Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"sdAb"”.
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Molecular studies of the sdaA and sdaB genes and their gene products in Escherichia coli K-12
… expression in minimal medium. An insertion in sdaB abolished L-SD#2 activity in an sdaA::Cm$\sp{\rm r}$ sdaX strain, allowing the sdaB gene to be cloned by restoring growth on L-serine. The sdaA gene was located at 41 minutes; sdaB and sdaX both were located near 60.1 minutes and may be the …
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting Clostridium difficile enterotoxin A
Single domain antibodies (sdAb) have emerged as a family of novel antibodies that have vast applications. Of particular relevance, ToxA5.l, a sdAb capable of binding to, and therefore neutralizing, Clostridium difficile toxin A (TcdA) has been developed. The antibiotic resistant version of C. …
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Chemical Mutagenesis for the Modulation and Study of Protein Function and Dysfunction within Alzheimer’s Disease
… relationship study on a single-domain antibody (SdAb) beyond the 20 canonical AAs. The starting SdAb was designed to target the Amyloid-b peptide (Ab) and has a nascent ability to inhibit its aggregation, a key pathological process in AD. Utilizing the synthetically versatile non-canonical AA …
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Rational engineering strategies to improve antibody fragment pharmacokinetics and bioavailability
… fragments, including single-domain antibodies (sdAb), as therapeutics, as their small size affords favorable properties such as increased tumor penetration as well as the ability to utilize lower-cost prokaryotic production methods. However, they suffer from poor pharmacokinetics in vivo. This …
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Cross-reactivity among alphaviruses provides insight into viral emergence and novel defense strategies
… of a camelid-derived single-domain antibody (sdAb) that neutralizes a breadth of alphaviruses, including CHIKV and MAYV. With these data, we have designed and generated transgenic Aedes aegypti mosquitoes that express two anti-CHIKV sdAbs to target infection, dissemination, and transmission of …
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Kinetics of Disordered Proteins and their Interactions
… specially engineered single-domain antibodies (sdAbs) with a modified complementarity determining region (CDR). These complementarity determining regions (CDRs) are often disordered and their dynamics are poorly understood. I performed enhanced sampling simulations of both an sdAb designed using …
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Systematic development of rationally designed antibodies targeting predetermined epitopes of interest and protein aggregation
… regions (CDRs) of single-domain antibodies (sdAbs) towards the target regions. This was shown to bind disordered epitopes specifically and with good affinity. The binding was also shown to inhibit the aggregation of αSyn. This approach was also utilized to create multiple sdAbs binding …
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Isolation and Characterization of Anti-SLP Single Domain Antibodies for the Therapy of C. difficile Infection
… recombinant single-domain antibodies (sdAbs), in terms of affinity and specificity, intrinsic stability, and ability to inhibit cell motility. Several sdAbs exhibit promising characteristics for a potential oral therapeutic based on their high affinity, high thermal stability, and …