinia enterocolitica strain YE53/03
… some BT 1A strains. Recently, a novel Type III secretion system (TTSS) has been discovered named YGT; this was present in the chromosome of Y. enterocolitica non-pathogenic BT, but it is not yet characterised. Considering that TTSSs are essential for the pathogenicity of many bacteria, this …
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The Chlamydia Trachomatis Protein Interaction Network: Insights into the Unique Composition of the Type Three Secretion System
… of genetic tools, we developed a yeast model system to identify and characterize chlamydial proteins involved in virulence mechanisms. In this study we describe the identification of twenty-eight proteins potentially involved in modulating host cellular functions and the secretion of virulence …
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A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
… 3 decades, the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several entities: unfolded precursor polypeptides, the molecular chaperone SecB, membrane …
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