Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 120 for “"scFv"”.
-
Engineering scFv Antibodies for Point-of-Care Diagnostics
… CysC-specific single chain variable fragment (scFv) antibodies with a cellulose binding tag (CipA) to tether it to paper, and a fluorescent protein (mCherry) to provide a detectable signal, the aim is to develop a paper-based sandwich immunoassay that can be used at bedside for faster results. …
-
Construction and mutagenesis of an anti-insulin single chain Fv (scFv) using protein engineering techniques: Identification of peptide mimotopes which inhibit scFv-insulin binding.
… as V(H) and V(k). A single chain Fv (scFv) was constructed using a polymerase chain reaction (PCR) assembly technique from the anti-insulin V(H) and V(k). The scFv demonstrated similar, but not identical binding properties to the parental anti-insulin antibody. Binding of four of six …
-
Herstellung und Charakterisierung von rekombinanten Antikörpern und scFv Fusionsproteinen zur Generierung Phytophthora infestans-resistanter Kartoffelpflanzen
… specific single chain antibody fragments (scFv) and genetically fuse them to antifungal peptides (AFP) in order to establish transgenic potato plants that are resistant against P. infestans by expressing the AFP-scFv fusion protein. To generate suitable scFvs two strategies were used. On …
-
Targeting of adenovirus serotype 5 infectivity to EGFRvIII-expressing cells using a bispecific CAR-scFv.
… single chain antibody variable domain fragment (scFv), is able to bind with high affinity and specificity to EGFRvIII and not to the wild-type receptor. The purpose of this study was to investigate a possible means of developing an adenoviral vector (based on subclass C viruses) that can utilize …
-
Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against …
-
Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we studied for cell surface receptor of …
-
INVESTIGATION OF LIGAND SIZE ON TARGETED PLGA NANOPARTICLES FOR HER2 BREAST CANCER: TRASTUZUMAB AND ITS ScFv ANTI-HER2 ANTIBODY
… IgG (TrAb) or single chain fragment variable (ScFv), were decorated on the PLGA NPs surface and evaluated in terms of their ability to target HER2 breast cancer cells. We observed the effects of these NPs against different cell lines (MCF-7 and SK-BR-3). Thus, ligand modified structurally …
-
Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
3D8 single chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and …
-
Antiviral activity of 3D8 single chain Fv against RNA virus infection
… that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it localizes in cytosol resulting in cell death by cellular rRNA degradation. These result mean that 3D8 scFv would also have potential activity as an …
-
Konstruktion und Charakterisierung von rekombinanten Immuntoxinen zur Therapie von metastasierendem Pankreaskarzinom
… First of all the recombinant immunotoxin 425(scFv)-ETA’ was generated by genetically fusing the anti-EGFR single chain fragment 425(scFv) to a truncated mutant of Pseudomonas exotoxin A (ETA’). Using the expression vector pBM1.1, functional 425(scFv)-ETA’ was periplasmically expressed under …
-
Development of recombinant antibody mediated resistance against Tomato yellow leaf curl virus
… study, we exploited the expression of specific scFv fragment in plant cells for suppression of disease symptoms caused by TYLCV infection. The C1, V1 and V2 genes encoding Rep, CP and MP, respectively, were cloned into the TOPO vector and subsequently into pGEX-5x3 and pMALc2x expression …
-
Antikörper vermittelte Fusarien-Resistenz in transgenen Pflanzen
… way. The goal of this work was to generate scFv antibody fragments against surface structures of Fusarium. These scFvs were be fused to antifungal peptides and proteins (AFP) and expressed in plants and should mediate resistance against the pathogenic fungi in planta. To generate scFv …
-
Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… on the function of an antibody, 3D8 scFv (single chain variable fragment; variable region of heavy chain connected to variable region of light chain by (G4S1)3 linker) which has DNA-binding/-hydrolyzing, heparin-binding and cell-penetrating activities. We generated single …
-
Antibody engineering for cancer therapy
… and used to construct an AF-20 single-chain Fv (scFv). The AF-20 scFv was shown to bind specifically to the same epitope as mAb AF-20 with a binding affinity of 4nM. The AF- 20 scFv was also internalized into tumor cells in a manner identical to that of the original mAb AF-20. The scFv was later …
-
Towards an antibody mediated resistance against Geminiviruses
In this study, single chains (scFvs) against key proteins of the Tomato leaf curl virus-India were generated using phage display and/or hybridoma technologies. Purified AV1 (coat protein) and AC1 (replicase enhancer) viral proteins were used for mice immunization and screening of phage display …
-
Engineering of recombatinant antimalria antibodies for application in paratransgenesis
… formatted as single chain variable fragments (scFv). Synthetic genes encoding the scFv 4B7 and Pf-NPNA-1 were constructed, with varying linker length, in the VH-VL and VL-VH orientation. The scFvs were cloned into different expression plasmids to evaluate a suitable expression system. The …
-
Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …
-
Engineering intracellular antibody libraries
… affect single chain variable fragment (scFv) binding capacity. The parameters that were varied include the number of variable complementarity determining regions (CDRs), the number amino acids used to diversify CDRs, and configuration of the structure. How the parameters affect the …
Page 1 of 6