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Showing 1 to 20 of 29 for “"scFvs"”.
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… single chain variable region fragments (scFvs), which like full-size mAbs, are also capable of specific antigen-binding. The constant and rapidly expanding use of antibodies and their derivatives presents a need for a fast and effective method of production. Traditionally, antibodies have …
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Towards an antibody mediated resistance against Geminiviruses
In this study, single chains (scFvs) against key proteins of the Tomato leaf curl virus-India were generated using phage display and/or hybridoma technologies. Purified AV1 (coat protein) and AC1 (replicase enhancer) viral proteins were used for mice immunization and screening of phage display …
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Antikörper vermittelte Fusarien-Resistenz in transgenen Pflanzen
… against surface structures of Fusarium. These scFvs were be fused to antifungal peptides and proteins (AFP) and expressed in plants and should mediate resistance against the pathogenic fungi in planta. To generate scFv antibodies chicken were immunized with different fungal …
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Engineering intracellular antibody libraries
… were generated; the genes expressing the scFvs were constructed and the CDRs were randomized using PCR amplification. Genes expressing scFvs were cloned, using the homologous gap repair mechanism in Saccharomyces cerevisiae. Representative members of scFv libraries were sequenced to …
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Herstellung und Charakterisierung von rekombinanten Antikörpern und scFv Fusionsproteinen zur Generierung Phytophthora infestans-resistanter Kartoffelpflanzen
… AFP-scFv fusion protein. To generate suitable scFvs two strategies were used. On the one hand spleen cells of mice that have been hyperimmunized with cell wall fragments of P. infestans were fused with myeloma cells. This approach yielded twelve P. infestans specific monoclonal antibodies …
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The Production of Recombinant Immunoglobulin Superfamily Proteins in the Yeast Saccharomyces Cerevisiae
Although scFvs and scTCRs are structurally homologous, they have very distinct expression and stability behaviors. In fact, in order to develop an efficient scTCR production strategy like that developed for scFvs, it was necessary to alter the characteristics of the protein itself to improve its …
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In vitro diversification of chimeric human/chicken antibodies
… antibodies. Human antibodies in the form of scFvs expressed in the surface of these cells have been successfully diversified to produce a scFv library, from which antibodies of desired specificity and improved affinity can be evolved using a combination of flow cytometry (FACS) and magnetic …
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Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… analyses revealed selection of two and four scFvs specific to ADC and ODC, respectively. Electromobility shift assays confirmed the functionality of the affinity purified scFvODC1 and scFvODC3. The binding properties of scFvODCs on soluble antigen were further verified by solution competition …
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Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies
… different anti-LDH and six different anti-HRPII scFvs were identified by sequence analysis. Evidence of high levels of antigen-driven gene conversion events was found in the framework and complementary determining regions and the VL chain pseudogene donors were identified. Stability of the …
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Generation and characterization of single chain antibody fragments binding to the coat protein of grapevine leafroll associated virus 2 and 3
… The rationale of this work was to generate scFvs by recombinant DNA technology that specifically recognize and inactivate the coat protein of the GLRaV-2 and 3 and therefore potentially inactivate virus disassembly and virus spreading. The constitutive expression of these scFvs may create …
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Production and characterisation of novel recombinant antibody fragments against sialic acid
… serum albumin). Anti-sialic scFvs were isolated byphage-display and binding activity, multimeric status, and multivalent properties wereassessed by ELISA, HPLC, FPLC, and SPR. One clone, AE8, displayed the strongest reactivity to a panel of different sialylated structures. In …
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Engineering of recombatinant antimalria antibodies for application in paratransgenesis
… length, in the VH-VL and VL-VH orientation. The scFvs were cloned into different expression plasmids to evaluate a suitable expression system. The orientation of the variable domains on secretion of the scFv 4B7 was investigated. No secretion was observed for the scFv 4B7 in the VH-VL …
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Characterization of single-cycle flavivirus particles for use as a vaccine to prevent West Nile disease and to examine immune responses to flavivirus infection
… of animals inoculated with luciferase-expressing SCFVs revealed 1000-fold greater bioluminescence in the absence of a type-I IFN response. The affect of this IFN response on gene expression was dramatic, but short lived and did not appear to play a role in SCFV persistence, as SCFV gene expression …
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Multi-Pronged Approach to Understanding the Toxicity Associated with CAR T-Cell Therapy
… were tested, including secretion of eotaxin-3, scFvs, and a nanobody. While eotaxin-3 and anti-CCR2 scFvs showed limited efficacy, anti-CCL2 constructs were expressed and bound their target, though migration blocking was not reproducible. These studies nevertheless demonstrate proof-of-principle …
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Herstellung, Expression und Charakterisierung von rekombinanten Antikörpern und Immuntoxinen gegen Phoma lingam, Sclerotinia sclerotiorum und Verticillium dahliae
… specific and of high affinity. All of the scFvs were expressed as functional antibody fragments in bacteria and plant leaves. The antibodies were characterized in vitro (ELISA and Immunoblot) and in vivo (Immunofluorescence microscopy) for their capacity to bind antigen and no significant …
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NOVEL MONO-, BI- AND TRI-SPECIFIC IMMUNOMODULATORY ANTIBODIES FOR CANCER THERAPY AND TOOLS FOR OTHER THERAPEUTIC AND DIAGNOSTIC APPLICATIONS
… an IC (PD-L1, PD-1 or LAG-3) by including the scFvs derived from the mentioned novel mAbs. We found that the novel TCEs enhanced cytokine release from hPBMCs, with a strong secretion of IL-2 and IFNγ and showed more potent antitumor effects than the combination of the parental bispecific …
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Synthesis and biological evaluation of auristatin-F recombinant antibody-drug conjugates for cancer therapy
… antibody single-chain variable fragments (scFvs) exhibiting specificity towards the epidermal growth factor receptor (EGFR), chondroitin sulfate proteoglycan receptor (CSPG4) and CD44 receptors more highly expressed in various cancer types. The design and successful synthesis of two …
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Pretargeted cancer radioimmunotherapy and radioimaging using antibodies and antibody fragments
… revealed a moderate tumor uptake of radiolabeled scFvs with significant accumulation in the liver and kidneys. The phage display derived single-chain scFv fragments against the TF antigen demonstrated potential for development as an imaging agent but requires more work to achieve favorable …
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Development of a Dusky kob scFv gene phage display library for the discovery of antibodies to Brome mosaic virus - a proxy for a novel, emerging fish pathogen
… this study, a phage display library displaying scFvs derived from combination pairings of Dusky kob (Argyrosomus japonicas) variable heavy and light chain fragments, sourced from the splenic B cells of healthy Dusky kob, was constructed. The library was subjected to two rounds of biopanning …
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