Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"scRNAseq"”.
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Embracing heterogeneity in multi-donor scRNAseq data: scalable inference of multi-scale heterogenity
Heterogeneity in gene expression is a characteristic of cell populations that has been linked to disease, development, tissue homeostasis, and immune function. Single cell RNA sequencing technologies have enabled researchers to study this heterogeneity in a genome-wide fashion. However, such …
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Multivariate Statistical Analysis In Single Cell Transcriptomics
… in the last decade, single cell RNA sequencing (scRNAseq) has emerged as an exciting weapon in the modern scientist’s arsenal to unravel cellular heterogeneity. The ability to measure the transcriptome in each individual cell in any given tissue poses challenges galore, ranging from purely …
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Systems and Comparative Analyses of Monocyte Dynamics Based Upon Single Cell Sequencing Data
… Furthermore, we analyzed publicly available scRNAseq datasets regarding monocytes from septic and severe COVID human patients and revealed a similar exhaustion phenotype as we documented in murine exhausted monocytes. In contrast, our analyses of newly published scRNAseq data regarding …
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A Context-Forward In Vivo Functional Genomics Platform For Target Discovery and Establishing Vulnerability Context In Pancreatic Cancer
… on a continuous scale. Additionally, through scRNAseq, this continuous scale was also observed intratumorally across PDX lines. Applying sgRNA direct-capture for targeted gene perturbation and sample multiplexing in scRNAseq, we validated PCEN-informed dependences (SMAD4, ZEB1, ILK) associated …
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Exploration of the malaria parasite lifecycle stages and strains circulating in natural infections at single-cell resolution
… development of single-cell RNA sequencing (scRNAseq) offers a great opportunity to study malaria parasites in natural infections and opens new avenues of exploration previously inaccessible to bulk approaches. In Chapter 1, I delve deeper into the motivations for using scRNAseq in natural …
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Pareto Task Inference Analysis of Single-Cell RNASequencing of Human Placenta Reveals Biological Insightsinto Adverse Pregnancy Outcomes
… contribute to APOs. Single-cell RNA sequencing (scRNAseq) approaches have characterized cellular populations and interactions at the maternal-fetal interface using traditional dimensionality-reducing methods such as UMAP-based clustering. However, these techniques may generate limited …
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Engaging Immune Cells within the Tumor Microenvironment to Enhance Efficacy of Oncolytic Virotherapy
… (TME), using single-cell RNA sequencing (scRNAseq) to investigate the infiltration and functionality within global populations or at the single-cell level. Our data show that FusOn-H3 can induce significant infiltration of both innate and adaptive immune cells. Detailed analysis by …
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Investigation of endogenous and adoptively transferred T cell function in a genetic mouse model of lung adenocarcinoma
… of tumor bearing mice using single cell RNAseq (scRNAseq).
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A novel antigen-receptor signalling reporter mouse reveals clonotype-independent intratumoral trapping as a spatial feature of CD8+ T cell exhaustion
… and clonality using single cell RNA sequencing (scRNAseq) to enable analysis of T cells at a clonal level. First, I generated robust protocols for the isolation and processing of EYFP+ cells to scRNAseq pipeline to overcome the challenge of preserving rare cells under time constraints. With these …
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On single-cell immune dynamics of chronic HIV infection and treatment in rhesus macaque models
… and therapeutics. Single-cell RNA sequencing (scRNAseq) is continually advancing our understanding of immune dynamics and when combined with well-characterized rhesus macaque models of HIV, provides an opportunity to profile immune perturbations over extensive time courses in a controlled …
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Single Cell Proteogenomics Analysis of AML Samples Treated with Decitabine and Venetoclax Reveals Divergent Treatment Escape Mechanisms
… with monocytic shifts. In one of these patients, scRNAseq additionally revealed a considerable presence of erythroid compartment upregulated with BCL2L1. Importantly, BCL2 was downregulated in all these compartments. One patient had displayed an erythroid shift during the nonresponsive stages of …
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Single Cell Analysis of B Cell Responses to Malaria
… in BCR affinity. Using single cell RNA sequence (scRNAseq), we observed that this mutation was not enriched in precursors to long lived plasma cells found within the early GC. However, this mutation was underrepresented in a population of precursor memory cells. Thus, we show that BCR affinity …
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Single Cell Analysis of B Cell Responses to Malaria
… in BCR affinity. Using single cell RNA sequence (scRNAseq), we observed that this mutation was not enriched in precursors to long lived plasma cells found within the early GC. However, this mutation was underrepresented in a population of precursor memory cells. Thus, we show that BCR affinity …
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Leveraging Single Cell Technologies For The Characterization and Treatment of Refractory Pancreatic Cancer
… (scCNVseq and snATACseq for DNA assays, scRNAseq for transcriptomics, and paired protein assays such as multiplexed immunofluorescence and fluorescence in situ hybridization for validation methods) to reveal inherent heterogeneity in assumptively isogenic pancreatic cancer (PDAC) cell …
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A PERTURB-SEQ SATURATION MUTAGENESIS APPROACH TO DISSECT THE MOLECULAR BASES OF GENETIC DISEASES
… to any disease-driving gene, we developed a scRNAseq-based platform. Through this approach, we could associate each cell (i.e., each variant) to a specific physiological or pathological activity based on a specific transcriptional signature. This approach could represent a milestone in the …
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An in vitro cell lineage model to identify alterations in the differentiation program of neuroblastoma
… in the available single-cell RNA sequencing (scRNAseq) datasets of both normal and tumoral human AM. These new cellular differentiation systems will provide us a unique tool to study in depth the role of the genomic alterations in NB pathogenesis.
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Investigating the coordination of germline stem cell fate by ecdysone signaling and BMP signaling in the Drosophila ovary
… We perfomed single-cell RNA sequencing (scRNAseq) to assess the potential transcriptomic differences between the three tumor genotypes. UMAP distribution and differential gene expression show that the over-expression of EcR.B1 and constitutively active tkv (TkvACT) yield undifferentiated …
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Elucidating Astrocytic Diversity in Human Cerebral Organoids
… markers from single-cell RNA sequencing (scRNASeq) results, fluorescence-activated cell sorting (FACS)-based isolation of these populations reveal that they differ in morphology, calcium signalling, synaptogenic and synaptic engulfment potential, consistent with their transcriptome. In …
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MULTIPLE MYELOMA PLASMA CELL PROFILING: INTEGRATED SINGLE-CELL RNA AND VDJ SEQUENCING APPROACHES UNCOVER NOVEL CLINICAL INSIGHTS
… sequenziamento a singola cellula di RNA e VDJ (scRNAseq e scVDJseq) per esplorare in dettaglio i paesaggi genetici e trascrittomici del MM a livello di singola cellula, con particolare enfasi sulle pPCs, sulle interazioni con il TME e sull’evoluzione clonale. Il primo risultato principale di …
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