Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"ribosome stalling"”.
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The Impact of Depleting the Essential Bacterial Enzyme Peptidyl-tRNA Hydrolase on Translation
… free pep-tRNAs that dissociate from the ribosome during translation to prevent starvation for translation-usable tRNAs. Depletion of Pth leads to the accumulation of pep-tRNAs, rapid inhibition of translation, and cell death. Recent studies have demonstrated that Pth can process pep-tRNAs …
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The Roles of Codon Usage in Translational and Transcriptional Regulation on Gene Expression
… slow decoding rate of nonoptimal codon causes ribosome stalling on mRNA, which leads to premature termination of translation and reduced protein production. This process is conserved from Neurospora to Drosophila. In addition, I demonstrate that the premature termination of translation is …
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Mechanism of Translational Control by the Fragile X Mental Retardation Protein and Creation of the FMRP CTAG Mouse
… that is predominantly associated with polyribosomes. Loss of FMRP results in Fragile X Syndrome, characterized by mental retardation, autism and epilepsy. FMRP was recently found to be associated with a specific set of mRNAs with key roles in neuronal function and to physically interact …
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Magnesium regulates transcription of the mgtA (magnesium transporter) gene in Salmonella enterica serovar typhimurium via prolyl-bond formation during translation of the mgtL leader ORF
… prolyl-bond formation. We find that rescue of ribosome stalling at proline codons of <em>mgtL</em> by translation factor EF-P and methylation of tRNAPro with m1G37 by TrmD both play roles in the regulation of <em>mgtL</em>translation. Of potential significance is that TrmD is dependent on Mg2+ …
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Insights into the mechanisms of translation surveillance using constitutive RNA binding ASC-1 complex mutants
… stretches of problematic or damaged mRNA, ribosomes can slow, stall, and collide. Although ribosome stalling and collisions can be caused by numerous translation stresses, from mRNA damage to amino acid starvation, collided ribosomes exist abundantly even in the absence of overt translation …
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Essentiality and Regulation of Deoxyhypusination in Trypanosoma brucei
… Modified eIF5A has been shown to alleviate ribosome stalling on polyproline tracts. Human and yeast encode two isoforms of eIF5A but only one gene was identified in T. brucei (Tb927.11.740). Herein, I show that TbeIF5A and its modified lysine are essential for parasite growth by gene …
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Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
During mRNA translation, the ribosome protects ~28 nt of mRNA within its mRNA tunnel. Ribosome profiling is a method which takes advantage of this protected mRNA fragment, commonly referred to as the ribosome protected fragment (RPF). This method uses endonucleases to digest unprotected mRNA, …
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Translation-Mediated Stress Responses: Mining of Ribosome Profiling Data
… cellular mRNA fragments protected by translating ribosomes, dubbed ribosome profiling. This approach allows detection of differential translation activity, annotation of novel protein coding sequences and variants, identification of ribosome pause sites and estimates of de novo protein synthesis. …
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Molecular recognition of aberrant translation
… demanding process which requires several million ribosomes and consumes as much as 75% of cellular energy. Because of its importance, translation is regulated at many levels to maintain its high fidelity, with both substrates and synthesized products monitored by numerous quality control …
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RNA G-quadruplexes and polyamines effects on translation: data analysis and development of software for ribosome pause prediction
Ribosome profiling (Ribo-seq) technique provides a global snapshot of actively translating ribosomes by capturing the ribosome protected mRNA fragments. Ribo-seq technique can be implemented to study various translation aspects such as differential gene expression at the translation level, …