Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"sRNAs"”.
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Helicobacter pylori gene regulation by virulence region located sRNAs
… and regulate gene expression. Several sRNAs were identified in the cagPAI, and, to date, only one has been characterized. To learn more about cagPAI sRNAs and the genes they regulate, I characterized transcriptional regulatory sequences of two cagPAI sRNAs, HPnc2620 and HPnc2665, and …
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Characterization of Helicobacter pylori sRNAs HPnc2525, HPnc2600, and HPnc2645
… and other bacteria have found that small RNAs (sRNAs) play a role in gene regulation. These transcripts are 50-300 nucleotides in length and act independently on expressed targets. sRNAs serve to regulate and fine-tune gene expression by interacting with target mRNA molecules to inhibit or …
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Identification and characterization of novel small RNAs in Desulfovibrio vulgaris Hildenborough
… to identify novel, small, regulatory RNAs (sRNAs)--an important class of cellular regulators that have yet to be studied in <italic>D. vulgaris</italic>--and to determine their effect on cellular processes within the organism. Computational and transcriptomic high-throughput sequencing …
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Rickettsia prowazekii and its ‘Junk DNA’: The Identification and Characterization of Small RNAs
… and growth called bacterial small RNAs (sRNAs). These sRNAs are classified as either trans-acting, whose biogenesis is predominantly linked to intergenic regions, or cis-acting, encoded on the antisense strand of an open reading frame (ORF). Despite being present in a majority of other …
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Multiple virulence genes and other MRNA regulatory targets are differentially expressed in two Helicobacter pylori strains overexpressing small RNAs
… proteins in H. pylori, its wealth of small RNAs (sRNAs) may also regulate mRNAs, including in the cagPAI. The nascent body of work in H. pylori sRNAs supports consequential roles for posttranscriptional regulation by sRNAs, and the clinical relevance of the cagPAI further suggests these for …
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Biogenesis of Y RNA–derived small RNAs
Small non-coding RNAs (sRNAs) constitute a significant portion of the transcriptome in eukaryotes. Many of these sRNAs regulate gene expression. Next-generation sequencing (NGS) has revealed a plethora of previously uncharacterised sRNAs with potential biological function, a number of which …
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Characterizing the AbcR/VtlR system in the Rhizobiales
… orders within the Alphaproteobacteria. The AbcR sRNAs are an example of sibling sRNAs, where two copies of the abcR gene are typically present in the genome. The AbcRs are involved in the negative regulation of ABC-type transport systems, which are important components for nutrient acquisition. …
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Computational prediction of RNA-based gene regulatory mechanisms in human and Tetrahymena
… of gene regulation mediated by small RNAs (sRNAs) is just beginning to come into focus. RNA interference (RNAi) pathways have been shown to mediate processes such as genomic rearrangement in ciliates and developmental timing and tissue differentiation in plants and animals. Here we present a …
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Studying the riboregulatory role of Hfq in Klebsiella pneumoniae using CLASH (crosslinking, ligation and sequencing of hybrids)
… in PTR (Fig A). As the transcription of numerous sRNAs is regulated by transcription factors (TF), I explored the link between a TF - “RamA” and its influence on Hfq bound sRNAs in mediating antibiotic resistance (ABR). My analyses between Ecl8 and Ecl8ΔramR provided insights of the …
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Student Registered Nurse Anesthetists' Perceptions of Bullying and its Impact on Learning
… among anesthesia preceptors, and if so, how SRNAs perceive bullying has affected their educational experience. Methods: The American Association of Nurse Anesthetists (AANA) disseminated an online survey based on an existing tool to SRNAs for this study. The study used a quantitative …
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Initiation of translation of the small RNA of the cowpea strain of tobacco mosaic virus
… of initiation of translation of mutant tCcTMV-SRNAs showed that the region of the tandem AUG's must contain some feature which directs the ribosome to initiate translation within that region and that the tandem AUGs themselves may be important for the actual selection of the correct translation …
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A combinatorial approach for improved target prediction, validation, and characterization of small RNAs in Escherichia coli
Bacterial small RNAs (sRNAs) are involved in regulating targets involved in various processes in the cell, including stress responses and metabolic processes. As RNA regulators that are degraded rapidly, they are ideal for fine-tuning gene expression in response to rapid environmental changes. Even …
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Quality checking and expression analysis of high-throughput small RNA sequencing data
… identi�cation of small non-coding RNAs (sRNAs) and to assess their regulation using di�erential expression analysis by comparing two or more di�erent conditions. During an analysis of a typical set of sRNA sequencing (sRNA-seq) libraries, a large variety of tools and methods are used on …
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Small RNAs within the developing seed of Arabidopsis thaliana
… we were able to assay the parent-of-origin for sRNAs within the seed tissues. Although sRNA parent-of-origin genome-wide reflects the overall genomic complement from the parents, there are regions of the genome with strong parental bias in sRNA expression, and endosperm DSRs are enriched for …
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Discovery and Characterization of a Novel Regulatory Small RNA, VcrS, Required for Virulence in Brucella abortus
… through the use of small regulatory RNAs (sRNAs). sRNAs bind to messenger RNA (mRNA) targets via complementary base pairing. sRNAs are a class of regulatory molecules in bacteria that elicit rapid post-transcriptional regulation. sRNA-mRNA binding can positively or negatively influence gene …
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Characterization of Small Non-Coding RNAs in the Seminal Plasma of Beef Bulls with Predicted High and Low Fertility
… relationship between expression of these sRNAs and fertility in beef bulls and to characterize piRNA expression in bull seminal plasma. Semen samples were collected from 35 beef bulls and analyzed for Arachis hypogaea/peanut agglutinin (PNA) and Lens culinaris agglutinin (LCA) scores. PNA …
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Roles of Small Rnas and Paralogous Proteins In Bacillus Anthracis Virulence Gene Regulation
… through the function of small regulatory RNAs (sRNAs). Small regulatory RNAs (sRNAs) are short transcripts that base-pair to mRNA targets or interact with regulatory proteins. sRNA function has been studied extensively in Gram-negative bacteria; comparatively less is known about sRNAs in …
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Implementation of an evidence-based wellness program for student registered nurse anesthetists
Student Registered Nurse Anesthetists (SRNAs) face many challenges throughout their rigorous 36-month educational program. Many students report increasing levels of stress and a reduction in overall wellbeing. While the American Association of Nurse Anesthetists provides supportive resources in the …
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Small RNA and genome interactions in Chlamydomonas reinhardtii recombinants
… relying on 20Q24nt single stranded small RNAs (sRNAs). Small RNAs, due to their ability to set up persistent epigenetic marks at a locus, have the potential to create heritable transgressive gene expression. For example, when genetic variation from one parental genome presents novel targets to …
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Integration of small RNAs in the control of Salmonella pathogenicity island 1(SPI1)
… and genetic analyses, I identified a number of sRNAs that feed into hilD or hilA translation to contribute to SPI1 expression. Among them, I demonstrate that two oxygen-dependent sRNAs, FnrS and ArcZ, repress hilD translation. Genetically, the sRNAs base pair with hilD mRNA to regulate …
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