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Showing 1 to 5 of 5 for “"sec14"”.
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Characterization of SEC14: A gene essential for secretion in Saccharomyces cerevisiae
SEC14, a Saccharomyces cerevisiae gene whose product is required for export of yeast secretory proteins from the Golgi complex, was cloned and characterized. SEC14 was shown to be an essential gene for vegetative growth. Nucleotide sequencing revealed SEC14 to be an intron-containing gene, which …
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
… with assistance from the PI/PC transfer protein Sec14 (4). It is proposed that Sec14 stimulates the activity of Pik1 by partially removing PI from the bilayer and presenting it to the kinase as a more suitable substrate (4). In order to test this hypothesis, the Bellbrook Labs Transcreener ADP2 …
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Golgi phospholipid composition is a critical determinant of Golgi secretory competence in yeast
… is strictly dependent upon the activity of the SEC14 gene product, the Sec14p. Yeast strains bearing temperature-sensitive mutations in the SEC14 gene exhibit defects in protein trafficking from the Golgi and otherwise wild type cells which lack any functional copy of the sec14 gene fail to …
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Synthesis and Evaluation of Trans-bilayer Cross Linking Phospholipids Based on Click (Azide-Alkyne) Chemistry
This thesis describes the synthesis of two phosphatidylcholine (PC) derivatives that are capable of coupling with each other from the alkyl chain through click reaction to form bola-PC. Successful coupling of monomeric phosphatidylcholine derivatives would lead to synthesis of corresponding …
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THE OPTIMIZATION OF A TIME-RESOLVED, VESICLE-BASED FLUORESCENCE ASSAY FOR THE ACTIVITY OF THE LIPID KINASE PI4K-IIIBETA AND THE EFFECT OF LIPID TRANSFER PROTEINS ON ENZYME ACTIVITY
… PI transfer proteins (PITPs). In yeast, the PITP Sec14p is known to exchange PI and phosphatidylcholine between lipid bilayers in vitro and proposed to present PI to be phosphorylated by the PI4-kinase Pik1 in a heterotypic ligand exchange fashion. However, the precise mechanism by which this …