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Showing 1 to 7 of 7 for “"saposin"”.
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Structural and Functional Studies of the Human Saposin Proteins
The human saposins are four homologous activator proteins that are essential for the lysosomal degradation of sphingolipids (SLs) with small headgroups. They function in part to increase the solvent accessibility of these SLs to specific acid hydrolases by two proposed modes of action. In the …
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Molekulare Charakterisierung Saposin-ähnlicher Proteine von Entamoeba histolytica SCHAUDINN
Saposin-ähnliche Proteine (SAPLIPs) sind membraninteragierende Proteine, die sich durch die konservierte Position von drei Disulfidbrücken, einer typischen alpha-helikalen Proteinfaltung und der Fähigkeit mit Lipiden zu interagieren, auszeichnen. Ihre zellulären Funktionen sind äußerst vielfältig. …
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Modelling saposin deficiency in Drosophila: progressive neurodegeneration, storage and physiological decline
Saposin deficiency is a lysosomal storage disorder (LSD) characterised by the lysosomal accumulation of sphingolipids. The disorder is caused by mutations in the prosaposin gene, which encodes 4 activator proteins: saposins A - D. Mutations affecting individual saposins lead to different LSDs. …
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Development of a saposin A based native-like phospholipid bilayer system for NMR studies
… structure and function. The recently developed saposin-A (SapA) based lipid nanoparticle system seems to be advantageous over existing membrane mimetic system. It provides a native-like lipid bilayer, high incorporation yield and more importantly size adaptability. SapA lipid nanoparticles have …
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Protein-sphingolipid interactions and their role in immunity and disease
… by the lysosomal lipid transfer protein Saposin B, but the lipid loading mechanism is unknown. I used a range of highly sensitive biochemical assays to probe the interaction between CD1d and SapB. Interestingly, no direct interaction was detectable. Importantly, this suggests that …
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Mechanism of anaesthetic activation, combination and antagonism
… such as membrane-scaffold protein (MSP)-, saposin-, and SMALP nanodiscs. Antibody fragments have been used in the past to support particle alignment and distribution in cryo-EM specimen. On the example of several cryo-EM structures, I will discuss the impact and necessity of such fiducials. …
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Molecular mechanism of Trypanosoma brucei Aquaglyceroporin 2
… proteins. Subsequent reconstitution into saposin A-lipid nanoparticles was essential to stabilise TbAQP2 and TbAQP2X3 sufficiently in a buffer compatible with cryo-EM structural studies. Cryo-EM sample preparation of TbAQP2, TbAQP2X3 and the TbAQP2-drug complexes was optimised and suitable …