Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 105 for “"scFv"”.
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Engineering scFv Antibodies for Point-of-Care Diagnostics
… CysC-specific single chain variable fragment (scFv) antibodies with a cellulose binding tag (CipA) to tether it to paper, and a fluorescent protein (mCherry) to provide a detectable signal, the aim is to develop a paper-based sandwich immunoassay that can be used at bedside for faster results. …
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Construction and mutagenesis of an anti-insulin single chain Fv (scFv) using protein engineering techniques: Identification of peptide mimotopes which inhibit scFv-insulin binding.
… as V(H) and V(k). A single chain Fv (scFv) was constructed using a polymerase chain reaction (PCR) assembly technique from the anti-insulin V(H) and V(k). The scFv demonstrated similar, but not identical binding properties to the parental anti-insulin antibody. Binding of four of six …
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Targeting of adenovirus serotype 5 infectivity to EGFRvIII-expressing cells using a bispecific CAR-scFv.
… single chain antibody variable domain fragment (scFv), is able to bind with high affinity and specificity to EGFRvIII and not to the wild-type receptor. The purpose of this study was to investigate a possible means of developing an adenoviral vector (based on subclass C viruses) that can utilize …
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Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against …
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Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we studied for cell surface receptor of …
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INVESTIGATION OF LIGAND SIZE ON TARGETED PLGA NANOPARTICLES FOR HER2 BREAST CANCER: TRASTUZUMAB AND ITS ScFv ANTI-HER2 ANTIBODY
… IgG (TrAb) or single chain fragment variable (ScFv), were decorated on the PLGA NPs surface and evaluated in terms of their ability to target HER2 breast cancer cells. We observed the effects of these NPs against different cell lines (MCF-7 and SK-BR-3). Thus, ligand modified structurally …
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Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
3D8 single chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and …
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Antiviral activity of 3D8 single chain Fv against RNA virus infection
… that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it localizes in cytosol resulting in cell death by cellular rRNA degradation. These result mean that 3D8 scFv would also have potential activity as an …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… on the function of an antibody, 3D8 scFv (single chain variable fragment; variable region of heavy chain connected to variable region of light chain by (G4S1)3 linker) which has DNA-binding/-hydrolyzing, heparin-binding and cell-penetrating activities. We generated single …
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Antibody engineering for cancer therapy
… and used to construct an AF-20 single-chain Fv (scFv). The AF-20 scFv was shown to bind specifically to the same epitope as mAb AF-20 with a binding affinity of 4nM. The AF- 20 scFv was also internalized into tumor cells in a manner identical to that of the original mAb AF-20. The scFv was later …
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Engineering of recombatinant antimalria antibodies for application in paratransgenesis
… formatted as single chain variable fragments (scFv). Synthetic genes encoding the scFv 4B7 and Pf-NPNA-1 were constructed, with varying linker length, in the VH-VL and VL-VH orientation. The scFvs were cloned into different expression plasmids to evaluate a suitable expression system. The …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …
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Engineering intracellular antibody libraries
… affect single chain variable fragment (scFv) binding capacity. The parameters that were varied include the number of variable complementarity determining regions (CDRs), the number amino acids used to diversify CDRs, and configuration of the structure. How the parameters affect the …
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Generation and therapeutic evaluation of recombinant immunotherapeutics on CD64+ monocytic cell lines and polarized ex vivo differentiated human macrophages
… The CD64-targeting antibody fragment (H22(scFv)) was used to generate; 1) a recombinant immunotoxin (rIT) through genetic fusion with the enzymatic domain of Pseudomonas aeruginosa exotoxin A (ETA`), or 2) a SNAP-tag fusion protein (FP) to allow chemical coupling of the antibody fragment to …
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Characterization of anti-DNA antibody with Tat peptide sequence in CDR
… 3D8 단일 가닥 가변부위 (single chain variable fragment: scFv)는 핵산결합능 및 가수분해활성이 있으며, 3D8 VL 단독부위만으로도 핵산 결합능 및 가수분해능을 발휘한다. 또한 3D8 scFv는 세포막 침투능이 있어서, 세포막을 투과한 후, 세포질에 위치하게 되고, 결국은 세포사멸을 유도한다고 알려져 있다. 본 연구에서는 3D8 scFv의 핵산가수분해 활성은 그대로 유지하면서, 세포막 투과 후 핵으로 이동할 수 있는 3D8 scFv 변이체를, CDR 교체 방법으로, 제작하고자 하였다. 핵으로 …
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Production and characterisation of novel recombinant antibody fragments against sialic acid
… antibodies.A single-chain antibody fragment (scFv) library was constructed from a chicken that was immunised with a novel synthetic sialic acid protein-conjugate (Neu5Gchumanserum albumin). The scFv library was biopanned using a second novel sialoneoglycoconjugate(Neu5Gc-bovine serum albumin). …
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Rekombinante Antikörper gegen tumorassoziiertes MUC1
… single chain Fv-Antikörperfragmente (scFv-Fragmente) mit spezifischer Bindung an die VNTR-Region von tumorassoziiertem MUC1 gewonnen werden. Ausgehend von cDNA, die aus den peripheren Lymphozyten von mit einem synthetischen MUC1-Glykopeptid immunisierten Mammakarzinom-Patientinnen …
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Generation of recombinant SNAP-tag based antibody fusion proteins for immunotherapy of acute myeloid leukemia (AML)
… comprised of single-chain fragment variables (scFv) fused to a self labelling human protein called SNAP-tag were generated. SNAP-tag was used for site-specific conjugation to O6 -benzylguanine (BG) derivatives to demonstrate functionality against overexpressed and therapeutically relevant AML …
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