Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 82 for “"secreted proteins"”.
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Engineering Secreted Proteins for Gene Transfer and DNA Vaccination
… to address some aspects of these issues.</p> <p>Secreted reporter proteins are promising tools to study gene transfer and expression in a non-destructive manner, and bioluminescent proteins are particularly convenient to use for that purpose. To generate a secreted bioluminescent marker protein, …
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Giardia trophozoite-secreted proteins and their effects on intestinal epithelia
… enteric cells, consistent with the production of secreted virulence factors by the parasite. Quantitative proteomic analysis was successfully applied to the whole parasite and supernatants derived from the parasite in order to ascertain which parasite proteins are secreted. The genome of Giardia …
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Investigating the inhibitory effects of secreted proteins on macrophage in glioblastoma
… and inhibition of T cell activity through secreted immuno-suppressive proteins and cytokines, dysfunction of immune cells and upregulation of immune checkpoint molecules. Macrophages are the most populous non neoplastic cells in glioblastoma microenvironment, which is a part of the innate …
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A FAMILY OF SMALL SECRETED PROTEINS FROM RHIZOPHAGUS IRREGULARIS CONTRIBUTE TO ARBUSCULAR MYCORRHIZA SYMBIOSIS
… genetically modify the fungus. To date, only two secreted proteins from Rhizophagus irregularis have been described and known to participate in the symbiosis. However, based on recent transcriptomic data, more secreted proteins have arisen as potential participating candidates in development of …
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The effects of the adipocyte-secreted proteins resistin and visfatin on the pancreatic beta-cell
Adipose tissue secreted proteins (adipokines) have been proposed to form a link between obesity and type 2 diabetes (T2D). Resistin and visfatin are two adipokines which have been previously suggested as having roles in the pancreatic islet. The aim of this study was therefore to investigate the …
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Proteomic Characterization of Human Multipotent Stromal Cells Secreted Proteins with Therapeutic Potential for β-cell Regeneration
… these methods to identify important factors secreted by hMSC for β-cell regeneration, as well as functionally investigating candidate proteins and refining current models of hMSC mediated β-cell regeneration. In working towards these goals, we first assessed the advantages and disadvantages …
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Tracing of Alpha-to-Beta Cell Transition Following Treatment with CHIR-Injected Human Multipotent Stromal Cell Secreted Proteins
Diabetes is a global epidemic projected to affect over 628.6 million people by 2045. Current insulin therapy manages symptoms but cannot restore endogenous beta cell function. Regenerative strategies have demonstrated beta-cell neogenesis following ablation; however, the cellular mechanisms driving …
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Peptides as amino acid sources for the synthesis of secreted proteins by mammary tissue explants and cultured mammary epithelial cells
… lysine sources respectively for the synthesis of secreted proteins. Mammary tissue explants from lactating (10 to 11 d) CD-1 mice and cultured bovine mammary epithelial cells (MAC-T) were used as experimental models. Explants and cultured cells were incubated at 37°C in a humidified atmosphere of …
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Mycobacterium Tuberculosis Virulence Factor Mpt64 Targets the Endoplasmic Reticulum
… niche. I hypothesized that M. tuberculosis secreted proteins that target host membranes are vital for M. tuberculosis to adapt to and manipulate the host environment for survival. Thus, I characterized nearly 200 secreted proteins from M. tuberculosis for their ability to associate with …
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Mass spectrometry based proteomic strategies applied in the study of central nervous system derived cells
… for identification and quantitation of proteins. Development of such strategies is important to achieve a better understanding of regulatory mechanisms of individual cell types, both during normal development and during the onset and progression of diseases. Cells derived from the …
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Characterization of protein secretion in Mycobacterium leprae using phoA fusions in Escherichia coli and Mycobacterium smegmatis
… genome has provided new information related to proteins constituting its hypothetical proteome. Since M. leprae can not be grown in vitro, novel approaches are needed to determine which proteins are expressed during infection and whether these proteins are related to pathogenesis. Secreted …
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Adiporedoxin, an upstream modulator of endoplasmic reticulum oxidative folding and protein secretion
… extracellular matrix, and transmembrane proteins and over expression increased secretion. Together, these results suggest Adrx regulates an early step in protein secretion from the ER. Immunofluorescence and proteolytic protection assays demonstrated that Adrx is located in the ER …
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The role of BIP and PDI in the secretion of foreign proteins in the yeast Saccharomyces cerevisiae
… such as post-translational modifications of secreted proteins. One disadvantage, however, is that secretion of foreign proteins is generally inefficient.
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Oxidative Protein Folding Pathways In Gram-Positive Actinobacteria
… bonds are important for the stability of many secreted proteins. These covalent linkages, which result from the oxidation of neighboring cysteine (Cys) residues, are often rate-limiting steps for protein folding and maturation. Disulfide bond formation is restricted to extracellular oxidizing …
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Evaluating The Impact of Post-Translational Modifications By The Secreted Zinc Metalloprotease, Gele, On The Major Autolysin of E. Faecalis, Atla, and A Stress-Induced Protein, Salb
… in cell separation of dividing cells. SalB is a secreted stress-induced protein regulated by the CroRS system. In addition, these two proteins also appear to be affected by the virulence factor, gelatinase (GelE). GelE is a secreted zinc metalloprotease known to impact various cellular functions …
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Early Events in the Biosynthesis and Subcellular Consignment of Secretory and Membrane Proteins
… mechanisms responsible for the segregation of secreted proteins and for the insertion of integral membrane proteins have been examined in light of the signal hypothesis. Model systems for the study of the biosynthesis of these different classes of proteins, as well as for the study of specific …
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Pathogen-induced protein secretion in plants.
… and absolute quantification. A total of 76 secreted proteins were identified, 66 of which showed differential secretion patterns in response to SA. A majority of induced protein secretion was observed within the first two hours after treatment, suggesting many proteins are involved in the …
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The Role of Oxygen and Oxygen-Dependent Enzymes in Protein Folding, Metabolism, and Redox Homeostasis
… enzyme, 2-aminoethanethiol dioxygenase (ADO). Secreted proteins contribute to aggressive cancer phenotypes in the TME. These secreted proteins are thought to utilize an oxygen dependent mechanism for disulfide bond formation required for proper folding, which represents a paradox when they are …
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Investigating the Role of Trimeric Autotransporter Adhesins in Fusobacterium nucleatum Pathogenesis
… available to functionally analyze F. nucleatum proteins. Interestingly, F. nucleatum has a diverse landscape of structurally unique surface adhesins called Type 5c secreted trimeric autotransporter adhesins (TAAs), which are a family of proteins that are historically known for their …
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