Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"sclerostin"”.
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The effects of stroke on the skeleton
… in cancellous, endocortical and cortical bone. Sclerostin, a newly discovered osteocyte-derived protein was studied using immunohistochemical staining of the bone biopsies. Sclerostin is known to be an inhibitor of active osteoblasts, which led to the hypothesis that in stroke, the proportion of …
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The role of osteocytes in disuse and microgravity-induces bone loss
… first evidence of a cell-autonomous increase in sclerostin, a potent inhibitor of Wntsignaling, following exposure to simulated microgravity. These cells were also used for a spaceflight mission after demonstrating their ability to maintain an osteocytic phenotype when cultured in a fully …
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Bone health in elite ballet dancers: a multidisciplinary approach
… I (IGF-1), oestrogens, growth hormone, and sclerostin serum concentrations were longitudinally measured in a sub-sample of 101 vocational dance students and age- and sex-matched controls. Association between polymorphisms of the Wnt/β-catenin and ER signalling pathways with low BMD were …
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Neural and Physical Niches Orchestrate Skeletal Remodelling and Promote Cartilage Regeneration
… to increased expression of the Wnt inhibitor, sclerostin, in osteocytes, and inhibition of sclerostin in Gfra2<sup>-/-</sup> mice rescued deficits in bone formation. Overall, skeletal cholinergic innervation provides neurotrophic signals and suppresses sclerostin in osteocytes, promoting bone …
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The Effects of a Single Bout of Plyometric Exercise on Anabolic and Catabolic Osteokines in Girls and Adolescents
… related to the anabolic Wnt signalling pathway [sclerostin and dickkopf 1 (DKK-1)] and the catabolic RANKL pathway, [osteoprotogerin (OPG), receptor activator of nuclear factor kappa-β ligand (RANKL)], as well as the related transforming growth factors (TGF-β1, TGF-β2 and TGF-β3) to an acute bout …
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Mechanical Properties of Bone Due to SOST Expression: A 3-Point Bending Assessment of Murine Femurs
<p>Sclerostin, a protein coded for by the SOST gene, is an osteocyte-expressed negative regulator of bone formation. The absence of SOST in the genome may have an effect on bone formation both during skeletal maturation and full maturity. This study attempts to determine significant differences in …
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The Role of Wnt Signaling in Bone Mechanotransduction
… I hypothesize that the mutation compromises sclerostin action on bone cells. Understanding how each of these components of the Wnt signaling pathway interact, may lead to novel therapeutic targets for treatment of bone diseases.
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Physical training, inflammation and bone integrity in elite female rowers
… properties and factors that regulate bone (sclerostin (SOST), osteoprotegerin (OPG)), and receptor activator of nuclear factor kappa-B ligand (RANKL)) and energy metabolism (insulin-like growth factor-1 (IGF-1) and leptin), and inflammation (tumor necrosis factor-α (TNF-α)) in elite …
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Hormonal regulation of bone health: novel understandings of xenoestrogens and estrogen receptor-[alpha]
… that ERKO could lead to increased expression of sclerostin, a bone growth inhibitor, in aged, male mice. In younger, male ERKO mice we found that ERa is not required for an osteogenic response to exercise, which is in direct contrast with females. Finally, we found that gestational and …
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Interactions Between Aging and Chronic Kidney Disease on the Skeleton
… pores. Romosozumab, a humanized monoclonal sclerostin antibody, uses PTH-independent mechanisms to increase osteoblast activity, making it an attractive therapeutic for CKD. CKD was induced by feeding aging (78-week) male mice 0.2% adenine for six weeks followed by two weeks of maintenance …
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Multifaceted computational modelling in pharmaceutical research and development
… a small database of phytopharmaceuticals against sclerostin protein followed by an investigation of the stability of the protein-ligand complexes through standard molecular dynamics and funnel meta dynamics. The fourth chapter involves the modelling of adsorption and diffusion of doxorubicin, …
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Osteocyte Activity and Skeletal Muscle Relative Gene Expression Profiling After Short-term Muscle Paralysis
… One-week post-injection, osteocyte expression of sclerostin (Scl), osteocyte apoptosis (caspase-3), perilacunar remodelling (cathepsin K), and staining of vascular pores and mast cells were assessed using immunohistochemistry. Real-time polymerase chain reaction (qPCR) was performed on the …
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SOSTDC1: A BMP/Wnt Dual Antagonist in Breast and Renal Cancers
… of transformation in normal cells. SOSTDC1 (SclerOSTin Domain-Containing-1) is an inhibitor of BMP/Wnt signaling in mouse disease models; however, little is known about the expression or actions of SOSTDC1 in human tissue. This work expands upon the observation that SOSTDC1 downregulation …
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Automatic GUI Based Counting Software for Immunostaining Analysis Using Matlab GUIDE
… positive cells are good, but moderate in case of Sclerostin. This automated GUI software method is a major advancement in terms of osteocyte counting from histological sections, and the procedure is explicitly compatible to diverse staining methods and color combinations. A standalone application …
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Exploring Molecular Mechanisms Controlling Skin Homeostasis and Hair Growth. MicroRNAs in Hair-cycle-Dependent Gene Regulation, Hair Growth and Associated Tissue Remodelling.
… components of Wnt and BMP signalling pathways Sclerostin and BAMBI, and Dlx3 transcription factor, as well as selected keratin genes. Luciferase reporter assay revealed that Krt16, Krt17, Dlx3, and Fgf10 serve as direct miR-31 targets. In addition, miR-214 was identified as a potent inhibitor …
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Interactions between BMP and Canonical Wnt Signaling Regulate Critical Stages of the Osteoblast Lifecycle
… of the canonical Wnt pathway, such as Sclerostin and Dickkhopf1, are currently in clinical trials as potentially promising bone anabolic agents. However, it is still unclear how Wnt signaling provides such a strong osteogenic stimulus and how it interacts with BMP signaling, another …
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Assessment and Dynamic Monitoring of Hyperphosphataemia. Predictor for Bone-Mineral Disorders in Dialysis Patients // Оценка и динамично проследяване на хиперфосфатемията – предиктор за костно-минерални нарушения при диализни пациенти
Дисертационният труд има за цел да се анализират диагностичните, клинични и терапевтични аспекти на нарушенията на костно-минералния метаболизъм при хроничните бъбречни заболявания в хода на консервативното и хемодиализно лечение. Да се анализират нови неинвазивни биомаркери (Склеростин) за оценка …