Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"rpos"”.
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A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi
… RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by …
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A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi
… RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by …
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On the coupling of noisy processes in biology to produce functional phenotypic variability
… expression of a key stress response regulator, RpoS, allows $\textit{E. coli}$ to modulate its noisy growth dynamics to survive future adverse environments. We first demonstrate that single cells in bulk, exponential phase cultures have heterogeneous $\textit{rpoS}$ expression. Combining …
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An Investigation of the Genetic Control of Biofilm Formation in Bacteria (E. coli K-12 MG1655)
… morphogene bolA and RNA polymerase sigma factor (rpoS) in biofilm formation. Secondly, the effect on respiratory activity of E. coli in presence and absence of these two genes and thirdly, the adherence pattern and formation of biofilm by E. coli on stainless steel, polypropylene and silicon …
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RURAL POLITICAL AGENCY AND LOCAL POLITICS IN GLOBALIZED MARKET
… participation in Rural Producer Organizations (RPOs), popular community-based farmer organizations, which have proliferated over the last decade. Two research questions guide this project: (1) What are the conditions that explain rural participation in RPOs in Indonesia? and (2) What are the …
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Coping with stress: How Reactive Oxygen and Nitrogen Species alter the transcriptional landscape of the Lyme disease spirochete Borrelia burgdorferi
… via western blotting. Genes regulated by RpoS (including those associated with mammalian infection such as ospC, dbpA, and bba66) were downregulated in response to sublethal concentrations of H2O2 (25 µM) and sper/NO (0.625 mM) coupled with a pH shift—the expression patterns were less …
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A Molecular Genetic Investigation of the Regulation of Outer Surface Protein C in Borrelia Burgdorferi: Identification and Characterization of the Novel Regulatory Protein BBD18
… It has been shown that the central Rrp2/RpoN/RpoS pathway is essential for the upregulation of OspC and other genes important for mammalian infection. However, many key aspects in the strict regulation of OspC remain undefined. In order to investigate the complex regulatory mechanisms …
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A Molecular Genetic Investigation of the Regulation of Outer Surface Protein C in Borrelia Burgdorferi: Identification and Characterization of the Novel Regulatory Protein BBD18
… It has been shown that the central Rrp2/RpoN/RpoS pathway is essential for the upregulation of OspC and other genes important for mammalian infection. However, many key aspects in the strict regulation of OspC remain undefined. In order to investigate the complex regulatory mechanisms …
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The effects of nutrient limitation and growth rate on the regulation of the multi-drug pump genes acrAB in escherichia coli
… stationary phase in the batch culture of an rpoS mutant. In the same rpoS mutant it was found that there was an even greater derepression of acrR transcription. When rpoD was overexpressed from an inducible multicopy plasmid, it was determined that the repression by o was not through …
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The effects of low-shear modeled microgravity on Streptococcus pneumoniae and adherent-invasive Escherichia coli
… of wild-type AIEC strain O83:H1 and an isogenic rpoS mutant (CAA001), after growth under LSMMG, revealed alterations in environmental stress resistance and increased adherence. Altered resistances to thermal and osmotic stresses were observed by LSMMG-grown AIEC O83:H1, while resistance to …
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Estado viável não cultivável em Salmonella enterica sorovar Enteritidis deficiente na síntese de (p)ppGpp
… dos genes mreB, relacionado ao citoesqueleto e, rpoS, que codifica genes de resposta ao estresse, de estirpes mutantes simples e duplo da Salmonella Enteritidis PT4 578 deficientes na síntese de (p)ppGpp, em razão de mutação no gene relA, que codifica uma GTP pirofosfoquinase, e,ou spoT, que …
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Posttranscriptional and posttranslational regulation of virulence in erwinia amylovora
… in delayed disease progress. Highly accumulated RpoS proteins were detected in the clpXP mutant, and mutation of rpoS in the clpXP mutant background restored virulence to the wild-type level. These suggest that ClpXP-dependent RpoS degradation positively affects virulence traits. In addition, …
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Influence of Physiological State, Prolonged Dry Storage, and Passage through Simulated Digestion on the Survival and Gene Expression of Salmonella enterica sv. Tennessee
… to desiccation and gastric stresses. The purpose of this study was to quantify the effect of physiological state (planktonic versus biofilm) and prior exposure to desiccation on Salmonella survival and gene expression after passage through an in-vitro digestion model. Cells of Salmonella …
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Superoxide Toxicity in Escherichia Coli
… role. We found that sodC is activated by RpoS in stationary phase, is repressed by Fnr and ArcA anaerobically, and is repressed when copper is unavailable. Mutants of sodC in E. coli and S. typhimurium suffer damage during aerobic growth from a cellular source of superoxide. The unknown …
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Molekulare Analyse des probiotischen Stamms Escherichia coli Nissle 1917
… bei EcN durch den alternativen Sigma-Faktor RpoS, der im Gegensatz zu rpoS-Deletionsmutanten zu einer gesteigerten mRNA-Expression des Tight-junction Proteins ZO-1 führt. Des Weiteren führte die Untersuchung von EcN-Deletionsmutanten zu der Schlussfolgerung, dass einige genomische Inseln für …
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Functional analysis of the role of alternative sigma factors in Vibrio parahaemolyticus host pathogen interactions
… All studied species possess a single copy of RpoS, however 22 species possess 1 to 3 additional copies of a divergent RpoS-like sigma factor. The greatest amount of diversity is within the ECF subfamily; here we demonstrate there are 3 highly conserved ECFs, which include RpoE, and several …
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Stress And Survival Of Enterobacteriaceae: Studies Of Dehydration Tolerance And Polymyxin Resistance
… DksA and the general stress response regulator RpoS. In addition to studying dehydration tolerance, we investigate resistance to polymyxins, which are cationic antimicrobial peptides used as last-resort antibiotics in Gram-negative bacteria. The polymyxin resistance network differs in network …
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Calcium homeostasis in Escherichia coli
… transposon mutants and by 10-fold induction of rpoS in elevated calcium condition in mutants defective in calcium transport. The results also reveal calcium-regulation of yfaD and ybbO, however, their functions are yet unknown. Cumulatively, a comprehensive picture emerges wherein it seems …
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Untersuchungen zum Einfluss der ATP-abhängigen Protease ClpXP auf die Regulation und Expression Virulenz-assoziierter Gene des uropathogenen E. coli Stammes 536
… Regulatoren wie dem alternativen Sigmafaktor RpoS kommt die regulatorische Funktion der Protease auf post-translationaler Ebene zum Tragen. Um den Einfluss der Protease ClpXP auf die Virulenz-eigenschaften uropathogener Escherichia coli zu studieren, wurde der uropathogene E. coli Stamm 536 …
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Identification and Characterization of SitABCD, a Metal Ion Transporter of Salmonella Typhimurium
… the control of these regulators. In addition, a RpoS mediated growth phase dependent regulation of the sit operon was observed. The sit locus is induced in the animal after invasion of the intestinal epithelium. We show that a sit null mutant is significantly attenuated in BALB/c and C3H/HeN …
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