Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 64 for “"scaffold protein"”.
-
The scaffold protein POSH regulates T lymphocyte function
… to the TCR. This dissertation shows that the scaffold protein Plenty of SH3s (POSH) is required for optimal JNK activation and effector function in both CD4+ and CD8+ T cells. Additionally, this work shows that POSH is dispensable for JNK activation and positive and negative selection in …
-
Investigation of The Role of The Scaffold Protein Shc In Erk Signaling
… extracellular signals into intracellular events. Scaffolding proteins play important roles in generating specificity in intracellular signaling through the combinatorial use of protein domains and posttranslational modifications. Using the scaffold Shc (Src-homology collagen-like) as a model …
-
A role for the SHANK1 scaffold protein in experience-induced synaptic plasticity and memory consolidation
… synaptic development and plasticity. SHANK1 is a scaffolding protein that is concentrated to the postsynaptic density (PSD) of excitatory synapses and is involved in the binding of glutamate receptors to their active zones. Previous developmental studies have demonstrated that SHANK1 is initially …
-
Tissue specific regulation of the high density lipoprotein (HDL) receptor, scavenger receptor Class B, Type I (SR-BI) by the scaffold protein PDZK1
… a four PDZ-domain containing cytoplasmic adaptor protein that binds the Cterminus of the high-density lipoprotein (HDL) receptor SR-BI. Abolishing PDZK1 expression in PDZK1 knockout (KO) mice leads to a post-transcriptional, tissue-specific decrease in SR-BI protein level and an increase in total …
-
Multidimensional Solid-State NMR Studies of Membrane Proteins
… to study the structure and dynamics of membrane proteins by using uniformly or extensively isotopically labeled samples. In this work, we first optimized the performance of shaped pulses in magic-angle spinning solid-state NMR experiments by characterizing their frequency profiles and refocusing …
-
Metal Thiolate and Metal Sufide Clusters in Proteins
… histidine coordination to the copper atoms. A scaffold protein was selected, and residues were chosen for mutagenesis to create a similar ligand set to CuZ in N2OR. The copper binding properties and copper/sulfide binding properties of the biosynthetic and native N2OR systems were investigated …
-
Intracellular and extracellular promoters of metastasis to different organs
… aspects of metastasis. First, I investigated the scaffold protein IQGAP1, which promotes primary tumor growth and invasiveness in several cancers. However, the role of IQGAP1 in metastasis has been unclear. We used IQGAP1 knockdown and knockout to investigate its role in the metastatic cascade in …
-
Atg11 and Atg9 interaction in selective autophagy
… organizer of selective autophagy, acts as a scaffold protein, interacting with Atg9, Atg1 and other Atg proteins. Our research focuses on finding mutations in Atg11 that specifically disrupt its interaction with Atg9 without affecting its dimerization or interaction with Atg1. We targeted …
-
Mosaic Analysis of Neurogenesis and Neuronal Morphogenesis in Drosophila Learning and Memory Center Mushroom Bodies
… two genes are Cul3 and Chinmo. Cul3 encodes a scaffold protein for E3 ubiquitin ligase. Phenotypic analysis demonstrates that Cul3 is required for proper axon arborization and dendritic elaboration of MB neurons. Chinmo is a novel BTB-zinc finger protein. Functional analysis shows that Chinmo …
-
Structural and Molecular Mechanisms of Centrosome Assembly and Strength
… multimerization of SPD-5, the main PCM scaffold protein in C. elegans. In the unassembled state, SPD-5 exhibits numerous intramolecular interactions that are eliminated after phosphorylation by PLK-1. Thus, phosphorylation induces a structural opening of SPD-5 that primes it for …
-
Characterization of Tuba, a novel Ena/VASP ligand, and function of Ena/VASP proteins in mouse development
… adhesion and shape change. The EnaNASP family of proteins controls actin filament elongation and are important regulators of axon guidance and cell motility. In vertebrates, the family consists of Mena (Mammalian Enabled), VASP (Vasodilator Stimulated Phosphoprotein), and EVL (Ena-VASP-Like). This …
-
Creating a New Malaria Vaccine Design that uses a Blood Stage P. falciparum Chassis for Non-Blood Stage Antigen Presentation
… and transgenic parasites that express the scaffold protein eTRAMP4 fused to either CSP or P25 were generated. We assessed the transgenic parasites for growth defects, proper fusion length, and localization to the parasitophorous vacuolar membrane. We also removed parasites from host red …
-
Investigating protrudin’s role in neuroprotection and axon regeneration in the central nervous system
Protrudin — a transmembrane scaffold protein found in tubular regions of the endoplasmic reticulum (ER) — has previously been shown to strongly promote neuronal survival and axon regeneration after central nervous system (CNS) injury. This is in part due to increased levels of integrins in the …
-
MES-DERIVED CARDIOMYOCYTES AS CELLULAR MODEL TO STUDY CARDIAC DISEASES: ALTERATIONS IN STRN AND GNB5 GENES
Striatin (Strn), a scaffold protein expressed in many tissues, among which in cardiomyocytes (CMs), whose altered expression has been found in various cardiac diseases. Here we studied the role(s) of cardiac STRN by comparing the electrophysiological properties of CMs, generated from STRN-KO and …
-
Regulation of Endocytosis of ROMK Channel by WNK Kinase Family
… Intersectin (ITSN) is a multimodular endocytic scaffold protein. The proline-rich domains of WNK1 and WNK4 bind with the Src-homology domain (SH3) of intersectin, and this interaction is important for the stimulation of endocytosis of ROMK by WNKs. Intersectin will further activate the GTPase …
-
Simulation and optimization tools to study design principles of biological networks
… of signal transduction networks based on a protein-domain network representation. This method generates models whose number of species, in the worst case, scales quadratically to the number of protein-domain sites and modification states, a tremendous saving over the combinatorial scaling in …
-
Models and analysis of yeast mating response : tools for model building, from documentation to time-dependent stimulation
… (Saccharomyces cerevisiae) have revealed many protein families and regulatory motifs also found in higher eukaryotes. This thesis develops several computational and experimental approaches that facilitate characterization of cellular signaling systems, and tests these approaches using yeast …
-
A Host Regulatory Circuit of HSV Gene Expression
… and latent cycles, where the immediate-early protein ICP0 plays a critical role in driving lytic replication and reactivation from latency. During this complex process, HSV-1 triggers STING, a host factor that inhibits viral infection. It is well established that STING mediates antiviral …
-
Cxcr2 Macromolecular Complex In Pancreatic Cancer: A Potential Therapeutic Target In Tumor Growth
… termini, which might interact with potential PDZ scaffold/adaptor proteins. We have previously reported that CXCR2 PDZ motif-mediated protein interaction is an important regulator for neutrophil functions. Here, using a series of biochemical assays, we demonstrate that CXCR2 is physically coupled …
-
Functional roles of butyrophilin 1a1 and xanthine oxidoreductase in lactating mammary gland
… in the cytoplasm, thus suggesting that both proteins may function in the accretion and/or secretion of milk-lipid droplets during lactation. Therefore, we investigated the potential functions of BTN and XOR in the lactating mammary gland of mice by molecular and cell biological approaches. …
Page 1 of 4