Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"screening assay"”.
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Identifying Genes in Pancreatic Beta-Cell Function using a Novel High-Thoughput Cell-Based Screening Assay
… Therefore, a highthroughput surrogate cell-based assay for pancreatic beta-cell insulin secretion has been developed through which the screen of novel genes and the genes having genetic evidences of susceptibility to type 2 diabetes were screened, in order to assign them pertinent roles in …
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Development of a new screening assay to identify proteratogenic compounds using Zebrafish Danio rerio embryo combined with an exogenous mammalian metabolic activation system (mDarT)
… generally performed using in vivo teratogenicity assays e.g., in rats or rabbits. Following the 3R principles, the development of alternative methods is encouraged to reduce the number of animal tests. From this perspective, we have developed an in vitro assay (mDarT) using the zebrafish Danio …
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IDENTIFICATION OF CDC7 AS A NEW REGULATOR OF T CELL ACTIVATION
In this thesis, a novel screening assay was designed to identify novel mediators of TCR signalling. A library of kinase inhibitors was screened for their effects on suppressing activation in several T cell populations. The screening assay was able to identify inhibitors of canonical kinases of TCR …
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Advanced systems for the rapid detection of anthelmintic drugs in food
Several surface plasmon resonance (SPR) biosensor assays were developed and validated for the detection of anthelmintic veterinary drugs in liver tissue and milk using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction procedure. The first screening assay was developed to detect …
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Engineering of an efficient and enantioselective biocatalyst for the preparation of chiral pharmaceutical intermediates
… two-enzyme-based colorimetric high-throughput ee screening assay and a mass spectrometry-based high-throughput ee screening assay were developed. The P450pyr monooxygenase was engineered by directed evolution for the enantioselective hydroxylation of N-benzyl pyrrolidine. Several mutants …
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Biophysical and Biochemical Screening Approaches for Antimicrobial Drug Discovery Targeting S. aureus ClpP
… of conventional antibiotics drug discovery screening approaches, and transitions to modern era structure or fragment based screening approaches. The merits and challenges of such approaches of targeting a well conserved bacterial protease (ClpP) are discussed along with dissertation aims …
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Development of chemical inducers of dimerization for screening competitive histone deactelyase inhibitors
… including those not involved in cancer. To date, screening for HDAC inhibitors has been done using an in vitro fluorescence-screening assay, which is unable to test for selectivity. Alternatively, HDAC inhibitors are screened against commercially produced proteins, which is very time consuming and …
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Novel Modulators of Human Sperm Motility
… for motility, a Flexstation high-throughput screening assay (HTS) of 3312 drug discovery ion channel compounds was performed. The screen identified 14 hit compounds that increase intracellular calcium in human spermatozoa. This project investigated these compounds, along with, 6 hit compounds …
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South African marine compounds as anticancer agents
… this thesis we describe the establishment of a screening assay using an oesophageal cancer cell line as a model. It was our hope that this screen would allow us to identify compounds which have activity against oesophageal cancer, that could be used as lead agents for further development of …
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SERS-active peptide nanoparticles for investigating protein allostery and bionanoassembly
… with p53, thus providing an allosteric assay for identifying novel binding ligands. SERS (Surface enhanced Raman scattering) - active nanoparticles, functionalized with a p53 peptide mimic, peptide 12.1, display biologically specific aggregation following addition of MDM2. The extent of …
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… of a code with a single stage high throughput screening assay. The work outlined here describes the development of a model system for the study of DNA protein interactions and the development of a high throughput assay for detection of such interactions. A zinc finger protein was designed which …
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… very versatile tools that have been applied in assay development for various purposes. They have been made into genetically encoded biosensors/probes or affinity agents for biomarker detection. This thesis explored a few topics using assays developed with engineered proteins. The genetically …
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Identification, Validation, and Mapping of Phytophthora sojae and Soybean Mosaic Virus Resistance Genes in Soybean
… The majority of this research is focused on screening for novel sources of P. sojae resistance with core effectors to identify resistance genes (R-genes) that will be durable under field conditions. Four segregating populations and two recombinant inbred line (RIL) populations have been …
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Biochemical Analysis of Evolutionary Divergent Vertebrate Larp6 Proteins
… coli with an N-terminal SUMO tag. A filtration screening assay was used to identify additives that increase solubility and stability to the fish proteins. To robustly compare direct RNA binding affinity of the fish proteins to the human LARP6, the SUMO tag was cleaved by Ulp1. Limited …
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Development and assessment of new sensor systems in food packaging applications
… assurance of food safety systems. This automated screening assay produced characteristic profiles with a sharp increase in fluorescence above the baseline level at a certain threshold time (TT) which can be correlated with their initial microbial load and was applied to various raw fish and …
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Noninvasive disease diagnostics using engineered synthetic urinary biomarkers
… with clinical ELISA and paper-based lateral flow assays. These detection techniques enable simple and inexpensive quantification of our synthetic disease reporters by ensuring compatibility with existing diagnostic resources and infrastructure. To demonstrate our platform's versatility, we adapted …
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The Translationally Controlled Tumor Protein (TCTP) associates to and destabilizes the Circadian Factor Period 2 (Per2)
… stability. In this work, we perform a two-hybrid screening assay using the C-terminal region of human Per2 and identified an extensive number of interactors. Utilizing a genetic ontology program, we assorted the list of clones into groups of proteins that are biologically relevant or operated in …
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New Approaches to the Development of Peptoid Vaccines
… a vaccine platform, it was hypothesized that screening large one-bead-one-compound libraries of synthetic compounds with monoclonal antibodies that have already been shown to be broadly protective against a toxin or pathogen would allow the identification of mimetic B cell epitopes. For this …
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Development and Application of a Novel Assay for Rapid Identification of Cellular Prion Protein Modulators
… application of an innovative BiFC-based in vitro screening assay, Light-Identification of Protein Suppressors (LIPS), designed for real-time monitoring of PrPC expression and modulation. The LIPS system is based on the Bimolecular Fluorescence Complementation (BiFC) technique, usually used for …
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Maize Transformation for Resistance to Aspergillus Flavus
… Northern and Western blots, and enzyme activity assays. The ch18 transgene was expressed in embryos as expected and the embryos also had higher chitinase activity than that of the untransformed controls beginning 17 DAP. However, higher beta-1,3-glucanase activity was not detected although the …
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