Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"scFv antibodies"”.
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Engineering scFv Antibodies for Point-of-Care Diagnostics
… CysC-specific single chain variable fragment (scFv) antibodies with a cellulose binding tag (CipA) to tether it to paper, and a fluorescent protein (mCherry) to provide a detectable signal, the aim is to develop a paper-based sandwich immunoassay that can be used at bedside for faster results. …
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Development of monoclonal antibodies targeting T cell coinhibitory proteins TIM-3 and TIM-4
Immune checkpoint inhibition (ICI) therapy uses antibodies to block checkpoint proteins from binding with their partner proteins to help overcome dysfunctional T cells and promote the recognition and elimination of tumour cells. The exploration of novel immune checkpoint targets, like T cell …
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Molecular Engineering of a Single-Chain FV Antibody Fragment to Femtomolar Affinity
Mutants of anti-fluorescein scFv antibody 4-4-20 improved more than 1000-fold with respect to ligand dissociation kinetics and equilibrium binding affinity have been isolated from mutagenized libraries displayed on yeast and screened by flow cytometry. Analysis of the nature of mutations conferring …
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Generierung, Charakterisierung und funktionaler Assay von Antikörpern und Antikörperfragmenten gegen die HCV Strukturproteine core, E1 und E2
… carcinoma. In the dissertation on hand, antibodies against the Hepatitis C (subtype 1b) structure proteins core, E1 and E2 were synthesised. The coding sequences of full-length and deleted versions of the protein mentioned above served as starting material. In a first step sufficient …
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Nanofabrication towards biophotonics
… before a bioassay using whole molecule human IgG antibodies and antigen is developed and performed on the senor. In chapter 3 the fabricated sensors are integrated into a custom built system, capable of real time, multiplexed detection of biomolecules. Here, scFv antibodies of two biomolecules …
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Antikörper vermittelte Fusarien-Resistenz in transgenen Pflanzen
… as well as costs. The use of recombinant antibodies in transgenic plant to generate fungal pathogen resistant plants is a promising way to solve these problems in a consumer-friendly way. The goal of this work was to generate scFv antibody fragments against surface structures of Fusarium. …
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Studies on voltage-gated sodium channel β3 subunit structure and cancer-related functions using single-chain variable fragment antibodies and bioinformatics
… the use of single-chain variable fragment (scFv) antibodies to study the binding site of the β3 subunit on the pain-sensing channel, Nav1.7, and investigate the role of the VGSC β3 subunit in cancer guided by bioinformatic analysis. *In silico* docking was used to construct a binding model …
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Structural and functional role of the extracellular loops and C-terminal domain of the voltage-gated sodium ion channel
… library of single chain fragment variable (scFv) antibodies, specific binders to the CTD of Nav1.5 and Nav1.7, were found and purified. These scFvs could have gating effects on their Nav channel targets, which might prove therapeutically applicable.
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Development and Characterization of recombinant immunotoxins for cervical carcinoma
… for cancer. Immunotoxins utilize the ability of antibodies to target cancerous cells without affecting the healthy cells due to certain surface receptors being overexpressed in cancerous cells and not in healthy cells. Studies have shown that cell surface receptors LGR5, EpCAM and CD90 are …