Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"rolling circle replication"”.
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Autonomous replication of integrative and conjugative elements
… are transmitted to daughter cells by chromosomal replication and cell division. Under certain conditions, ICE DNA will excise and form a circular plasmid-like intermediate. It was previously thought that ICEs were incapable of autonomous replication. However, my research, along with the work of …
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Single stranded DNA: A new model system for single molecule polymer dynamics
… Using a biochemical synthesis scheme called rolling circle replication, long strands (>25 µm) of ssDNA containing “designer sequences” have been generated to prevent intramolecular base pairing. Polymer chains are synthesized with amine modified bases incorporated along the backbone to …
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Consequences of a mobile genetic element integrated at secondary locations
… due to DNA damage resulting from plasmid-like, rolling-circle replication of the excision-defective ICEBs1 in the chromosome. Consistent with this model, deletion of ICEBs] replication genes (nicK and helP) alleviated the proliferation and viability defects. Implications for the evolution of …
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Development of a potential challenge model and plant-produced vaccine candidate for beak and feather disease virus
… a BFDV dsDNA molecular clone was synthesised and replication assessed in 293TT mammalian cells and N. benthamiana using rolling circle replication and qPCR. Two distinct BFDV phylogenetic clusters were reported for Cape parrots, and a direct correlation was seen between viral load in the blood and …
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Expression Profiling by DNA Microarrays : Development of Amplification Methods for the Analysis of Minimal Tumor Samples
… a method was evaluated that uses on-chip rolling circle replication of circularized oligonucleotides for the amplified detection of gene expression profiles. This principle should allow for a faster and cheaper experimental procedure, circumventing sequence-dependent amplification bias. …
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Funktionelle Bedeutung der Protein-Kinase C eta im Infektionszyklus des autonomen Parvovirus Minute Virus of Mice
… Protein für die rollende Haarnadelreplikation (rolling circle replication) in vitro zu stimulieren. In der vorliegenden Arbeit konnte eine weitere zelluläre Kinase, PKCeta, identifiziert werden, die NS1 in vitro phosphoryliert und so das virale Polypeptid zusammen mit PKClambda für die rolling …
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Construction of plasmid-based expression and secretion vectors and study of the immobilization of proteins on the surface of Bacillus subtilis cells
… from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture supernatant. The author has also established an experimental …
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The design, development and characterization of a self-replicating DNA expression technology
… development and characterization of a self-replication DNA gene expression technology. This novel DNA expression system employs a form of DNA replication (known as rolling circle replication) to generate a self-replicating DNA amplicon that can amplify its own copy number and the relative …
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An Alternative View of the Templates and Functions of RNA Polymerase II
… in the RNA-templated RNA synthesis during replication of some Viroids and Hepatitis Delta Virus (HDV). In this study HDV RNA-templated pol II transcription was examined in vitro, using HeLa cell nuclear extracts (NE). Under standard conditions for the DNA-templated pol II transcription, a …